EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of mitochondrial membrane bending by the I-II-III-IV supercomplex.
ジャーナル・号・ページ	Nature, Vol. 615, Issue 7954, Page 934-938, Year 2023
掲載日	2023年3月22日
著者	Alexander Mühleip / Rasmus Kock Flygaard / Rozbeh Baradaran / Outi Haapanen / Thomas Gruhl / Victor Tobiasson / Amandine Maréchal / Vivek Sharma / Alexey Amunts /
PubMed 要旨	Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes ...Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III-IV supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.
リンク	Nature / PubMed:36949187 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.6 - 28.0 Å
構造データ	15865 8b6f EMDB-15865, PDB-8b6f: Cryo-EM structure of NADH:ubiquinone oxidoreductase (complex-I) from respiratory supercomplex of Tetrahymena thermophila 手法: EM (単粒子) / 解像度: 2.8 Å 15866 8b6g EMDB-15866, PDB-8b6g: Cryo-EM structure of succinate dehydrogenase complex (complex-II) in respiratory supercomplex of Tetrahymena thermophila 手法: EM (単粒子) / 解像度: 3.0 Å 15867 8b6h EMDB-15867, PDB-8b6h: Cryo-EM structure of cytochrome c oxidase dimer (complex IV) from respiratory supercomplex of Tetrahymena thermophila 手法: EM (単粒子) / 解像度: 2.6 Å 15868 8b6j EMDB-15868, PDB-8b6j: Cryo-EM structure of cytochrome bc1 complex (complex-III) from respiratory supercomplex of Tetrahymena thermophila 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-15900: Subtomogram average of the respiratory supercomplex from Tetrahymena thermophila mitochondria 手法: EM (サブトモグラム平均) / 解像度: 28.0 Å
化合物	ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-UDP: URIDINE-5'-DIPHOSPHATE / UDP / UDPYM / ウリジン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-LPP: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / ジパルミトイルホスファチジン酸 / リン脂質YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-FAD: FLAVIN-ADENINE DINUCLEOTIDE / FAD / FADYM / フラビンアデニンジヌクレオチド ChemComp-F3S: FE3-S4 CLUSTER / 鉄・硫黄クラスター ChemComp-HEC: HEME C / Heme C ChemComp-UQ8: Ubiquinone-8 / ユビキノン8 ChemComp-HEA: HEME-A / Heme A ChemComp-CU: COPPER (II) ION / 銅 ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質*YM / Discrete optimized protein energy
由来	tetrahymena thermophila (真核生物) tetrahymena thermophila sb210 (真核生物)
キーワード	ELECTRON TRANSPORT (電子伝達系) / Ciliate (繊毛虫) / mitochondrial (ミトコンドリア) / complex-I / supercomplex / Complex-II / cytochrome bc1 (ユビキノール-シトクロムcレダクターゼ)