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- EMDB-10154: Class2C : CENP-A nucleosome in complex with CENP-C central region -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-10154 | |||||||||
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Title | Class2C : CENP-A nucleosome in complex with CENP-C central region | |||||||||
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![]() | CENP-A nucleosome in complex with CENP-C central region: nucleosome ![]() ![]() ![]() ![]() ![]() | |||||||||
Function / homology | ![]() monopolar spindle attachment to meiosis I kinetochore / condensed chromosome inner kinetochore / centromeric DNA binding / attachment of mitotic spindle microtubules to kinetochore / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Ali-Ahmad A / Bilokapic S / Schafer IB / Halic M / Sekulic N | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: CENP-C unwraps the human CENP-A nucleosome through the H2A C-terminal tail. Authors: Ahmad Ali-Ahmad / Silvija Bilokapić / Ingmar B Schäfer / Mario Halić / Nikolina Sekulić / ![]() ![]() ![]() Abstract: Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is ...Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. CENP-C is considered to be a central organizer of the CCAN. We provide new molecular insights into the structure of human CENP-A nucleosomes, in isolation and in complex with the CENP-C central region (CENP-C ), the main CENP-A binding module of human CENP-C. We establish that the short αN helix of CENP-A promotes DNA flexibility at the nucleosome ends, independently of the sequence it wraps. Furthermore, we show that, in vitro, two regions of human CENP-C (CENP-C and CENP-C ) both bind exclusively to the CENP-A nucleosome. We find CENP-C to bind with high affinity due to an extended hydrophobic area made up of CENP-A and CENP-A . Importantly, we identify two key conformational changes within the CENP-A nucleosome upon CENP-C binding. First, the loose DNA wrapping of CENP-A nucleosomes is further exacerbated, through destabilization of the H2A C-terminal tail. Second, CENP-C rigidifies the N-terminal tail of H4 in the conformation favoring H4 monomethylation, essential for a functional centromere. | |||||||||
Validation Report | PDB-ID: 6sef![]() ![]() ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
Header (meta data in XML format) |
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Images |
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Archive directory |
-Related structure data
Related structure data | ![]() 6sefCM ![]() 6se0C ![]() 6se6C ![]() 6seeC ![]() 6segC C: citing same article ( M: atomic model generated by this map |
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Similar-shape strucutres |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire CENP-A nucleosome in complex with CENP-C central region
+Component #1: protein, CENP-A nucleosome in complex with CENP-C central region
+Component #2: protein, nucleosome
+Component #3: protein, DNA
+Component #4: protein, Histone H3-like centromeric protein A
+Component #5: protein, Histone H4
+Component #6: protein, Histone H2A type 2-A
+Component #7: protein, Histone H2B type 1-C/E/F/G/I
+Component #8: nucleic-acid, DNA (145-MER)
+Component #9: nucleic-acid, DNA (145-MER)
+Component #10: protein, Centromere protein C
-Experimental details
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Sample preparation
Specimen | Specimen state: Particle / Method: ![]() |
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Sample solution | Specimen conc.: 1.2 mg/mL / pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: OTHER |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
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Image processing
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3D reconstruction | Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF |
-Atomic model buiding
Output model |
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