CENP-A nucleosome: DNA / Histone H3-like centromeric protein A / Histone H4 / (Histone H2A type 2- ...) x 2 / Histone H2B type 1-C/E/F/G/I / (nucleic-acidNucleic acid) x 2
Function / homology
Function and homology information
condensed chromosome inner kinetochore / kinetochore assembly / protein localization to chromosome, centromeric region / pericentric heterochromatin => GO:0005721 / condensed nuclear chromosome kinetochore / condensed chromosome, centromeric region => GO:0000779 / establishment of mitotic spindle orientation / negative regulation of megakaryocyte differentiation / chromosome, centromeric region / CENP-A containing nucleosome assembly ...condensed chromosome inner kinetochore / kinetochore assembly / protein localization to chromosome, centromeric region / pericentric heterochromatin => GO:0005721 / condensed nuclear chromosome kinetochore / condensed chromosome, centromeric region => GO:0000779 / establishment of mitotic spindle orientation / negative regulation of megakaryocyte differentiation / chromosome, centromeric region / CENP-A containing nucleosome assembly / DNA replication-independent nucleosome assembly / telomere capping / mitotic cytokinesis / chromatin silencing / telomere organization / DNA replication-dependent nucleosome assembly / nucleosome => GO:0000786 / innate immune response in mucosa / rDNA heterochromatin assembly / negative regulation of gene expression, epigenetic / regulation of gene silencing by miRNA / nuclear chromosome / DNA-templated transcription, initiation / regulation of megakaryocyte differentiation / nucleosome assembly / nucleosome / double-strand break repair via nonhomologous end joining / chromatin organization / chromosome, telomeric region => GO:0000781 / antibacterial humoral response / antimicrobial humoral immune response mediated by antimicrobial peptide / protein ubiquitination / protein heterodimerization activity / defense response to Gram-positive bacterium / chromatin => GO:0000785 / protein domain specific binding / chromatin binding / cellular protein metabolic process / viral process / protein-containing complex / RNA binding / DNA binding / extracellular space / extracellular exosome / membrane / extracellular region / nucleoplasm / identical protein binding / nucleus / cytosol
Journal: EMBO Rep / Year: 2019 Title: CENP-C unwraps the human CENP-A nucleosome through the H2A C-terminal tail. Authors: Ahmad Ali-Ahmad / Silvija Bilokapić / Ingmar B Schäfer / Mario Halić / Nikolina Sekulić / Abstract: Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is ...Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is considered to be a central organizer of the CCAN. We provide new molecular insights into the structure of human CENP-A nucleosomes, in isolation and in complex with the CENP-C central region (CENP-C ), the main CENP-A binding module of human CENP-C. We establish that the short αN helix of CENP-A promotes DNA flexibility at the nucleosome ends, independently of the sequence it wraps. Furthermore, we show that, in vitro, two regions of human CENP-C (CENP-C and CENP-C ) both bind exclusively to the CENP-A nucleosome. We find CENP-C to bind with high affinity due to an extended hydrophobic area made up of CENP-A and CENP-A . Importantly, we identify two key conformational changes within the CENP-A nucleosome upon CENP-C binding. First, the loose DNA wrapping of CENP-A nucleosomes is further exacerbated, through destabilization of the H2A C-terminal tail. Second, CENP-C rigidifies the N-terminal tail of H4 in the conformation favoring H4 monomethylation, essential for a functional centromere.
Name: CENP-A nucleosome / Number of components: 10
+
Component #1: protein, CENP-A nucleosome
Protein
Name: CENP-A nucleosome / Recombinant expression: No
+
Component #2: protein, CENP-A nucleosome
Protein
Name: CENP-A nucleosome / Recombinant expression: No
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #3: protein, DNA
Protein
Name: DNA / Recombinant expression: No
Source
Species: synthetic construct (others)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #4: protein, Histone H3-like centromeric protein A
Protein
Name: Histone H3-like centromeric protein A / Number of Copies: 2 / Recombinant expression: No
Mass
Theoretical: 16.02363 kDa
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #5: protein, Histone H4
Protein
Name: Histone H4 / Number of Copies: 2 / Recombinant expression: No
Mass
Theoretical: 11.394426 kDa
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #6: protein, Histone H2A type 2-A
Protein
Name: Histone H2A type 2-A / Number of Copies: 1 / Recombinant expression: No
Mass
Theoretical: 13.994354 kDa
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #7: protein, Histone H2B type 1-C/E/F/G/I
Protein
Name: Histone H2B type 1-C/E/F/G/I / Number of Copies: 2 / Recombinant expression: No
Mass
Theoretical: 13.937213 kDa
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #8: protein, Histone H2A type 2-A
Protein
Name: Histone H2A type 2-A / Number of Copies: 1 / Recombinant expression: No
Mass
Theoretical: 14.125549 kDa
Source
Species: Homo sapiens (human)
Source (engineered)
Expression System: Escherichia coli (E. coli)
+
Component #9: nucleic-acid, DNA (145-MER)
nucleic acid
Name: DNA (145-MER) / Class: DNA / Structure: OTHER / Synthetic: No Sequence: (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT) (DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DT)(DC) ...Sequence:
Name: DNA (145-MER) / Class: DNA / Structure: OTHER / Synthetic: No Sequence: (DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DA)(DT) (DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA) (DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA) (DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA)(DT)(DC)(DC) ...Sequence:
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