- PDB-1vf5: Crystal Structure of Cytochrome b6f Complex from M.laminosus -
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基本情報
登録情報
データベース: PDB / ID: 1vf5
タイトル
Crystal Structure of Cytochrome b6f Complex from M.laminosus
要素
CYTOCHROME B6
CYTOCHROME F
PROTEIN PET G
PROTEIN PET L
PROTEIN PET M
PROTEIN PET N
RIESKE IRON-SULFUR PROTEIN
SUBUNIT IV
キーワード
PHOTOSYNTHESIS / membrane protein complex / electron transfer complex
機能・相同性
機能・相同性情報
plastoquinol--plastocyanin reductase activity / cytochrome b6f complex / plastoquinol-plastocyanin reductase / electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity / cytochrome complex assembly / photosynthetic electron transport chain / ubiquinol-cytochrome-c reductase activity / plasma membrane-derived thylakoid membrane / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthesis ...plastoquinol--plastocyanin reductase activity / cytochrome b6f complex / plastoquinol-plastocyanin reductase / electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity / cytochrome complex assembly / photosynthetic electron transport chain / ubiquinol-cytochrome-c reductase activity / plasma membrane-derived thylakoid membrane / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthesis / respiratory electron transport chain / 2 iron, 2 sulfur cluster binding / electron transfer activity / iron ion binding / heme binding / metal ion binding 類似検索 - 分子機能
Single helix bin / plastocyanin oxidoreductase / Cytochrome b6-f complex subunit 6 / Cytochrome b6-f complex iron-sulfur subunit / Cytochrome b6/f complex, subunit 5 / Cytochrome b6-f complex, subunit 8 / Cytochrome b6/f complex, subunit IV / PetM of cytochrome b6/f complex subunit 7 / Cytochrome b6, PetB / Cytochrome b6/f complex, subunit 5 superfamily ...Single helix bin / plastocyanin oxidoreductase / Cytochrome b6-f complex subunit 6 / Cytochrome b6-f complex iron-sulfur subunit / Cytochrome b6/f complex, subunit 5 / Cytochrome b6-f complex, subunit 8 / Cytochrome b6/f complex, subunit IV / PetM of cytochrome b6/f complex subunit 7 / Cytochrome b6, PetB / Cytochrome b6/f complex, subunit 5 superfamily / Cytochrome b6-f complex, subunit 8 superfamily / Cytochrome B6-F complex subunit 5 / PetN / PetM family of cytochrome b6f complex subunit 7 / Cytochrome f large domain / Cytochrome f transmembrane anchor / Cytochrome f / Cytochrome f large domain / Cytochrome f large domain superfamily / Apocytochrome F, C-terminal / Apocytochrome F, N-terminal / Cytochrome f family profile. / Single helix bin / Cytochrome Bc1 Complex; Chain C / Cytochrome Bc1 Complex; Chain C / Rieske Iron-sulfur Protein / Rieske [2Fe-2S] iron-sulphur domain / 3-layer Sandwich / : / : / Cytochrome b/b6, C-terminal / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6 C-terminal region profile. / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Rieske iron-sulphur protein, C-terminal / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / Di-haem cytochrome, transmembrane / Rudiment single hybrid motif / Rieske iron-sulphur protein / Rieske [2Fe-2S] iron-sulphur domain / Rieske [2Fe-2S] domain / Rieske [2Fe-2S] iron-sulfur domain profile. / Rieske [2Fe-2S] iron-sulphur domain superfamily / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Helix Hairpins / Up-down Bundle / Immunoglobulin-like / Beta Barrel / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha 類似検索 - ドメイン・相同性
#1: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 2003 タイトル: A Defined Protein-Detergent-Lipid Complex for Crystallization of Integral Membrane Proteins: The Cytochrome B6F Complex of Oxygenic Photosynthesis 著者: Zhang, H. / Kurisu, G. / Smith, J.L. / Cramer, W.A.
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (1)
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集合体
登録構造単位
A: CYTOCHROME B6 B: SUBUNIT IV C: CYTOCHROME F D: RIESKE IRON-SULFUR PROTEIN E: PROTEIN PET L F: PROTEIN PET M G: PROTEIN PET G H: PROTEIN PET N N: CYTOCHROME B6 O: SUBUNIT IV P: CYTOCHROME F Q: RIESKE IRON-SULFUR PROTEIN R: PROTEIN PET L S: PROTEIN PET M T: PROTEIN PET G U: PROTEIN PET N ヘテロ分子
モノクロメーター: SI(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.0332 Å / 相対比: 1
反射
解像度: 3→150 Å / Num. obs: 100622 / % possible obs: 99.8 % / Observed criterion σ(I): -2 / 冗長度: 7 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 17.9
反射 シェル
解像度: 3→3.11 Å / Rmerge(I) obs: 0.54 / Mean I/σ(I) obs: 2.2 / % possible all: 100
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解析
ソフトウェア
名称
バージョン
分類
HKL-2000
データ収集
HKL-2000
データ削減
MLPHARE
位相決定
CNS
1.1
精密化
HKL-2000
データスケーリング
精密化
構造決定の手法: 多重同系置換 / 解像度: 3→48.16 Å / Data cutoff high absF: 2928724.46 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: ENGH & HUBER 詳細: Twinning was treated during refinement and the twinning fraction set to 0.50.