登録情報 データベース : PDB / ID : 6v9i 構造の表示 ダウンロードとリンクタイトル cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2) 要素Immunoglobulin G-binding protein G,Cullin-5 RING-box protein 2 詳細キーワード LIGASE / E3 Ubiquitin Ligase / RING-box protein機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
radial glia guided migration of Purkinje cell / cerebral cortex radially oriented cell migration / ERBB2 signaling pathway / Neddylation / Antigen processing: Ubiquitination & Proteasome degradation / cullin-RING ubiquitin ligase complex / protein neddylation / NEDD8 ligase activity / IgG binding / Cul5-RING ubiquitin ligase complex ... radial glia guided migration of Purkinje cell / cerebral cortex radially oriented cell migration / ERBB2 signaling pathway / Neddylation / Antigen processing: Ubiquitination & Proteasome degradation / cullin-RING ubiquitin ligase complex / protein neddylation / NEDD8 ligase activity / IgG binding / Cul5-RING ubiquitin ligase complex / SCF ubiquitin ligase complex / intrinsic apoptotic signaling pathway in response to oxidative stress / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / apoptotic mitochondrial changes / site of DNA damage / cullin family protein binding / intrinsic apoptotic signaling pathway / Vif-mediated degradation of APOBEC3G / calcium channel activity / Inactivation of CSF3 (G-CSF) signaling / Evasion by RSV of host interferon responses / Downregulation of ERBB2 signaling / G1/S transition of mitotic cell cycle / ubiquitin-protein transferase activity / activation of cysteine-type endopeptidase activity involved in apoptotic process / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation / protein-macromolecule adaptor activity / Neddylation / signaling receptor activity / ubiquitin-dependent protein catabolic process / protein ubiquitination / ubiquitin protein ligase binding / negative regulation of apoptotic process / zinc ion binding / extracellular region / nucleoplasm / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 IgG-binding B / B domain / M protein-type anchor domain / GA-like domain / GA-like domain / Zinc finger, RING-H2-type / RING-H2 zinc finger domain / Cullin protein neddylation domain / Cullin, conserved site / Cullin family signature. ... IgG-binding B / B domain / M protein-type anchor domain / GA-like domain / GA-like domain / Zinc finger, RING-H2-type / RING-H2 zinc finger domain / Cullin protein neddylation domain / Cullin, conserved site / Cullin family signature. / Cullin / Cullin repeat-like-containing domain superfamily / Cullin protein, neddylation domain / Cullin protein neddylation domain / Immunoglobulin/albumin-binding domain superfamily / Cullin / Cullin, N-terminal / Cullin homology domain / Cullin homology domain superfamily / Cullin family / Cullin family profile. / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. / LPXTG cell wall anchor domain / Zinc finger RING-type profile. / Zinc finger, RING-type / Zinc finger, RING/FYVE/PHD-type / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily 類似検索 - ドメイン・相同性 Immunoglobulin G-binding protein G / Cullin-5 / RING-box protein 2 類似検索 - 構成要素生物種 Streptococcus sp. group G (バクテリア)Homo sapiens (ヒト)Mus musculus (ハツカネズミ)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 5.2 Å 詳細データ登録者 Komives, E.A. / Lumpkin, R.J. / Baker, R.W. / Leschziner, A.E. 資金援助 米国, 1件 詳細 詳細を隠す組織 認可番号 国 National Science Foundation (NSF, United States) 1817774 米国
引用ジャーナル : Nat Commun / 年 : 2020タイトル : Structure and dynamics of the ASB9 CUL-RING E3 Ligase.著者 : Ryan J Lumpkin / Richard W Baker / Andres E Leschziner / Elizabeth A Komives / 要旨 : The Cullin 5 (CUL5) Ring E3 ligase uses adaptors Elongins B and C (ELOB/C) to bind different SOCS-box-containing substrate receptors, determining the substrate specificity of the ligase. The 18- ... The Cullin 5 (CUL5) Ring E3 ligase uses adaptors Elongins B and C (ELOB/C) to bind different SOCS-box-containing substrate receptors, determining the substrate specificity of the ligase. The 18-member ankyrin and SOCS box (ASB) family is the largest substrate receptor family. Here we report cryo-EM data for the substrate, creatine kinase (CKB) bound to ASB9-ELOB/C, and for full-length CUL5 bound to the RING protein, RBX2, which binds various E2s. To date, no full structures are available either for a substrate-bound ASB nor for CUL5. Hydrogen-deuterium exchange (HDX-MS) mapped onto a full structural model of the ligase revealed long-range allostery extending from the substrate through CUL5. We propose a revised allosteric mechanism for how CUL-E3 ligases function. ASB9 and CUL5 behave as rigid rods, connected through a hinge provided by ELOB/C transmitting long-range allosteric crosstalk from the substrate through CUL5 to the RBX2 flexible linker. 履歴 登録 2019年12月13日 登録サイト : RCSB / 処理サイト : RCSB改定 1.0 2020年4月29日 Provider : repository / タイプ : Initial release改定 1.1 2020年11月11日 Group : Database references / カテゴリ : citation / citation_authorItem : _citation.country / _citation.journal_abbrev ... _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name
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