Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of mitochondrial membrane bending by the I-II-III-IV supercomplex.
Journal, issue, pages	Nature, Vol. 615, Issue 7954, Page 934-938, Year 2023
Publish date	Mar 22, 2023
Authors	Alexander Mühleip / Rasmus Kock Flygaard / Rozbeh Baradaran / Outi Haapanen / Thomas Gruhl / Victor Tobiasson / Amandine Maréchal / Vivek Sharma / Alexey Amunts /
PubMed Abstract	Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes ...Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III-IV supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.
External links	Nature / PubMed:36949187 / PubMed Central
Methods	EM (single particle) / EM (subtomogram averaging)
Resolution	2.6 - 28.0 Å
Structure data	15865 8b6f EMDB-15865, PDB-8b6f: Cryo-EM structure of NADH:ubiquinone oxidoreductase (complex-I) from respiratory supercomplex of Tetrahymena thermophila Method: EM (single particle) / Resolution: 2.8 Å 15866 8b6g EMDB-15866, PDB-8b6g: Cryo-EM structure of succinate dehydrogenase complex (complex-II) in respiratory supercomplex of Tetrahymena thermophila Method: EM (single particle) / Resolution: 3.0 Å 15867 8b6h EMDB-15867, PDB-8b6h: Cryo-EM structure of cytochrome c oxidase dimer (complex IV) from respiratory supercomplex of Tetrahymena thermophila Method: EM (single particle) / Resolution: 2.6 Å 15868 8b6j EMDB-15868, PDB-8b6j: Cryo-EM structure of cytochrome bc1 complex (complex-III) from respiratory supercomplex of Tetrahymena thermophila Method: EM (single particle) / Resolution: 2.8 Å EMDB-15900: Subtomogram average of the respiratory supercomplex from Tetrahymena thermophila mitochondria Method: EM (subtomogram averaging) / Resolution: 28.0 Å
Chemicals	ChemComp-CDL: CARDIOLIPIN / phospholipidYM ChemComp-UDP: URIDINE-5'-DIPHOSPHATE / UDPYM ChemComp-MG: Unknown entry ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM ChemComp-LPP: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipidYM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry ChemComp-FAD: FLAVIN-ADENINE DINUCLEOTIDE / FADYM ChemComp-F3S: FE3-S4 CLUSTER ChemComp-HEC: HEME C ChemComp-UQ8: Ubiquinone-8 ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-K: Unknown entry ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM
Source	tetrahymena thermophila (eukaryote) tetrahymena thermophila sb210 (eukaryote)
Keywords	ELECTRON TRANSPORT / Ciliate / mitochondrial / complex-I / supercomplex / Complex-II / cytochrome bc1