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- PDB-1egv: CRYSTAL STRUCTURE OF THE DIOL DEHYDRATASE-ADENINYLPENTYLCOBALAMIN... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1egv | ||||||
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Title | CRYSTAL STRUCTURE OF THE DIOL DEHYDRATASE-ADENINYLPENTYLCOBALAMIN COMPLEX FROM KLEBSELLA OXYTOCA UNDER THE ILLUMINATED CONDITION. | ||||||
![]() | (PROPANEDIOL DEHYDRATASE) x 3 | ||||||
![]() | LYASE / COENZYME B12 / PROPANEDIOL / POTASSIUM ION / TIM BARREL | ||||||
Function / homology | ![]() propanediol dehydratase / propanediol dehydratase activity / cobalamin binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Masuda, J. / Shibata, N. / Toraya, T. / Morimoto, Y. / Yasuoka, N. | ||||||
![]() | ![]() Title: How a protein generates a catalytic radical from coenzyme B(12): X-ray structure of a diol-dehydratase-adeninylpentylcobalamin complex. Authors: Masuda, J. / Shibata, N. / Morimoto, Y. / Toraya, T. / Yasuoka, N. #1: ![]() Title: A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase Authors: Shibata, N. / Masuda, J. / Toraya, T. / Morimoto, Y. / Yasuoka, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 401.1 KB | Display | ![]() |
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PDB format | ![]() | 316 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The protein exists as a dimer of heterotrimers. |
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Components
-Protein , 3 types, 6 molecules ALBEGM
#1: Protein | Mass: 60408.133 Da / Num. of mol.: 2 / Fragment: ALPHA CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 24141.678 Da / Num. of mol.: 2 / Fragment: BETA CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein | Mass: 19198.695 Da / Num. of mol.: 2 / Fragment: GAMMA CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 4 types, 1857 molecules ![](data/chem/img/K.gif)
![](data/chem/img/COY.gif)
![](data/chem/img/PGO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/COY.gif)
![](data/chem/img/PGO.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-K / #5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.77 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion sandwich drop / pH: 8 Details: PEG 6000, potassium phospate, Tris-HCl, LDAO, ammonium sulfate, propane diol, adeninlpentylcobalamin,, pH 8.0, VAPOR DIFFUSION sandwich drop, temperature 277K | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sandwich drop / Details: Masuda, J., (1999) Acta Crystallogr, D55, 907. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 12, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.7 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→100 Å / Num. all: 188943 / Num. obs: 151116 / % possible obs: 80 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4.65 % / Biso Wilson estimate: 15.4 Å2 / Rmerge(I) obs: 0.063 / Net I/σ(I): 18.37 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 3.13 % / Rmerge(I) obs: 0.119 / Num. unique all: 10109 / % possible all: 54.1 |
Reflection | *PLUS Num. measured all: 2393925 |
Reflection shell | *PLUS % possible obs: 54.1 % |
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Processing
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Refinement | Resolution: 1.75→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.75→30 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 30 Å / σ(F): 0 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |