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- EMDB-0233: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -

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Basic information

Entry
Database: EMDB / ID: EMD-0233
TitleCryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit
Map datamap of the head of the T. brucei mitoribosome small subunit
Sample
  • Complex: head of the T. brucei mitoribosome small subunit
    • Protein or peptide: x 27 types
    • RNA: x 1 types
  • Ligand: x 5 types
Function / homology
Function and homology information


mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast / mRNA stabilization / nuclear lumen / ciliary plasm / mitochondrial small ribosomal subunit / structural constituent of ribosome / translation ...mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast / mRNA stabilization / nuclear lumen / ciliary plasm / mitochondrial small ribosomal subunit / structural constituent of ribosome / translation / mitochondrion / RNA binding / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Rhodanese-like domain / Ribosomal protein S18 superfamily / Ribosomal protein S9 ...Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Rhodanese-like domain / Ribosomal protein S18 superfamily / Ribosomal protein S9 / Ribosomal protein S9/S16 / Ribosomal protein S11 superfamily / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S5 domain 2-type fold
Similarity search - Domain/homology
Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein ...Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S9 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / LysM domain-containing protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Uncharacterized protein / Small ribosomal subunit protein mS29 / Rhodanese domain-containing protein / Uncharacterized protein
Similarity search - Component
Biological speciesTrypanosoma brucei brucei (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.08 Å
AuthorsRamrath DJF / Niemann M / Leibundgut M / Bieri P / Prange C / Horn EK / Leitner A / Boehringer A / Schneider A / Ban N
CitationJournal: Science / Year: 2018
Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
History
DepositionAug 31, 2018-
Header (metadata) releaseSep 26, 2018-
Map releaseSep 26, 2018-
UpdateNov 7, 2018-
Current statusNov 7, 2018Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6hiz
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0233.png

Downloads & links

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Map

FileDownload / File: emd_0233.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmap of the head of the T. brucei mitoribosome small subunit
Voxel sizeX=Y=Z: 1.39 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.15
Minimum - Maximum-0.2582923 - 0.5873829
Average (Standard dev.)0.0010537558 (±0.015823135)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 444.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z444.800444.800444.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2580.5870.001

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Supplemental data

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Half map: half map (even) of the head of the...

Fileemd_0233_half_map_1.map
Annotationhalf map (even) of the head of the T. brucei mitoribosome small subunit
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map (odd) of the head of the...

Fileemd_0233_half_map_2.map
Annotationhalf map (odd) of the head of the T. brucei mitoribosome small subunit
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : head of the T. brucei mitoribosome small subunit

EntireName: head of the T. brucei mitoribosome small subunit
Components
  • Complex: head of the T. brucei mitoribosome small subunit
    • Protein or peptide: mS48
    • Protein or peptide: mS59
    • Protein or peptide: mS49
    • Protein or peptide: mS50
    • Protein or peptide: mS52
    • Protein or peptide: mS53
    • Protein or peptide: mS54
    • Protein or peptide: mS55
    • Protein or peptide: mS57
    • Protein or peptide: mS58
    • Protein or peptide: mS67
    • Protein or peptide: mS69
    • Protein or peptide: mS70
    • Protein or peptide: mS71
    • Protein or peptide: mS72
    • Protein or peptide: uS3m
    • Protein or peptide: uS9m
    • Protein or peptide: uS10m
    • Protein or peptide: uS11m
    • Protein or peptide: uS14m
    • Protein or peptide: uS18m
    • Protein or peptide: uS19m
    • Protein or peptide: mS29
    • Protein or peptide: mS33
    • Protein or peptide: mS35
    • RNA: RNA (143-MER)
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
  • Ligand: SPERMIDINE
  • Ligand: URIDINE 5'-TRIPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
  • Ligand: MAGNESIUM ION
  • Ligand: water

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Supramolecule #1: head of the T. brucei mitoribosome small subunit

SupramoleculeName: head of the T. brucei mitoribosome small subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#28
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Molecular weightExperimental: 1.25 MDa

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Macromolecule #1: mS48

MacromoleculeName: mS48 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 201.811188 KDa
SequenceString: MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL ...String:
MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL YQAGAHGAQL SNPRSPFTAK VLGDELMQVR DIKSVEHCKA WFDRLQYLIN LHYEAVGDIG EFKSRHTRHV HE FFVAFHD ALSSLDFRDT YLFDQFKAVR PPELSDLFGI FLEMEANYVH EDYCPRCSLP YSTTRYCGEG DVDTPFRKHR GRW APHQKW GREWYDVVAR RAEALWYRAT EDPYFGTPQH TQRQAEALLK VYVQAKQRGK AIDFMNKLRG SVEYLTGDIT ITTV MQESY DALLDTTPHP HLLTNGFTLE SDAAKYTGEV NKAPLSPLQF RIDMEMNKYR RQQKEEGVVR VPPALWRLDT SAIVP YKVD SKTRRIVNWR EVKEGIEKSF LSVGLPKEAY TGNEWREMLY LHDVIANREA KAAVLEQQQK LDREKLKARE RASLKG EKS SNSASNGIGI IFPDKDGYQV FFVDEASLRP FGIGASGTLF KAVARVYPSP AAVPYDDPVH GKQFLIDTTD ETCHLFG GF EHGDRLLLRA KSSGGDVDGG NNSNCRNTGS FTDEEEEVIV MGVSTEGSDA ERMLCAMHVD TGKQRERGIV FLGTDCID I RERWESVRYA ASAYVKGRVT LLESQRTARE EFMGQVVGIR DGVLFVQWRL LKGGGSVLDR SVAVPIGDST QVRELFVET KVLGVEPLQS PPSWRTPFRN DFAEERLKEL QRAPFKREKY VSLIQGKYTP KVKKFGYTQH TTVDDFETKE YKDRLLSKQF FQNPQAFEV VPDRHERSVQ FGGKWEYQRT HGLPTVDRNE LENGWSEVEP ISDGEMKVIE QALRDISGPR PGNFVKPPSK T KSLQLSES WWEPLGYGWE QHNEEQKALC DVTEQRLIDG SNLPFGGKLP PFGTSYGMGE RMRAIIEDYS KGFGLGPHGH SP THDTIHY NTLNAEGERV RDLGYTDALG RLFSEKLGDR DVHQWAVESC ADGEADVRQL LLSLHEWRER GRPPSLLLAN VLS KYLEEE IAAFNKGIPE NAPKLQLETS DGTLAHSGSG GSRSGTMWAD VDPTTFALHQ ASQSTRSTRC DEPFILHLVK RAKL GECVT NFTDTAYIAH LESSVINEFH LALTKLVGKG ISPTLLAQKT GQLHRGSVRV GGNVVPFVKS RELSRLLERM GLTSE SIAV ITRGLANCPE QESVGDDFAV PVSLILSWDG PGSSGGSGSG DRRLNTTAAR NEQQRKGSAA LSSAIRQLSQ HQKQRG SAS GRGWQNEDKM VVHVLEELSL RNDGLVMDIQ YAIRENKKNR RLRWEFVTTL LPVFGGNEAK VEQLYSDYCD GKYVPNI TV AVEAFIAFLH NATQHPETYS ASDYFDIDDG KSSTASGTGD QPSAGQYTTL KLLDPLEGPF VFDNVKVEYI QTVERFRR H GIRAGPVMAP ATGFIAANCK SLNYFTRRQE EVVYVTNDSD QGLRRSLENS AYHKTIAANP ALQYLLKARR GAALVETFN RFFYRTMPML NFYQNVLKHY SETIQPLRQA AKSSTRGLAR ALESERSAAM EEFRRNSERY WRNIIEGRSV EQVVLANGES NRRTVGGGA GEQLQKPQGN RDAVRTNFTG EATDGSRKGQ RQHQQPPLSQ QQQHSRGEGE RSAVHTSPRQ GGSRGMADLL S KLNTPKTG SGVKGSTAKN TGNNRRGPKS

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Macromolecule #2: mS59

MacromoleculeName: mS59 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 35.395676 KDa
SequenceString: MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES ...String:
MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES AVRSPAYREI AKRQLEGGAA TRLAGISAAA DVMRRMGLTR RPMSSYFELY QRIVERSEAM TPWGFPPLFQ FE ERLSLEP RLKFFSRASQ QSLERRRRGH VMTPHTTLHG RRIFWIPPTW NRAGRFLGPH VTLYPGMTPD

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Macromolecule #3: mS49

MacromoleculeName: mS49 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 137.234125 KDa
SequenceString: MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE ...String:
MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE LLRSRAAEYQ VANTVHESES VHPIQQLYNS GGTSRSALKQ QAVADRYSIA EQHSPLPLTT GVDRDALDEA QR TKDRILN DSFTAENLLI THGLREKEKH DFTILQRTVR IPFQGYDMDR FLAQQKGTPY GAQQLPPNVV PSSMEEAQRT LRG SSATAT PLVDAVAQKV YARNTVVDRP AIGEQLTEQI INIMRASRTT AEQQREEERA QRFGLGRQGA LVQDGGPDQR TLKK HTNDE RIVDAMLFQQ NAYRKTPTDE HWNPYIRRST ENGVGHLLQN KFDIMRREDR LSKGEQDLTE RNTIHYGVPI QQIVD EFVF RHRNARGERP LDYFKPFPNF RALRLNRMYR DVEGFSLMKQ RPEFLEWELF TRYRQHHQQR RRLALLHGLE PVANET AQE RDTRRHRLDE ICERTPFDER EMRVNDDEMR VSVETLRSWF GVYMLPSPTV VNAVLGGSAS VNLHLYHLAD EMGTADT RE HVLSSRYLNR LLLLESYQNR VGRGFMNHVV GRAPEPVVPH EQPQEVLRHF SAEERAMYEQ HVKEQTSRQL GEWERAMK R RRWLTDHQQY GHVVSHGLET SVVDLSHTET GAVLTVSTKA YEQEIEAVRM KTNATIKVDG MVYNLLPNSE RRVVPLTVQ LDSGEKIDMT SEDFDRCELE AFPRNLNHAL NYGIANYAYN RGNYVETQDS IWEEQTASGQ EGWSPATHAD GLREGLPVRA RRPIFSSSA EQRIAGGPQR AVIIQYHHQP FFNPEPRLVK VAFQCDGTIM EVPISDVMIW QRRYHGPERT VGDESRRYNP A AMRRYVDV TDPFNEKTSN TEHFLDKYEP KRNADTVADK YRTTKQITEI DKWTRYDSAR ADNYRPLSIS HRRDYIRMGY IP RYTPWEW IAIQEADQPL IAEQIRQDNI GTSYFFSLNR YWRYKASPHG YIRHFENEVR DLLQYVDGVT PWKQAQKIRT YWE VRSHHP MPQFNRPEVA MHRNTVGLLP AHMWETDKKT GKVKSVKDSV RDYQTKTPYP KWVQL

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Macromolecule #4: mS50

MacromoleculeName: mS50 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 131.71925 KDa
SequenceString: MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF ...String:
MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF DDESDRPETR WSQDTFFREC AYLSEKFLKE KVSNMQQFEA ALKRASPEAY LAFFDAFQQQ TQTQIPLPSP SV WHYEGER RKQWAEKFIS ISHKAQAFFK DVLSEDVKKY QEVPGKLLQK VKPVLADVGK ILVKRHERWL KGRVWTSLTE EER EAYCMK EVKRQQMQVE DGEFDPMMED DVDDTELEEW QREHDAIMKL MNSPIDGLHF TTLELWLHTM RCEELETEHI YTSA RVRAI QVAARKKLYD TTSYEEVIQA VVESIARGTL DLGAGVLRPH FNEVWCQLNY AKFGSSTITQ HTTTSRRQLL FFHAG SLKD IAATATLYYA TKPLSNSLDY ASPYKYRRSL ITLCSNYGVE TAYTTQRPLL RSAANLARAE DLIHAVVTAA AQPFGE RRR AATRDLHMEF QRLAVPVERV IVANPVSALL ESGADPDEKP VEGEKVNMWP LGAKRVVLYK WSAPNVEKLK AMESDAA SA VSGSSLTAKR LREIQELKRR GFLEVSLWRR VTAQERKQRN EIVEAKKKQV EEVVRTVPSL AHLHQYATSL YSRIEERV A FPTETSTVTE TTNMKEETNK PLEDSEWEFA VLLDDRVLLN KEESVELYLP YRDANGELLA QGEYRALVRA FDLEANPNL HPAYCSVGYS ESFQVFDALP QLIAQFFRVK DATAEAAGVT HIPAADFTPF CAFLRDAGLD VPLRCEFEAG QAVTTDGDVY MDYFLQLLR GEAFHQSHAQ AGLTEAQRAI EPLCRAHWVV HHPGADESEW ATARRSVLDH AMQHEREWWF PNEMLDVKDV V TGSTNGLT PQMYPAAVRY GVELCTVLTA EGKFVDERGS GLSARCVVNG TGAAESVVFD TANCNGTNTT SVEDALRVAH GA LRSAQDR HNTLAAFRLG PLSKQSQVLL FCGVNAYEFG GKYARTYAYA FEKAKKELEA TAASGFMAPS LSHEDTERLS DQP TTSPSV DRFASTTHPE QRKAQFVPRV GPGSTPLEDP AADQKSEWS

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Macromolecule #5: mS52

MacromoleculeName: mS52 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 85.012258 KDa
SequenceString: MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ ...String:
MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ SILDEVARAQ GMLDLFERQP HFPAIHIDRC SRFHLVELFK EMVLERSLDS NMIWEKALLY RAILSERKPS YP TSFHYIF TAVEDTVFAP TISTPMEMAG SGGEGHDRSS HPLAAKCPTL EAYYYYVYLV KKYYIDNAVE AHVVLRCHRE PNA ADLLFS NPPPKDDTEI MKAVELLRNA DIQRGVAAAA AVSDPTLPPG GEGSVIGNSD NKKNSEKTSE GSRGRPARPP VLPG AYPPI DMLWRCEENL PLLKVLLFGE FNLIVSENPF VKFPSAHGFL TRPYSTDSSR TLADGMSLAN VMAEKRGHLL PSLPR NTAT SIDARAQDIR RLQQKHHRDD IVSFQKLLRT THAEDSPSAF SSYSDWSYFN PRAVRAEERD RLTRKAVEAL KLYDSA TND IYRHSFEDVQ ACHTQRVTER DRTMPPYLPT LPHFVAIIKK DPHISFLLHI GLPDRNSSEE GSAKHKELEK RIYYLAR AL YHTALEYHNE TVRRVNRQKV NVAASLLDNF VEQEWTTILR DKHDVTDVTK TLNDTQNDKK QLARRLGRYM LFANRSLD D TGFPTDARAD DYTRWMAPPS VGKVSL

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Macromolecule #6: mS53

MacromoleculeName: mS53 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 75.338914 KDa
SequenceString: MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL ...String:
MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQVDN YFRRSVKLNR DGTLPTDFVH EAPLMRKIIR LAHRGHLKAA CEEYRRVTTV PPVEVYRALT AC CVPGAKL ADAVSIFEDG DSKLFYVSRD GEVLHNLMRC AIAARHRARI MWVYNVMRGR FYENVVVRAE VDLIWRYRIA MIA LEYLLD HECAEEAAAI YSYLVEEELL RCDVHVRVGL HMREAIAAGK PITLNNDVMN ATSLVRDATA VAPEVARELQ RRHA QTLQN NAVEAVGAEN VREGSAPWSI LGPLTAIGPT AEDTMVWLQQ HYGDVDVMSI MRWARFRKGK DLMAKDRPQY LARAA AWIE LLSKRNREME EVPLTYMRKS KPLVLDTNSN VRVAWQTPLM RSGGPPRLLA REEGYVFHHS NSSRFVEETY RHPGES LQS RYLALQPLHT EVSAKEDFQR LYYQAQKHHK QQERLLPVST AAIPSRIVHH SVMSALHGVS GKGEPANRSL FT(UNK) NKSNDS HSNTGVASGT SACTDGAGSA TPEF

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Macromolecule #7: mS54

MacromoleculeName: mS54 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 72.046031 KDa
SequenceString: MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY ...String:
MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY NNLAMEEVER LAPHRADRLL KEQMKRFRGN LQQLGSGLRP TVYCRYLHTT HTAAVLLEEV HNFLWELVPN DH PAMEIPA LQLRVPFVAS VLRRPSVNPG VSLASVSRAE FGDTDVCAVF LAAAERMVDA DFDDQRPVSE RRLFLSLLTM ISY SGVLYT SDLMAQLMEM VKYSNNDETR DSDAQRVLRY ALRGSSAAQD SASRTLWHSV EKVADCRVVG RYIGARNPWN PIRV CFDEQ GVFKAYPIST TTTTREVSPP EGNGAVTQEQ RASCVEGRTL EALNMRWDDV RRLIECTGVL VTPPSERCPQ QQKME VFTG MAVYLRTVAT GRRYEGGEDV LSDGAVATSS CEQRRRGTLF AEGYDFDVWV RLFSLVQEVR HDMEKFMADH TLQCVE PEF ECWEALLVTL RCALDFCVVQ MQGGGARGTE REVVERLFRD VVALREELIE ESRTRFGGRM RVLWLQEA

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Macromolecule #8: mS55

MacromoleculeName: mS55 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 66.67668 KDa
SequenceString: MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA ...String:
MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA YRWFDLALTS LAVDCGGRTQ PSHFVPYLPQ GTAAASHITN ALMRTLLTCG YTHVAAIPDS VYDRMGSMGI SP TISTYEL VMLALSLQGN MVEAESILSF LRSHHSEHIT VESFNALLLG HREARQFDCC DAIWQELVDR RWPRASPLTA ELY LRSIMD HANTPTSEPL QSFANINVVE KKKVPLVLAQ MDELGVPRTH LSRVLMDEVE DSLRKFQTYR SRFYEWGRAV KQFD FIEFR RRNGWLYDLH LMKCTTKQVG PLRDFNDPDA VQGAVATAEI PAFFNERPAW ERPPLEETLY VTTNKERYDD VRGGD IYYD DTRGLHDRSP TWMNEVPETR YDRLYGVNHP DIAKIGIRRH LNVEYVNRKE VVERDAALMK KTLSSGRRLR HRVESS RTH RNAGSLSGIS STAGGGSR

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Macromolecule #9: mS57

MacromoleculeName: mS57 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 45.956293 KDa
SequenceString: MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY ...String:
MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY CNKTDNSGWR ALFDRCLKDG WNYTPLFDTA QWSFMLKSIG RMGDEDGVRA VLEEMLDVQA DLDRVEARSV VI ALNAVTN ADVYEFVKKY LFNFGERKVK FLRTTYSDLR GHGAGKLRIP LKENDNMYYH VCWHSSIRSP RQFSPRQLYF DYT PSTLGS SSHNPNAKID DIVKDKIEKW KAEGLLPEDY VHEDRVYDRS AAFKNVARQE KWKKMPKILK SKRMGYTGDP

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Macromolecule #10: mS58

MacromoleculeName: mS58 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 35.62391 KDa
SequenceString: MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGSLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP ...String:
MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGSLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP WMQLVQHGKK EEAAALLKEF EQKGDAAEAT PTRVKDDRVK GTRTELSNRE QPPLASTLKA EDSNPATIPI GS VHPLFTP ALVQIEEAGG GSVSNEAVCR AQLWELSRDE QLFWERVELI RKGQVASIGS SHKRGYADEA AFAKDDTEEK VQL RFTQ

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Macromolecule #11: mS67

MacromoleculeName: mS67 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 30.096051 KDa
SequenceString: MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE ...String:
MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE LFGESYSDMN VSDVLSPWKG PFPQSGVFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL EKARHPEEGP RQ NMPFGLQ

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Macromolecule #12: mS69

MacromoleculeName: mS69 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 21.589732 KDa
SequenceString:
MRRVRDGLFL SHPSSALQCS VAVVSTGEFD HPPFQFRQRH TFNTTPLHDA NRFGGRTAYL REIGPVNIKK QGRRFKKDPR TVQFNVDVW CAQQTLRKRW KQRDWEVIEI PFRLVPREQQ RVIPELYTDI PQMTDPARND FSNIRNKVYD REELQGVLFP A AGAMLYPP LQRVDKQAMT LDKYL

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Macromolecule #13: mS70

MacromoleculeName: mS70 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 21.247398 KDa
SequenceString:
MLRFTHRALT ATPERFSVLG TTHPKPKRTG FGRNNKMRSK PSDNVAWYDK GPVEWLPRPV RLTYDHLDQL QQWTMRATLD GRTEEFNRI RDLHREWSQH PLMPVLGDVE PKFPLNLFKQ NHRAKKRFLV RWHKANTPAN WLWMPRGPTV VTPLHRTNPT Q YPENWKQM VQRKSGTGTP S

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Macromolecule #14: mS71

MacromoleculeName: mS71 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 20.093227 KDa
SequenceString:
MFHRAFVSSS DLTGCTIALS SVCTQKRYWA KPKKRPKVGQ GFHEKAQKWR EEYLLDRHRA LADSLRAYVE FSTSKRVEPW DARFKPFDR IEKDGVYVLM RYMMEEKLQL CNYHHRPVKR LFCNIGLMGP QITTKARWKP YRFATNPAGT SKAERMYQRD K TVYTHGHN D

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Macromolecule #15: mS72

MacromoleculeName: mS72 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 19.317293 KDa
SequenceString:
MLRSTLTSLN TFLTSSVATP PISVIRTGPK WWADPERMVR QKLMYFTLGV DQLPLRRTAV IQRDLQRFHM CKPPPRVGDS TGYKRSRAA QLNTWYRRIQ YQEYHMQHLF TRHVWGLLRV YPGNTTKIQG KADDGYVGYD SVPFHRYNRA PLPFPARELY E RRK

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Macromolecule #16: uS3m

MacromoleculeName: uS3m / type: protein_or_peptide / ID: 16 / Details: Mitochondrial gene uS3m (MURF-5) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 9.07094 KDa
SequenceString:
MFLIHFVHYK TILQKYTFKF KHIFLSIDKY NSLFFNISGI LIWLNIIHIN IILIKYSFFI LINNFEYLII LIST

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Macromolecule #17: uS9m

MacromoleculeName: uS9m / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 50.128926 KDa
SequenceString: MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE ...String:
MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF A(UNK)KVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEK YHFTALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALVKLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

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Macromolecule #18: uS10m

MacromoleculeName: uS10m / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 94.351039 KDa
SequenceString: MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR ...String:
MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR EHHQHARILS PLEVQRDVGR LEPIPSYAGK INADRSVFPF QWKTEDWYEY EVAKVRNRRF VFENTEEDGI RG SEVTYKI VLEGFWDHHV MKLAEDVCMF LKDVGRQIVE EKLVAVRRLL QGGAVDPELL AAFNCARAGP FGGLDEYDKE EVA NFLRSD LRRLEEQCLS VINRCNVPVP GATNIYDPHT SWPHVEKLEP WVRMAEFWTS SSDTSFTELE MSTAHYEFRK FFRV IICKL PFQSTEFEKR MYDIRHWLHR QTSCEFHTIY RRNVIHDSAV FPTEHDPATP TTHEHHRMFS FALDWQSAPV NRLST DTVH EGESWDAVAQ RLGCSVGELK DANAERETIE AGVVINVPVT ATRRLTSFGA TPLVLPLKTT SAKDGERIRT WEEAAA ILD CTVEELQQCN GHAALTYQKK ESEAGEFDSS ETELVAPLSC WTSTSESEFS PVERVHANDT LVAIARRLQC SEEALRA VN DGITDVSGLD FVRVPPEARR PRRLVEPQLR PQAATDALLA RTIAEEETFK LKSIPHLPQN AERFPHEYHT PTSRFPPT P SETPATQDWM AYTAKYLDKQ FTISAEPAPV YNVNKLWPMQ QIPGKVDQTP FEEDQTWLLH SIPVQQLEMH HHEKDLQDL PFINHEQFPR SLEWNAP

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Macromolecule #19: uS11m

MacromoleculeName: uS11m / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 37.92591 KDa
SequenceString: MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE ...String:
MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRS E GSTDNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLN GPKMPRGVFK

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Macromolecule #20: uS14m

MacromoleculeName: uS14m / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 19.900014 KDa
SequenceString:
MFCFSRGLWM RHIGQDVPKR HTHFVLESRL MYEKSFRDCW LHSVCRAISQ LDEPLSKTVV GTHQKMLQRK VTCFQYNQYG LFKTPYYRL ANVDRYHAVQ GVAGTREWVP YVNVSYWTMN KMVRGGNLLV HRVHYTGWGT DSHLKKGGWE HRWNKVLQRN V LQYSRI

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Macromolecule #21: uS18m

MacromoleculeName: uS18m / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 36.988691 KDa
SequenceString: MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI ...String:
MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKRTVEHF AYNWVQTDRI RRYFRELEAV HTSGSASARG SG GGTTAEH KQQDQFYSPE NQPISLHRNN ISYMEDVGRS VKNPTVPGLM STKGMKKKFH NLYSSTSTKR MGFSNPTLGI KKV

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Macromolecule #22: uS19m

MacromoleculeName: uS19m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 28.364604 KDa
SequenceString: MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG ...String:
MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG KFFVSKRQVY TLQHATSGAK VRVKSFPSIF EFNSPSRWNI GKEMNTLTKP RMDLIDEQML TKKQRLDYVR AG LLPK

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Macromolecule #23: mS29

MacromoleculeName: mS29 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 57.048836 KDa
SequenceString: MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS ...String:
MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS RNIVTLYVPD AKVWTHGEWC WPSTILPGFF DAPDAARSFL KYFAVANRAT LTSWKLRCTP KDLPTEQGER QP QNLYELC EWGHRAVAPA SIDRQSVCVK FLMDELSEEK KLPVVIVVDG WNLFSHETHF RYPHPDFLRG LASFNESSTD IDL YPQELP RIPASRLSFV RGLNKMILSG DDPNKFFITC TTRDFKPFDG ISGFPNVETD RFANSLDEYA PYDPEKDSHF HPIQ IGNFD EYEYRSFLRF LINSGELAGL GWGPLWHASS DFERKLYKIG FLSGRNPQGV VDHYHQELVW RYDYQRTRQK QYLLK RRME GMSRGASSGA VGVR

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Macromolecule #24: mS33

MacromoleculeName: mS33 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 21.244104 KDa
SequenceString:
MVLRRWFPLL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMRDMRPGY GQNLPDYIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFKVLCS S GAKNPPSG WEPIPDATEE EED

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Macromolecule #25: mS35

MacromoleculeName: mS35 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 98.377773 KDa
SequenceString: MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE ...String:
MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE KVVIPKLTSD EEDRRKLVSL WSETTLQAEA AVTKFLFQRG SFESMLHRII TDALEKMSTL ALGGQEGNLA LE ALKRQTL FKRNDYIQKR LIDVVSNSAY LGYGDSVWQV FFAAVEANEE NLLSDRATTD AIRAAWEGVM REDVVRLPDV TGV VALYLT LVCIRESGRL VPEELKELSS GLEDGVRPGV RKLQQYPLIF LHPTVKRRFV VKAVAEILHN SSSNAFSNML RENG LHDTA REVALCEAMN RNKELAEGDV GDAVGRFVSK GEVKTLLSSL VSGTDAVVRD AVAGIFGIGT TITIDWDAVM QNVDW SNNW QRLATALLSN SAVLSAIVKL VKNAIGAKGM SKHLFTDEYA DQLQLILDAR EERAASRKQR IENIAQELSS FERVDL SCD LLRKLGVDMT ELDTAAAATR NMNVVQRPCI EDGLLSLVLE AVTKRHPNWV KAGVIQTTLK DPFDALRWMM HIFIRLS YV PHAGAATIAR LSRRRIGPIG LEPHQFNVPA ELGFVEQYDN LQYKRYDWQG WYQRMLDVHN RNVSLRCRIC DLQRLDGN G VQFVDMQTER RLRILAQHRV GMGVLKLDAD KYEDQADNVT FGTTKLSELL ADARKAQLGE EYWPSVELKV RKPSGQSKA HYSLIDNERI EKRSRELYEK YRDAKKRSLF VTPMETWLEV KGMQVRKSVD NADEDGYTLD ALQDMMDGDD GDKV

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Macromolecule #27: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 443.539 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #28: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 613.749 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #26: RNA (143-MER)

MacromoleculeName: RNA (143-MER) / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 195.379688 KDa
SequenceString: UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA ...String:
UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG CUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGUUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAUAAUAAAA AUAACGUGCA GUAAUUAAUA UAUUUAUAAA AAUAUAUU

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Macromolecule #29: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 29 / Number of copies: 2 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

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Macromolecule #30: URIDINE 5'-TRIPHOSPHATE

MacromoleculeName: URIDINE 5'-TRIPHOSPHATE / type: ligand / ID: 30 / Number of copies: 1 / Formula: UTP
Molecular weightTheoretical: 484.141 Da
Chemical component information

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

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Macromolecule #31: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 31 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

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Macromolecule #32: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 3 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #33: water

MacromoleculeName: water / type: ligand / ID: 33 / Number of copies: 3 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND
Startup modelType of model: OTHER / Details: initial model from 2D class averages in EMAN
Initial angle assignmentType: OTHER
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.08 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 101308

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Atomic model buiding 1

DetailsWe used Coot and O for initial model building and refined the structure using Phenix
RefinementProtocol: AB INITIO MODEL / Overall B value: 45
Output model

PDB-6hiz:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit

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About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

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