- EMDB-10958: The structure of the small subunit of the mitoribosome from Neuro... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-10958
タイトル
The structure of the small subunit of the mitoribosome from Neurospora crassa
マップデータ
Composite map made out of following maps: head, body and tail.
試料
複合体: Mitoribosome of Neurospora crassa
タンパク質・ペプチド: x 35種
RNA: x 1種
リガンド: x 3種
機能・相同性
機能・相同性情報
3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / nuclear pore inner ring / nuclear pore organization / ATPase inhibitor activity / negative regulation of ATP-dependent activity / positive regulation of translational fidelity / mitochondrial large ribosomal subunit / structural constituent of nuclear pore / mitochondrial small ribosomal subunit ...3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / nuclear pore inner ring / nuclear pore organization / ATPase inhibitor activity / negative regulation of ATP-dependent activity / positive regulation of translational fidelity / mitochondrial large ribosomal subunit / structural constituent of nuclear pore / mitochondrial small ribosomal subunit / mitochondrial translation / superoxide dismutase activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / mitochondrion / RNA binding / metal ion binding / cytoplasm 類似検索 - 分子機能
: / Ribosomal protein L31p, N-terminal / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial ...: / Ribosomal protein L31p, N-terminal / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / IGR protein motif / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / ClpP/crotonase-like domain superfamily / Sterile alpha motif/pointed domain superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein S13, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal / Ribosomal protein S13, conserved site / Ribosomal protein S13 signature. / Ribosomal protein S5, C-terminal domain / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S13/S18 / Ribosomal protein S13 family profile. / Ribosomal protein S4, conserved site / Ribosomal protein S4 signature. / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S4/S9 / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / Ribosomal protein S10p/S20e / Ribosomal protein S11 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S10p/S20e / Ribosomal protein S9, conserved site / Ribosomal protein S9 signature. / Ribosomal protein S13-like, H2TH / S4 RNA-binding domain / S4 domain / Ribosomal protein S11 類似検索 - ドメイン・相同性
37S ribosomal protein mrp5 / Ribosomal protein S16, mitochondrial / Ribosomal protein S5, mitochondrial / 37S ribosomal protein S5 / Ribosomal protein S15 / Mitochondrial 37S ribosomal protein S27 / 37S ribosomal protein S24, mitochondrial / Protein FYV4, mitochondrial / Mitochondrial 3-hydroxyisobutyryl-CoA hydrolase / Large ribosomal subunit protein bL31m ...37S ribosomal protein mrp5 / Ribosomal protein S16, mitochondrial / Ribosomal protein S5, mitochondrial / 37S ribosomal protein S5 / Ribosomal protein S15 / Mitochondrial 37S ribosomal protein S27 / 37S ribosomal protein S24, mitochondrial / Protein FYV4, mitochondrial / Mitochondrial 3-hydroxyisobutyryl-CoA hydrolase / Large ribosomal subunit protein bL31m / Mito ribosomal protein S18 / Mitochondrial ribosomal protein S19 / 37S ribosomal protein S10, mitochondrial / Small ribosomal subunit protein mS27 / 40S ribosomal protein S13 / Fe superoxide dismutase / Mitochondrial 37S ribosomal protein S17 / 37S ribosomal protein mrp10, mitochondrial / Ribosomal_S7 domain-containing protein / 37S ribosomal protein S9, mitochondrial / Mitochondrial 30S ribosomal protein S12 / Mito ribosomal protein S4 / Mito ribosomal protein S21 / Mito ribosomal protein S6 / Small ribosomal subunit protein mS29 / Mitochondrial 40S ribosomal protein MRP2 / Protein FYV4, mitochondrial / Mitochondrial ribosomal protein subunit S18 / Small ribosomal subunit protein mS45 / 40S ribosomal protein S8 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS2m / ATP synthase F1 subunit epsilon 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.