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TitleStructure and dynamics of the ASB9 CUL-RING E3 Ligase.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 2866, Year 2020
Publish dateJun 8, 2020
AuthorsRyan J Lumpkin / Richard W Baker / Andres E Leschziner / Elizabeth A Komives /
PubMed AbstractThe Cullin 5 (CUL5) Ring E3 ligase uses adaptors Elongins B and C (ELOB/C) to bind different SOCS-box-containing substrate receptors, determining the substrate specificity of the ligase. The 18- ...The Cullin 5 (CUL5) Ring E3 ligase uses adaptors Elongins B and C (ELOB/C) to bind different SOCS-box-containing substrate receptors, determining the substrate specificity of the ligase. The 18-member ankyrin and SOCS box (ASB) family is the largest substrate receptor family. Here we report cryo-EM data for the substrate, creatine kinase (CKB) bound to ASB9-ELOB/C, and for full-length CUL5 bound to the RING protein, RBX2, which binds various E2s. To date, no full structures are available either for a substrate-bound ASB nor for CUL5. Hydrogen-deuterium exchange (HDX-MS) mapped onto a full structural model of the ligase revealed long-range allostery extending from the substrate through CUL5. We propose a revised allosteric mechanism for how CUL-E3 ligases function. ASB9 and CUL5 behave as rigid rods, connected through a hinge provided by ELOB/C transmitting long-range allosteric crosstalk from the substrate through CUL5 to the RBX2 flexible linker.
External linksNat Commun / PubMed:32513959 / PubMed Central
MethodsEM (single particle)
Resolution4.1 - 5.2 Å
Structure data

21120

6v9h

EMDB-21120, PDB-6v9h:
Ankyrin repeat and SOCS-box protein 9 (ASB9), ElonginB (ELOB), and ElonginC (ELOC) bound to its substrate Brain-type Creatine Kinase (CKB)
Method: EM (single particle) / Resolution: 4.1 Å

21121

6v9i

EMDB-21121, PDB-6v9i:
cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2)
Method: EM (single particle) / Resolution: 5.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • streptococcus sp. group g (bacteria)
KeywordsTRANSCRIPTION / Ankyrin repeat / Elongation Factor / Creatine Kinase / Ubiquitin / LIGASE / E3 Ubiquitin Ligase / RING-box protein

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