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- PDB-4my9: Crystal Structure of the Inosine 5'-monophosphate Dehydrogenase w... -

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Basic information

Entry
Database: PDB / ID: 4my9
TitleCrystal Structure of the Inosine 5'-monophosphate Dehydrogenase with an Internal Deletion of the CBS Domain from Bacillus anthracis str. Ames complexed with inhibitor C91
ComponentsInosine-5'-monophosphate dehydrogenase
KeywordsOXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR / Structural Genomics / NIAID / National Institute of Allergy and Infectious Diseases / Center for Structural Genomics of Infectious Diseases / CSGID / TIM barrel / alpha-beta structure / OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex
Function / homology
Function and homology information


IMP dehydrogenase / IMP dehydrogenase activity / GMP biosynthetic process / GTP biosynthetic process / nucleotide binding / metal ion binding
Similarity search - Function
IMP dehydrogenase / GMP reductase, conserved site / IMP dehydrogenase / GMP reductase signature. / Inosine-5'-monophosphate dehydrogenase / IMP dehydrogenase/GMP reductase / IMP dehydrogenase / GMP reductase domain / IMP dehydrogenase / GMP reductase domain / Domain in cystathionine beta-synthase and other proteins. / CBS domain superfamily / CBS domain / CBS domain ...IMP dehydrogenase / GMP reductase, conserved site / IMP dehydrogenase / GMP reductase signature. / Inosine-5'-monophosphate dehydrogenase / IMP dehydrogenase/GMP reductase / IMP dehydrogenase / GMP reductase domain / IMP dehydrogenase / GMP reductase domain / Domain in cystathionine beta-synthase and other proteins. / CBS domain superfamily / CBS domain / CBS domain / CBS domain profile. / Aldolase class I / Aldolase-type TIM barrel / TIM Barrel / Alpha-Beta Barrel / Alpha Beta
Similarity search - Domain/homology
Chem-C91 / INOSINIC ACID / MALONATE ION / Inosine-5'-monophosphate dehydrogenase / Inosine-5'-monophosphate dehydrogenase
Similarity search - Component
Biological speciesBacillus anthracis (anthrax bacterium)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5893 Å
AuthorsKim, Y. / Makowska-Grzyska, M. / Gu, M. / Gorla, S.K. / Hedstrom, L. / Anderson, W.F. / Joachimiak, A. / CSGID / Center for Structural Genomics of Infectious Diseases (CSGID)
CitationJournal: To be Published
Title: Crystal Structure of the Inosine 5'-monophosphate Dehydrogenase with an Internal Deletion of the CBS Domain from Bacillus anthracis str. Ames complexed with inhibitor C91
Authors: Kim, Y. / Makowska-Grzyska, M. / Gu, M. / Anderson, W.F. / Joachimiak, A.
History
DepositionSep 27, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 11, 2014Provider: repository / Type: Initial release
Revision 1.1May 17, 2017Group: Database references / Structure summary
Revision 1.2Jan 24, 2018Group: Structure summary / Category: audit_author / Item: _audit_author.name
Revision 1.3Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Inosine-5'-monophosphate dehydrogenase
B: Inosine-5'-monophosphate dehydrogenase
C: Inosine-5'-monophosphate dehydrogenase
D: Inosine-5'-monophosphate dehydrogenase
E: Inosine-5'-monophosphate dehydrogenase
F: Inosine-5'-monophosphate dehydrogenase
G: Inosine-5'-monophosphate dehydrogenase
H: Inosine-5'-monophosphate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)332,40833
Polymers325,6778
Non-polymers6,73125
Water5,585310
1
A: Inosine-5'-monophosphate dehydrogenase
B: Inosine-5'-monophosphate dehydrogenase
C: Inosine-5'-monophosphate dehydrogenase
D: Inosine-5'-monophosphate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)166,35718
Polymers162,8384
Non-polymers3,51914
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area25190 Å2
ΔGint-114 kcal/mol
Surface area46340 Å2
MethodPISA
2
E: Inosine-5'-monophosphate dehydrogenase
F: Inosine-5'-monophosphate dehydrogenase
G: Inosine-5'-monophosphate dehydrogenase
H: Inosine-5'-monophosphate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)166,05115
Polymers162,8384
Non-polymers3,21311
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24470 Å2
ΔGint-119 kcal/mol
Surface area46090 Å2
MethodPISA
Unit cell
Length a, b, c (Å)84.930, 89.883, 104.621
Angle α, β, γ (deg.)98.70, 90.32, 96.46
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
Inosine-5'-monophosphate dehydrogenase / IMP dehydrogenase / IMPD / IMPDH


Mass: 40709.574 Da / Num. of mol.: 8 / Fragment: IMPDH-delt-L-CBS / Mutation: replaced residues 92-220 with GG
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bacillus anthracis (anthrax bacterium) / Strain: Ames / Gene: guaB, BA_0008, BAS0011, GBAA_0008 / Plasmid: pMCSG7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 magic
References: UniProt: Q81W29, UniProt: A0A6L8P2U9*PLUS, IMP dehydrogenase
#2: Chemical
ChemComp-IMP / INOSINIC ACID


Mass: 348.206 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C10H13N4O8P
#3: Chemical
ChemComp-C91 / N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide


Mass: 378.426 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C24H18N4O
#4: Chemical
ChemComp-MLI / MALONATE ION


Mass: 102.046 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C3H2O4
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 310 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.41 Å3/Da / Density % sol: 48.92 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 5 % w/v Tacsimate, 0.1 M HEPES, pH 7.0, 10% w/v PEGMME 5000, VAPOR DIFFUSION, SITTING DROP, temperature 289K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97921 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 16, 2012 / Details: mirrors
RadiationMonochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97921 Å / Relative weight: 1
ReflectionResolution: 2.589→50 Å / Num. all: 91462 / Num. obs: 91462 / % possible obs: 98.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 1.9 % / Biso Wilson estimate: 46.8 Å2 / Rsym value: 0.081 / Net I/σ(I): 11.1
Reflection shellResolution: 2.589→2.64 Å / Redundancy: 1.9 % / Mean I/σ(I) obs: 2.2 / Num. unique all: 4515 / Rsym value: 0.407 / % possible all: 97.1

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Processing

Software
NameVersionClassification
SBC-Collectdata collection
HKL-3000data collection
HKL-3000phasing
MOLREPphasing
PHENIX(phenix.refine: 1.8.1_1161)refinement
HKL-3000data reduction
HKL-3000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDBID ENTRY 3STD

3std


Resolution: 2.5893→38.748 Å / SU ML: 0.32 / Isotropic thermal model: mixed / Cross valid method: THROUGHOUT / Phase error: 25.69 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.216 4583 5.02 %random
Rwork0.17 ---
all0.172 91344 --
obs0.172 91344 96.6 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.3 Å2
Refinement stepCycle: LAST / Resolution: 2.5893→38.748 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20464 0 479 310 21253
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00321280
X-RAY DIFFRACTIONf_angle_d0.7428803
X-RAY DIFFRACTIONf_dihedral_angle_d13.9827796
X-RAY DIFFRACTIONf_chiral_restr0.0463327
X-RAY DIFFRACTIONf_plane_restr0.0033700
LS refinement shell

Refine-ID: X-RAY DIFFRACTION

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection obs% reflection obs (%)
2.5893-2.61880.3478780.31241613169154
2.6188-2.64960.33851680.26762962313097
2.6496-2.68190.30721470.25712855300298
2.6819-2.71580.3011770.25182929310698
2.7158-2.75150.2981720.24672890306298
2.7515-2.78920.3321560.24872905306198
2.7892-2.8290.31161560.23732996315297
2.829-2.87130.29111340.23112884301898
2.8713-2.91610.31221790.22342894307398
2.9161-2.96390.29321440.22642924306498
2.9639-3.0150.2821470.22292955310298
3.015-3.06980.27671450.22332977312298
3.0698-3.12880.2941540.21142897305198
3.1288-3.19260.26621650.20392931309698
3.1926-3.2620.23111490.19312958310798
3.262-3.33790.27281530.18622912306598
3.3379-3.42130.23041690.19192908307798
3.4213-3.51370.20781530.17292965311898
3.5137-3.61710.25431610.17912927308898
3.6171-3.73370.21721530.16242950310398
3.7337-3.86710.1991510.16212972312399
3.8671-4.02170.20731380.15192920305898
4.0217-4.20460.20291650.1392957312299
4.2046-4.42590.15951350.1336293830798
4.4259-4.70280.15771690.12822986315599
4.7028-5.06520.16131440.11982948309299
5.0652-5.57360.17431720.13862934310699
5.5736-6.3770.19091580.15012977313599
6.377-8.02260.15021350.14232971310699
8.0226-38.75210.15461560.14062926308298
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.5651-0.15380.16521.09370.12120.8138-0.05170.04050.0248-0.15940.03820.1821-0.0385-0.15970.02130.3459-0.0225-0.03350.32460.03580.22681.94465.4019-2.0165
20.9503-0.15020.18691.0122-0.27621.21640.02340.045-0.2071-0.2065-0.0640.1720.1078-0.21190.01360.3593-0.0486-0.03960.3407-0.03210.29780.7559-4.4139-4.5226
34.8518-1.59040.10191.28520.07831.3796-0.4799-0.5997-0.30830.4990.35460.2370.0003-0.09010.19860.3580.00480.07310.38750.06610.2928-0.1524-3.318417.1511
40.74340.3662-0.02531.3663-0.06831.30390.03650.0143-0.1079-0.184-0.02980.1239-0.1707-0.1380.01180.36680.0485-0.03280.2706-0.01010.2525.19718.56113.8158
51.74271.1861.03981.84710.83921.2071-0.17610.0768-0.0817-0.39680.0917-0.3062-0.27480.21830.12520.4113-0.03320.09010.28750.0580.293325.901524.8597-1.082
62.58540.483-0.50041.3748-0.1121.25530.0035-0.04750.5353-0.19830.00790.1775-0.2530.1740.02350.48880.03710.08350.3030.01360.390819.231346.265810.9014
71.7356-0.4694-0.05071.7729-0.27641.80430.2624-0.00070.00660.07750.01530.4237-0.6463-0.4176-0.25970.67580.16150.04860.32360.03820.47293.394748.996812.3261
80.5581-0.43240.07181.51020.4180.2117-0.04580.14150.1541-0.4040.14410.3777-0.3122-0.19-0.09890.69870.0802-0.0370.33760.07980.35366.183142.2679-3.3948
90.72220.2065-0.14780.8397-0.14990.5379-0.0440.07950.092-0.11740.1022-0.0173-0.3992-0.1427-0.07470.45230.00970.02210.2630.03210.283517.692133.39383.4854
101.73810.5491-0.30751.90590.25141.57880.1887-0.0265-0.03160.2765-0.12690.1376-0.1917-0.17060.00820.41450.02380.05990.28430.0180.27186.962932.950923.6048
111.59150.7877-0.37081.44781.19841.62890.1566-0.25880.26920.0198-0.14540.1951-0.15740.02970.01980.42720.0393-0.02280.18870.06670.306917.456435.335817.8504
121.3710.34390.51231.38850.39281.1167-0.07370.11790.0154-0.01260.0948-0.0297-0.1782-0.0172-0.04090.3052-0.02880.05020.25490.04630.287631.308629.456613.8752
131.69160.7587-0.05190.6707-0.12050.3986-0.03450.0867-0.198-0.1650.0222-0.291-0.07640.10680.00470.31860.02320.03240.24340.0150.220332.20920.5808-0.2493
140.93230.01320.24021.32580.04981.51720.0598-0.068-0.19880.32250.0682-0.28460.39520.2011-0.13720.53150.0926-0.02720.2850.00930.406938.381-24.683416.9596
150.18720.6988-0.05962.6023-0.21740.1102-0.112-0.0474-0.04550.22660.2003-0.54480.03510.1639-0.09250.39750.10210.02950.3935-0.03630.602751.2159-17.054411.4406
160.32940.43340.25740.903-0.03120.6638-0.0613-0.0703-0.1506-0.08990.0642-0.1380.0410.1795-0.01850.36230.04620.05090.3064-0.04280.345440.4571-10.30026.6834
171.07180.54490.33661.6944-0.09431.1330.1591-0.2133-0.06250.384-0.08030.00730.2802-0.0046-0.01770.38050.00710.02210.23040.0120.274428.6125-12.561721.6897
181.3428-0.10710.3250.8838-0.31331.5831-0.0571-0.00780.03070.22030.0924-0.0882-0.0468-0.152-0.00780.3538-0.0215-0.01410.257-0.01340.249219.1976-5.45766.1399
190.8387-0.06950.00270.69520.14170.7891-0.05370.01570.03960.09830.0441-0.2707-0.13870.2102-0.02460.2694-0.0299-0.02240.3230.01250.384350.202718.830718.4363
201.1918-0.20380.27881.27250.30670.0996-0.01430.02030.12520.12840.0542-0.399-0.22760.247-0.01640.3649-0.1115-0.02070.3656-0.00780.431852.306929.954218.4516
211.8439-0.95590.33811.2163-0.27930.9654-0.3181-0.3343-0.15530.45340.17240.0135-0.11080.01540.1750.4746-0.0746-0.02260.3415-0.02320.371442.193218.863332.8535
221.242-0.09340.54940.9119-0.39971.6868-0.0665-0.30410.07150.35150.0888-0.166-0.26110.14320.05110.3775-0.0672-0.04360.297-0.00280.380743.630218.034127.441
231.3286-0.3490.23431.86790.48441.42130.08450.10650.1520.005-0.0581-0.2569-0.11540.0193-0.04020.27580.01520.0180.2296-0.00670.287741.69285.023815.863
240.84380.00310.0480.25320.1780.8298-0.06230.19740.0872-0.15680.0189-0.1030.1608-0.03260.03060.42440.05570.05370.35710.07670.23974.910113.462549.6864
251.1946-0.3690.06662.66120.81991.6641-0.041-0.16990.24260.40710.0237-0.50490.14380.58110.0580.30510.01250.00140.42720.00710.44924.107123.798561.0387
261.1883-0.1413-0.34550.20390.00070.83940.07010.14060.3001-0.0906-0.1245-0.1977-0.10110.1590.050.43250.04690.04570.40550.10530.37528.647124.971452.0512
273.0296-0.2089-0.25611.42470.08652.3318-0.2266-0.40850.05130.80060.0757-0.22450.40460.23130.10060.51220.0432-0.0190.45570.04220.365311.60117.329973.3783
281.3129-0.15710.38481.14640.39380.87010.05740.02740.0306-0.0988-0.0132-0.13010.06390.0026-0.02790.41110.0880.06850.34840.01180.26865.65240.880654.1248
291.44030.6246-0.04771.31-0.03170.5346-0.15180.20360.1726-0.28340.06920.18030.0581-0.0790.04220.42950.05590.00370.35010.08620.307-21.827319.209355.2671
301.4880.03070.02811.3982-0.1171.1679-0.0561-0.19310.25360.26050.00250.2172-0.224-0.1870.0070.51390.11160.07940.3576-0.02050.4215-25.021238.578979.0744
310.42640.4538-0.1811.22720.36080.34320.00360.03280.14020.0363-0.03090.2911-0.1846-0.25440.01350.39720.15870.06060.37710.06410.3987-31.900130.413968.1307
321.39540.07030.68152.28861.10521.34050.0958-0.15920.0440.55250.1543-0.24260.1646-0.2987-0.13650.45280.07050.00070.43390.00680.3588-18.849322.675587.1401
331.00040.018-0.01530.9440.2411.80010.0763-0.1919-0.03840.1054-0.21850.0772-0.25710.1410.0540.32910.06890.00830.1861-0.0160.2964-14.881527.422476.2438
341.40560.381-0.01030.92370.20811.1122-0.06660.03490.01340.15840.00740.15510.06050.14020.08880.34010.07660.04530.29840.06710.2688-7.962723.385163.4032
351.82711.06730.08291.77-0.27480.3728-0.05460.0471-0.025-0.22-0.0434-0.07560.0092-0.03870.04390.43550.1186-0.03940.3183-0.03440.2515-14.5763-3.0447.1342
361.4678-0.3125-0.13771.0238-0.30591.5778-0.174-0.0915-0.3402-0.28380.0725-0.24260.43140.21980.05310.52960.07850.02710.2907-0.03870.3811-0.3072-29.779354.2756
370.0877-0.13540.21761.4789-0.43830.68210.09150.1831-0.0982-0.2585-0.1131-0.21780.42430.2952-0.01860.64850.15530.12610.5496-0.04950.43289.4918-24.50945.3367
380.3388-0.11790.19461.85160.14010.41610.03650.1613-0.1208-0.3549-0.0269-0.02340.21890.06510.00250.55970.04990.02610.4229-0.03270.2851-3.1155-15.414946.1997
390.6220.366-0.32313.3037-1.37592.4717-0.0119-0.244-0.18270.2382-0.2286-0.44730.01620.27460.27590.37840.0892-0.00590.3537-0.01610.33055.075-17.056167.9572
401.5272-0.0035-0.19611.587-0.23270.3656-0.09290.0461-0.0572-0.01840.10640.16850.22630.039-0.05570.375-0.0043-0.00950.31010.00690.2667-19.7911-15.818360.4686
411.0348-0.2133-0.17190.85940.20770.41540.12680.1740.051-0.182-0.1810.1317-0.2195-0.35730.06410.37390.1148-0.00630.48270.06990.4911-36.09773.678659.581
421.1578-0.19110.27751.13790.34811.02220.022-0.1511-0.15320.25650.04520.30850.2821-0.3059-0.02370.457-0.09040.08560.48660.0330.4864-45.553-16.655176.5404
430.6265-0.1147-0.66640.2014-0.07880.9174-0.01510.1442-0.23540.0536-0.09930.1540.20690.0240.14280.4832-0.1027-0.02690.5761-0.07760.6323-44.3269-20.54961.3362
440.51-0.08060.14980.9093-0.0570.8612-0.02590.15640.1314-0.0599-0.07880.28580.2692-0.27190.11830.3098-0.0364-0.00160.45420.00470.3468-37.5328-7.507560.7807
452.1955-0.6314-1.68831.93450.43271.5104-0.2112-0.5827-0.12990.38160.08970.11890.15730.3018-0.01790.5223-0.03830.03530.47050.01380.3539-31.3257-7.572381.7065
461.1442-0.2484-0.8521.08841.03012.5941-0.0788-0.1073-0.20890.37180.0306-0.0010.45990.176-0.01930.56720.0240.01660.38690.0220.3878-26.9046-15.98577.2408
470.46590.2017-0.27791.5039-0.10521.1379-0.06880.011-0.05920.1528-0.06220.1941-0.0052-0.12460.12270.28980.02580.06170.35570.03120.327-33.55829.805771.6211
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid -2 through 77 )
2X-RAY DIFFRACTION2chain 'A' and (resid 78 through 368 )
3X-RAY DIFFRACTION3chain 'A' and (resid 369 through 427 )
4X-RAY DIFFRACTION4chain 'A' and (resid 428 through 486 )
5X-RAY DIFFRACTION5chain 'B' and (resid -1 through 26 )
6X-RAY DIFFRACTION6chain 'B' and (resid 27 through 56 )
7X-RAY DIFFRACTION7chain 'B' and (resid 57 through 242 )
8X-RAY DIFFRACTION8chain 'B' and (resid 243 through 293 )
9X-RAY DIFFRACTION9chain 'B' and (resid 294 through 363 )
10X-RAY DIFFRACTION10chain 'B' and (resid 364 through 400 )
11X-RAY DIFFRACTION11chain 'B' and (resid 401 through 446 )
12X-RAY DIFFRACTION12chain 'B' and (resid 447 through 486 )
13X-RAY DIFFRACTION13chain 'C' and (resid 0 through 26 )
14X-RAY DIFFRACTION14chain 'C' and (resid 27 through 242 )
15X-RAY DIFFRACTION15chain 'C' and (resid 243 through 268 )
16X-RAY DIFFRACTION16chain 'C' and (resid 269 through 355 )
17X-RAY DIFFRACTION17chain 'C' and (resid 356 through 446 )
18X-RAY DIFFRACTION18chain 'C' and (resid 447 through 486 )
19X-RAY DIFFRACTION19chain 'D' and (resid -1 through 77 )
20X-RAY DIFFRACTION20chain 'D' and (resid 78 through 355 )
21X-RAY DIFFRACTION21chain 'D' and (resid 356 through 389 )
22X-RAY DIFFRACTION22chain 'D' and (resid 390 through 446 )
23X-RAY DIFFRACTION23chain 'D' and (resid 447 through 486 )
24X-RAY DIFFRACTION24chain 'E' and (resid -2 through 56 )
25X-RAY DIFFRACTION25chain 'E' and (resid 57 through 90 )
26X-RAY DIFFRACTION26chain 'E' and (resid 91 through 368 )
27X-RAY DIFFRACTION27chain 'E' and (resid 369 through 427 )
28X-RAY DIFFRACTION28chain 'E' and (resid 428 through 486 )
29X-RAY DIFFRACTION29chain 'F' and (resid -1 through 26 )
30X-RAY DIFFRACTION30chain 'F' and (resid 27 through 232 )
31X-RAY DIFFRACTION31chain 'F' and (resid 233 through 363 )
32X-RAY DIFFRACTION32chain 'F' and (resid 364 through 398 )
33X-RAY DIFFRACTION33chain 'F' and (resid 399 through 446 )
34X-RAY DIFFRACTION34chain 'F' and (resid 447 through 486 )
35X-RAY DIFFRACTION35chain 'G' and (resid -2 through 26 )
36X-RAY DIFFRACTION36chain 'G' and (resid 27 through 230 )
37X-RAY DIFFRACTION37chain 'G' and (resid 231 through 257 )
38X-RAY DIFFRACTION38chain 'G' and (resid 258 through 368 )
39X-RAY DIFFRACTION39chain 'G' and (resid 369 through 427 )
40X-RAY DIFFRACTION40chain 'G' and (resid 428 through 486 )
41X-RAY DIFFRACTION41chain 'H' and (resid -1 through 39 )
42X-RAY DIFFRACTION42chain 'H' and (resid 40 through 242 )
43X-RAY DIFFRACTION43chain 'H' and (resid 243 through 270 )
44X-RAY DIFFRACTION44chain 'H' and (resid 271 through 355 )
45X-RAY DIFFRACTION45chain 'H' and (resid 356 through 389 )
46X-RAY DIFFRACTION46chain 'H' and (resid 390 through 427 )
47X-RAY DIFFRACTION47chain 'H' and (resid 428 through 486 )

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