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Structure paper

TitleTime-resolved cryo-EM of G-protein activation by a GPCR.
Journal, issue, pagesNature, Vol. 629, Issue 8014, Page 1182-1191, Year 2024
Publish dateMar 13, 2024
AuthorsMakaía M Papasergi-Scott / Guillermo Pérez-Hernández / Hossein Batebi / Yang Gao / Gözde Eskici / Alpay B Seven / Ouliana Panova / Daniel Hilger / Marina Casiraghi / Feng He / Luis Maul / Peter Gmeiner / Brian K Kobilka / Peter W Hildebrand / Georgios Skiniotis /
PubMed AbstractG-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by stimulating guanine nucleotide exchange in the Gα subunit. To visualize this mechanism, we developed a time-resolved cryo-EM ...G-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by stimulating guanine nucleotide exchange in the Gα subunit. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines the progression of ensembles of pre-steady-state intermediates of a GPCR-G-protein complex. By monitoring the transitions of the stimulatory G protein in complex with the β-adrenergic receptor at short sequential time points after GTP addition, we identified the conformational trajectory underlying G-protein activation and functional dissociation from the receptor. Twenty structures generated from sequential overlapping particle subsets along this trajectory, compared to control structures, provide a high-resolution description of the order of main events driving G-protein activation in response to GTP binding. Structural changes propagate from the nucleotide-binding pocket and extend through the GTPase domain, enacting alterations to Gα switch regions and the α5 helix that weaken the G-protein-receptor interface. Molecular dynamics simulations with late structures in the cryo-EM trajectory support that enhanced ordering of GTP on closure of the α-helical domain against the nucleotide-bound Ras-homology domain correlates with α5 helix destabilization and eventual dissociation of the G protein from the GPCR. These findings also highlight the potential of time-resolved cryo-EM as a tool for mechanistic dissection of GPCR signalling events.
External linksNature / PubMed:38480881
MethodsEM (single particle)
Resolution2.9 - 4.2 Å
Structure data

EMDB-29951, PDB-8gdz:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#1 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-29952, PDB-8ge1:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#2 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-29953, PDB-8ge2:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#3 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29954, PDB-8ge3:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#4 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29955, PDB-8ge4:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#5 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29956, PDB-8ge5:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#6 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-29958, PDB-8ge6:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#7 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-29959, PDB-8ge7:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#8 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-29960, PDB-8ge8:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#9 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-29961, PDB-8ge9:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#10 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-29962, PDB-8gea:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#11 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-29964, PDB-8geb:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#12 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-29965, PDB-8gec:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#13 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-29966, PDB-8ged:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#14 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-29967, PDB-8gee:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#15 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-29968, PDB-8gef:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#16 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-29969, PDB-8geg:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#17 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-29970, PDB-8geh:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#18 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-29971, PDB-8gei:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#19 of 20)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-29972, PDB-8gej:
CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#20 of 20)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-29985, PDB-8gfv:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-29986, PDB-8gfw:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29987, PDB-8gfx:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29988, PDB-8gfy:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29989, PDB-8gfz:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29990, PDB-8gg0:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-29991, PDB-8gg1:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29992, PDB-8gg2:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-29993, PDB-8gg3:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-29994, PDB-8gg4:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-29995, PDB-8gg5:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-29996, PDB-8gg6:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29997, PDB-8gg7:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29998, PDB-8gg8:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29999, PDB-8gg9:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40000, PDB-8gga:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40001, PDB-8ggb:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40002, PDB-8ggc:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40004, PDB-8gge:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40005, PDB-8ggf:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40009, PDB-8ggi:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40010, PDB-8ggj:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40011, PDB-8ggk:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40012, PDB-8ggl:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40013, PDB-8ggm:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40014, PDB-8ggn:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40015, PDB-8ggo:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40016, PDB-8ggp:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40017, PDB-8ggq:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40018, PDB-8ggr:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40019, PDB-8ggs:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40020, PDB-8ggt:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40021, PDB-8ggu:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40022, PDB-8ggv:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40023, PDB-8ggw:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40024, PDB-8ggx:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40025, PDB-8ggy:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-40026, PDB-8ggz:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-40027, PDB-8gh0:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-40028, PDB-8gh1:
Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-40096: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#1 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40097: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#2 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40098: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#3 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40099: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#4 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40100: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#5 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40101: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#6 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-40102: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#7 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40103: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#8 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40104: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#9 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-40105: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#10 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40106: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#11 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40107: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#12 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40108: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#13 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40109: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#14 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-40110: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#15 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40111: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#16 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40112: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#17 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40113: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#18 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40114: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#19 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40115: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 5 sec after GTP addition (#20 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40116: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#1 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40117: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#2 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40118: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#3 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40119: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#4 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40120: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#5 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40121: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#6 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40122: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#7 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40123: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#8 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40124: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#9 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40125: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#10 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40126: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#11 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40127: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#12 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40128: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#13 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40129: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#14 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40130: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#15 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40131: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#16 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40132: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#17 of 20)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-40133: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#18 of 20)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-40134: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#19 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-40135: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 10 sec after GTP addition (#20 of 20)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-40136: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#1 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40137: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#2 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40138: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#3 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40139: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#4 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40140: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#5 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-40141: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#6 of 20)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-40142: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#7 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40143: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#8 of 20)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-40144: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#9 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40145: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#10 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40146: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#11 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40147: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#12 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40148: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#13 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40149: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#14 of 20)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-40150: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#15 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40151: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#16 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40152: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#17 of 20)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-40153: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#18 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-40154: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#19 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-40155: CryoEM structure of beta-2-adrenergic receptor in complex with Gs heterotrimer, 17 sec after GTP addition (#20 of 20)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-40156: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-40157: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40158: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40159: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40160: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40161: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40163: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40164: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40165: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40166: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40167: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-40168: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40169: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40170: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40171: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-40172: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40173: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40174: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40175: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-40176: Locally refined cryoEM structure of G protein heterotrimer from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42408, PDB-8unl:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class A)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-42409, PDB-8unm:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class B)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42410, PDB-8unn:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class C)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42411, PDB-8uno:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class D)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-42412, PDB-8unp:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class E)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-42413, PDB-8unq:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class F)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-42414, PDB-8unr:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class G)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42415, PDB-8uns:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class H)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42416, PDB-8unt:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class I)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42417, PDB-8unu:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class J)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-42418, PDB-8unv:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class K)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-42419, PDB-8unw:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class L)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-42420, PDB-8unx:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class M)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42421, PDB-8uny:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class N)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42422, PDB-8unz:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class O)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-42423, PDB-8uo0:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class P)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-42424, PDB-8uo1:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class Q)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42425, PDB-8uo2:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class R)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42426, PDB-8uo3:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class S)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-42427, PDB-8uo4:
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class T)
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-G1I:
(5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol

ChemComp-HOH:
WATER

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / GPCR / Adrenergic / Receptor / G protein

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