EMDB-15867: Cryo-EM structure of cytochrome c oxidase dimer (complex IV) from...

Basic information

Entry

Database: EMDB / ID: EMD-15867

• Title: Cryo-EM structure of cytochrome c oxidase dimer (complex IV) from respiratory supercomplex of Tetrahymena thermophila

Sample

• Complex: Cytochrome c oxidase dimer (complex-IV) from respiratory supercomplex of Tetrahymena thermophila
  • Protein or peptide: x 53 types
• Ligand: x 15 types

Function / homology

Function:

dicarboxylic acid transmembrane transporter activity / mitochondrial respiratory chain complex IV / cytochrome-c oxidase / oxidative phosphorylation / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / respirasome / 2 iron, 2 sulfur cluster binding / protein transport / mitochondrial inner membrane / copper ion binding / DNA-binding transcription factor activity / intracellular membrane-bounded organelle / heme binding / mitochondrion / membrane / metal ion binding / nucleus / cytoplasm

Domain/homology:

Surfeit locus 1/Shy1 / Surfeit locus 1/4 / TraB/PryY-like / SURF1 family / SURF1 family profile. / CTF transcription factor/nuclear factor 1, DNA-binding domain / CTF/NF-I DNA-binding domain profile. / Tim10-like / Tim10-like domain superfamily / Tim10/DDP family zinc finger / Mitochondrial substrate/solute carrier / Mitochondrial carrier domain superfamily / Mitochondrial carrier protein / Solute carrier (Solcar) repeat profile. / Iron-binding zinc finger, CDGSH type / MitoNEET, CDGSH iron-sulfur domain / CDGSH-type zinc finger. Function unknown. / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Cytochrome c oxidase subunit Vb, zinc binding region signature. / Cytochrome c oxidase, subunit Vb / Cytochrome c oxidase, subunit Vb superfamily / Cytochrome c oxidase subunit Vb / Cytochrome c oxidase subunit Vb, zinc binding domain profile. / Cytochrome c/quinol oxidase subunit II / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Cytochrome C oxidase subunit II, periplasmic domain / Cytochrome c oxidase subunit II-like C-terminal / Cytochrome oxidase subunit II copper A binding domain profile. / Cupredoxin / Tetratricopeptide-like helical domain superfamily

Component:

Oxoglutarate/malate translocator protein, putative / Transmembrane protein / SURF1-like protein / Uncharacterized protein / Transmembrane protein / Transmembrane protein, putative / SURF1-like protein / Transmembrane protein / Iron-binding zinc finger CDGSH type protein / Uncharacterized protein / Uncharacterized protein / Transmembrane protein / Transmembrane protein / Transmembrane protein, putative / Transmembrane protein / Uncharacterized protein / CTF/NF-I domain-containing protein / Transmembrane protein, putative / Protein phosphatase 2C, putative / Uncharacterized protein / Transmembrane protein, putative / Chromosome condensation regulator RCC1 repeat protein / Transmembrane protein, putative / Carrier protein / Uncharacterized protein / Tim10/DDP family zinc finger protein / Uncharacterized protein / Transmembrane protein, putative / Transmembrane protein, putative / TraB family protein / Transmembrane protein, putative / Uncharacterized protein / Uncharacterized protein / Cytochrome C oxidase subunit Vb protein / 2-oxoglutarate/malate carrier protein / Transmembrane protein, putative / Transmembrane protein, putative / Uncharacterized protein / Uncharacterized protein / Ymf70 / Cytochrome c oxidase subunit 1 / Ymf68 / Ymf67 / Cytochrome c oxidase polypeptide II / Ymf75 / Transmembrane protein, putative / Transmembrane protein / Mitochondrial import inner membrane translocase subunit / Uncharacterized protein / Transmembrane protein, putative / Tim10/DDP family zinc finger protein

• Biological species: Tetrahymena thermophila (eukaryote) / Tetrahymena thermophila SB210 (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 2.6 Å
• Authors: Muhleip A / Kock Flygaard R / Amunts A

Funding support

Sweden, European Union, 4 items

Organization	Grant number	Country
The Swedish Foundation for Strategic Research	FFL15:0325	Sweden
Cancerfonden	2017/1041	Sweden
European Research Council (ERC)	ERC-2018-StG-805230	European Union
Knut and Alice Wallenberg Foundation	2018.0080	Sweden

Citation

Journal: Nature / Year: 2023
Title: Structural basis of mitochondrial membrane bending by the I-II-III-IV supercomplex.
Authors: Alexander Mühleip / Rasmus Kock Flygaard / Rozbeh Baradaran / Outi Haapanen / Thomas Gruhl / Victor Tobiasson / Amandine Maréchal / Vivek Sharma / Alexey Amunts /
Abstract: Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III-IV supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.

#1: Journal: Biorxiv / Year: 2022
Title: Structural basis of mitochondrial membrane bending by I-II-III2-IV2 supercomplex
Authors: Muhleip A / Flygaard RK / Haapanen O / Baradaran R / Gruhl T / Tobiasson V / Marechal A / Sharma V / Amunts A

History

Deposition	Sep 27, 2022	-
Header (metadata) release	Mar 29, 2023	-
Map release	Mar 29, 2023	-
Update	Apr 12, 2023	-
Current status	Apr 12, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_15867.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.2519 Å

Density

Contour Level	By AUTHOR: 0.85
Minimum - Maximum	-3.7738361 - 7.0050597
Average (Standard dev.)	0.004270913 (±0.5386689)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 600.912 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_15867_msk_1.map

Half map: #2

• File: emd_15867_half_map_1.map

Half map: #1

• File: emd_15867_half_map_2.map

Sample components

Entire : Cytochrome c oxidase dimer (complex-IV) from respiratory supercom...

• Entire: Name: Cytochrome c oxidase dimer (complex-IV) from respiratory supercomplex of Tetrahymena thermophila

Components

• Complex: Cytochrome c oxidase dimer (complex-IV) from respiratory supercomplex of Tetrahymena thermophila
  • Protein or peptide: Cytochrome c oxidase subunit 1
  • Protein or peptide: Cytochrome c oxidase subunit 2
  • Protein or peptide: Ymf68
  • Protein or peptide: Cytochrome C oxidase subunit Vb protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Structural proteinProtein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: CTF/NF-I domain-containing protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Ymf67
  • Protein or peptide: Protein phosphatase 2C, putative
  • Protein or peptide: SURF1-like protein
  • Protein or peptide: TraB family protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Oxoglutarate/malate translocator protein, putative
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial
  • Protein or peptide: Carrier protein
  • Protein or peptide: 2-oxoglutarate/malate carrier protein
  • Protein or peptide: SURF1-like protein
  • Protein or peptide: COXTT9
  • Protein or peptide: COXTT10
  • Protein or peptide: 39S ribosomal protein L9, mitochondrialRibosome
  • Protein or peptide: COXTT12,Transmembrane protein,Transmembrane protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: COXTT27
  • Protein or peptide: Ymf75
  • Protein or peptide: Mobilization protein
  • Protein or peptide: Iron-binding zinc finger CDGSH type protein
  • Protein or peptide: COXTT28
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Transmembrane protein
  • Protein or peptide: Decapping nuclease
  • Protein or peptide: Complex III subunit VIICoenzyme Q – cytochrome c reductase
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: COXTT22
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Phage proteinBacteriophage
  • Protein or peptide: Transmembrane protein, putativeTransmembrane protein
  • Protein or peptide: Lysozyme
  • Protein or peptide: Ymf70
  • Protein or peptide: Zf-Tim10_DDP domain-containing protein
  • Protein or peptide: ABC transporterATP-binding cassette transporter
  • Protein or peptide: YflT domain-containing protein
  • Protein or peptide: Cullin domain-containing protein
  • Protein or peptide: Zf-Tim10_DDP domain-containing protein
  • Protein or peptide: Annexin
  • Protein or peptide: Transposase
  • Protein or peptide: Tim10/DDP family zinc finger protein
  • Protein or peptide: COXBP,Chromosome condensation regulator RCC1 repeat protein,Chromosome condensation regulator RCC1 repeat protein
• Ligand: HEME-A
• Ligand: COPPER (II) ION
• Ligand: MAGNESIUM ION
• Ligand: CARDIOLIPIN
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• Ligand: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE
• Ligand: CALCIUM IONCalcium
• Ligand: DINUCLEAR COPPER ION
• Ligand: ZINC ION
• Ligand: POTASSIUM IONPotassium
• Ligand: Ubiquinone-8Coenzyme Q10
• Ligand: PROTOPORPHYRIN IX CONTAINING FE
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: ADENOSINE-5'-TRIPHOSPHATE

Supramolecule #1: Cytochrome c oxidase dimer (complex-IV) from respiratory supercom...

• Supramolecule: Name: Cytochrome c oxidase dimer (complex-IV) from respiratory supercomplex of Tetrahymena thermophila; type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#53
• Source (natural): Organism: Tetrahymena thermophila (eukaryote)
• Molecular weight: Theoretical: 3.2 MDa

Macromolecule #1: Cytochrome c oxidase subunit 1

• Macromolecule: Name: Cytochrome c oxidase subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 80.578977 KDa

Sequence

String:

MWVDFIDQTK SLKVSVNNYF YYLDRIKKLF TYLNDLRKHI LKKYVYTINH KRIAINYLYF SMVTGLSGAA LATMIRLELA HPGSPFFKG DSLRYLQVVT AHGLIMVFFV VVPILFGGFA NFLIPYHVGS KDVAYPRLNS IGFWIQPCGY ILLAKIGFLR P QFWRYYDK TSFSFPFLEK MKYNQYKEYK NDYLFYLDFL KKEITDDHSF FWKARKVIKL PQYSVFSFVP LKLMMWKTMI NY PESFWYA ASRVVQSRRK KVFVTKCSAR TLTTAGWTFI TPFSSNIKYT AVGSQDILIL SVVFAGISTT ISFTNLLITR RTL AMPGLR HRRVLMPFVT ISIFLTLRML ATITPVLGAA VIMMAFDRHW QTTFFEYAYG GDPILSQHLF WFFGHPEVYV LIIP TFGFI NMIVPHNNTR RVASKHHMIW AIYVMAYMGY LVWGHHMYLV GLDHRSRTMY STITIMISMP ATIKVVNWTL SLVNG ALKI DLPFLFSMSF LLLFLVAGFT GMWLSHVSLN VSMHDTFYVV AHFHIMLSGA AMTGIFSGIY YYFNALFGVK YSRMFG YMH LIYYSGGQWV AFVPLFYLGF SGMPRRIHDY PVVFMGWHSM STTGHFITLV GIIFFFLMMF DSHIERRAST STTLGLP RW YKRISYYIFK IRYLQHTKSK MNGIPGSTVR LMLINRHFVE YEVYEK

Macromolecule #2: Cytochrome c oxidase subunit 2

• Macromolecule: Name: Cytochrome c oxidase subunit 2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 72.388883 KDa

Sequence

String:

MWGNLWTEAS YQLNFNIGFS SLRSDVLIHL AQWQYWWWFW FALIWSFYYF IILKVARFRV LKMRPKISTS YRPHGKWGDF LACIIPLIW CINILTNSNL ILRLIEWQNE SSLFTVRVRA RQWYWIYKFE LKNFTDILST PKNIGNNRWQ INTFGELQTA D DYLHVLQL RSQNKWVKNY WNRSLQETGK TNKAHVISPQ EQLRLSLINQ YKSLNLSSSI KHNAPFINRD LYVFDDLFSY NL GDITTKK SLFNDKNSFL TSYSYLNNNS WNNNEFDLID NLPFTTLFDN NDLFNNYKSF FQDSIFNSPK KQLSSDSKQL FKH IIYRSI KNNIIQDYTK LVKHEDFDEY SRWIKRSPGE VLPLRIIKYP LGLETIHNNI FENTNNEGNV ELFRLRFNSN SSKM QHKLV QDTIYLTLKQ KRYNRKKVVA PQIKYYKDDN GNKTDLVKYT GKPYLSNDKL LKQSIYDQTT QYKLIKKNKK RGELI PVTL ARRILRTKKT LVLPAHVNIT LITNSYDIVH SWFIPGLGIK LDCVPGRSTH HTFFIDNVGF YYGQCAEICG RYHHHM PIR VCALPFEHFL LWWNTFGLPK MLNTVSRKRF ETHYELRKYS W

Macromolecule #3: Ymf68

• Macromolecule: Name: Ymf68 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 72.782539 KDa

Sequence

String:

MLICNFLMYS NFSRIYWFDF NGTVNENLPL NYNVLKICRN EINKLEKLNE NNLGTQKNPI KLNLSFEDKH YNTNNLVLDL NSYETFNSK NFISSIFDKT FESLNTVLMA PIYSFLEFKL KLSSTKINTN HYYVINGKLY ITYNDSFKLF TTINDYFNDL N ELSNTKLF FLYRSFNIYN IKLNSLVDFV FLKLILFIHL LYLKSTNYNR FDYRLKQTDW GFYINNNSNY IQNIFSGLKY IW RGLRFWI IGLLLGLSSI YYLMYVRLLP FNKIIFAWIL VAMFLYWLLS GFVFFVKKYQ YSKFTAAIQR FWKRTYIIFW VIE AGTFSV FFYLTLNASS EPVYMYDQIK IYKTHLFSWR WFLIKLLPSV SIILLGYYLQ LTLKWNLFNK QNTIVLLITL LLLY ILWLE FYQFYHILSF YGNINWAFDY DEYIWTLELD TRRTRLANNY IAICLFAKFW HFVFIFLFWV FFVLRINELG RIRYP LLVA NVQNFIIIYI MSWAYMYPWL KFIFRKYLDV PYYWFYLNGR ELGIRVFFTD LKLFFYGITN RLFDFNPSSI KFEKYP FYY WINSSQLTEF NQYRKFVIRD SIIYSLNNYI I

Macromolecule #4: Cytochrome C oxidase subunit Vb protein

• Macromolecule: Name: Cytochrome C oxidase subunit Vb protein / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 75.431312 KDa

Sequence

String:

MKKQKRTQGK QNTKQIKQEK LSSKRKANNQ KEGKKKVKQE DYKEIKQKGK RMLSKIVKAS FSSKGFNLAN AVNTVKSTLN APIKHIKRN IEPTGSNYSR MTNTTEEAFD EVSHEWQALV TSNPFDLNVF NYLENTQTSN FGTVDNPLVV FTSETPFRYV G CTGQMNED DYEGHELLFF LLREGSLQRC MGCGQVFKLV RLRNEYSPEM DYYLSNFHPY EMQEMGESDT TVLMSPYKYA SH YEYTQFE TPSNMVYSMV NPDEHDRLLV DPAYRMERTK ALEEKYKVYT SSLREVEKQF EERYGRAGQI NISKVTYSTL IDV EKAVLK MDRLFRKVAK FENRAFIDRA NHSRREKRML ERAQQRWDSN YSFFTGSLTE EEQKYRDYYE (TPO)ELEAYPEDE GIEQQLDQQ EVLLSGRYDP KLYDFQEGYT KNPEDDQTSL IEKKAFKFRY RLANETSETF QRRNNRMVER QIKRFQQPQY K HAFEQLQK NIAISSNSGN ALHSEYGYLE LLSNESVQLY KDYYE(SEP)DAEE DFKVFENLSS KEKLVMIANF ENNLLPKY D RSEVHLIPKR QWEPAFGVWE NFLYDITEYA SFIAPRGKEI AADYQIQSAI PLTKEELIEA GLYKETIEKK VEPKLEAKK QTKSE

Macromolecule #5: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 15.618258 KDa

Sequence

String:

MIWKYLQRTN RGNIIQAGLQ HRKFENLPFK QNFDNLTKAY DLRMWYISNS PHEAKNLEYV NELEALHNEL NYQNSRQFLF RTVSFLLGW ALFYQFYELP KTYDWQDTQE PKHQVPAYGD LEEGGDEGGD D

Macromolecule #6: Structural protein

• Macromolecule: Name: Structural protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 27.946445 KDa

Sequence

String:

MSSAVEKKDL PADYGKMPAG YNFLTRGKDW REYDKDFILR TDAVWEKFQL EHFFRNYMKC FFFDHGLKKY QMFEPEDMYT VVFEGWALD DLITFPGFTP TGRTNSYQIG LSPRQRTVVP TQTFYQMQDY YMLCGLRFER WFRCDLVYHD QRHTKFDQVK N QKNYKTYP CYREYYEAQY ACQDDMFDFL MELAYARRAA DNFESDFASH ELTTLPTFYD TPKAAERKTY TY

Macromolecule #7: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 12.786321 KDa

Sequence

String:

MGSVWFRNRY WWYRSLYDDY VAREAKLAFG IAAFIWLPHY YWGIHLNRAF EVNFSHRNYA HEWGPRRNRL AHSLEFEQFD MILENWQDL EDEYAQRGDG MLKK

Macromolecule #8: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 16.427672 KDa

Sequence

String:

(AME)NRFFKVSSK YQYYKYLEQY DAAFLRKYQS ETHWYLGRRG AWKNLVIKYA GDHISLEEEH NVKYKTHLSF VYLSYR LAW VLFAYVLIYN HFLLGDIGKT FNVGEWDHRL KPSAERDYPT RYESLYILDR TQKW

Macromolecule #9: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 28.666662 KDa

Sequence

String:

MISKYRYLHC ARKLVKQSVQ AFGGGHHHHE YDWRDDPKVN KDIEEDIRDR GWHPETYDFP YTKKHDDWVF DVTMPSQNYQ TDLTVNIHP ENKKMHVMKQ VMRQSYWDAE HDMAHEYDYE (SEP)EDLDFQCES FKSQHFRKKG PISQYLILGL LPILYFG TE FFYNHYPDED YWRVAHPPPL DYPDTDDTDD TETFKDYKSF TGRRMVDTGI VDPLWYDIRE GKKVYYDWAG VNQPMEDI

Macromolecule #10: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 26.601174 KDa

Sequence

String:

MSLSLFGVKN NWHKNGIWWF SKILNKTVGE ERYDALRVQR RIWSMRFYYA RQQCLYELFV DHPDLAQWTG TYPKVDSSHG FPFYSTYEM YRDFQENTLN SDGSFAQWIT LVCGIYVIHV IYNYMIPYYW VSTPLKNDEF TRLRMKDYIA STVLEEVYGI S YAEWGWLP HDFAYNRMRG LAGYMHPDDP RAMCTSTFHR KHKYIEHEVE KVGDYHHMTY PK

Macromolecule #11: CTF/NF-I domain-containing protein

• Macromolecule: Name: CTF/NF-I domain-containing protein / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 116.81275 KDa

Sequence

String:

MNKSQDEVKE DYFSIKKKKY NSLDCVLSPI VTRKKQESIN SFQAGLQEDQ NSTAVPESPF IRNCSETLNR SQHKILDHYF EYDYSLKKK IEKIKISLQN KTGTDSSQSL VQKRLDDLRK KTLIYKQFNI QKNNNNINNT GQFFIEKDKQ NNLNLSQPHQ I IKSKQGHK RSFTNTLYCS SNDKKIFNIS SQKQNIKTVK QSNNSSMYEE QIKNDQNKKQ NFNKQAKSER KQAEFCDLFY ES MQKDAER QFKSNSDKSK SITQILENNC ENAIVLLNHL IQILTDSIPI SKNSILLDEK LSQCVNLSNN SKISQLMLVI QKL QGKISY YYNDFLKCIR MMKICKNFCQ LFGTLKFKIS CYKYIGLGFQ RLNKPKVALV YFVKMLRQSW FLKQEDQEIN SYDF IGMSY FYLGSIDKAY QYHQMMIDGI KADEEVKKIS NIMLKNRKYS NYPTLQNIRI IQETSDGQPL QKTIRKSKII NPQFQ LTQQ EWGENISSSE DEFELPQMQI AVKNAEEQTF QKKFLSTPWN DRFIQAQIHK LEIAQELNYN KFPIGLVFKN QSVLNF PNS SNQPQLVVGP YANKIYNQEL SSTYSSRQRI SHQTPNREFF NYHSQLQQVK EQTQLNQMKQ QYNQIVDSIE LANHLIV LK NLKKVKDLFQ NLIPYLNEKY YNIKQQNTKS LKQQQNSFDA QNSNQSFLGL QNYSIQINGI LNINNQTDEY VNNQSDRS Y QQQSDKLKLL IKSKSRNHSK EINMTQNYFK SPSNESKQIK NLRVIVDNSN KKKAQQKLNQ EFSMNFCESP KYDNSILSL FINKLSQESA LRLEIRQIGS ASRYQRKKQQ KGKQRKEKYQ KKQEENKLNI KQYKKERYNM ALFFMANTAI SVPSVLDTSP CMKQMLAYE DCVLDANVPQ QEVIHPQWPT IWPRLLTDGK LLEFDKVPFH PENIYNYTYM RPLTKKNKQY LYECEEERFV F KACLRKVI SLKKTDKHTS WDTAEVANLQ LT

Macromolecule #12: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 57.814465 KDa

Sequence

String:

MLSKVTRRFL NYNQIYCFAS QHGAEHHKLT ASDEAYLNEV RQRYVTPDME KWAYLDYKKH PSTTLSHYDH KSKDYVESER DDYNADVAT NSHNKLIDDF KRNLQMQRKV HDILQKMDRP YLRGVPGVTK NISAGLQDYS APVSKKSQSD PNDFYRDAYR N ENRWIDQS VFTPKTSKMT HYDVEWPKEL ASRPVTKKFH HDKGYKYDVT TPYDQRYNYV ADRLGHPEIL GNPFERLMRL EG DIYHPNY LDQPFVKVPN ANPNASLNFE EGEVLYENTR LLEWAKFWNY SVVVGYLWCA YFVPYNIFFK THMPLEHAYD NLF FPYFQH THFLWDNNAL HIPTVGGVAI YATYIALSYI NNIWKDYVVR AQFSKDKELL FVTRVSPFGT TEEEVYEVAH LEHL PPSVR SGVKDLSAQD ADGLVDVTCM SSQRSLVFYK GDQYWNPKVY NDFINQTSNL WTRNYTGYNR LEVQNSVEQV KIGFS HSSQ PKLEKK

Macromolecule #13: Ymf67

• Macromolecule: Name: Ymf67 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 54.538531 KDa

Sequence

String:

MTALFLHILW SISYIIINIL YIFLSLLLSN NNEKIKQYNS NYFIKILLVL FYNKNLSFYK NLLSEDEISK IEFERLKNYP TLVLIHSNL NKLEKRNKII NSFINFKTKY RFYKFISTNF NLQTIIKNCN DKIIFSTLLY IVNLNYSFFY KTIKNTDLIV Y LLANKFSI LNDNIIVSKF NISKFNDYIK YINNTNSIDT YLENQIILGL NNNTNSNITK NINTKLLNSY SNLKNLVNIT NN TFYLKKI NDNYNTVINS EFLTYLKSNY KISFSASNIV KYLSDKSVNN SVILYLRKNK IFNKSRYSRN RQTYRTGAYW CLY VNIIAV VAFYFWFYKF TMNFGYLWWL LYSLILSFFF SRALKHRFYN PLNVMTEFKN GFMWFIIILI NIFKPLLKLL ENNY INLYN HLVIKYYQSF ICNTLINKKK LEFNYILSSF KFIKELNNII IISLNKLF

Macromolecule #14: Protein phosphatase 2C, putative

• Macromolecule: Name: Protein phosphatase 2C, putative / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 53.011297 KDa

Sequence

String:

MFRRIISNGA LLSTQTQRWQ DLSKFACLRA SLNKESEKAF QELAKKNNVS PQELVELSKI VSMNLDVLKQ NINSEQFLLE KESTLKRYR QSSIGTRGHL QTVNEAVNTK YPTLAEGLGQ VAGYKEAYQA LREIFVHPSI SVNNLRQGSY GQQFAVDFRT R ADEYVKAL LKDHSSNPQA VQTIQEIQHT LHQIIKNYEQ NPASIYARIL TVLQTRGVNT LPVSKTADQK AVATIQKTST PS LTIDQLT VPVQERVQTQ TVFDAELAFI KEANEMIQQN TGNLPWDGGK KKIFQGQANK YLETPYYLLA ALSGLGLLYF LYS GDAKYK TLVLTPVVGI AAFVLLRRNQ ILNRVPTLTE LFLHKDGKFV DAVVSVNGQL ISKNDIPVST LKLYRGDHTV KVNL NDFED ASAKKFLAQQ SGQEGVINVH FSKLRNLAAR NGQVLNLGDT EVVVPFENQA NRIILKQIFK GVEVLPSS

Macromolecule #15: SURF1-like protein

• Macromolecule: Name: SURF1-like protein / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 47.087973 KDa

Sequence

String:

MYEGRWKMVN QIKQDVQNDI ELILRQMNER IHAMKKKKYN ELKGKIKQKC IDLLIDYAKQ KIGKKNKIKK EGNNIRHNKQ KKLKNIQKK IFKIISTRML QTTLSAFRPR GSNVTGKVAL ATLGALTGYG AFYHYNQYLN LSARWQQIQE NIAKDQPFDV D GFDAKVYP WVRENNVNDW EYKLVKMRGY FKDQRFFVRR KRDGKEGFLV FAPFVTAVER VNHRLKQKDL LPVEYSVFVN LG WVPVENK KDVELGGEVC PPMDAPTDST LFVNDTFTGF NPDPANPEDT EQVTLTEITG IVRRGEQQDI LARRRNWNKE GIY NWVDLD YMGKIFRLFN LDAINTAYIE RVVPSFEEGE EGLYPIPATK DTFERPLNTP ERHSTFFNFY AATSALSFIS MLLL RR

Macromolecule #16: TraB family protein

• Macromolecule: Name: TraB family protein / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 45.064949 KDa

Sequence

String:

(AME)ISARAVHRF LRNPNLETGA AFRAGTRFDP FKNTLTVLKD PQNGRTLYLI GTTNSSTLLA NRTKDLVQKE KPDAVF VQT NKEWWNLAKN IQDVKCQQEL NRYNDLLSQA YTLSLDNTIR NLVFKAKFYS WLFVINWFKA FPDDFHPFIP GLEMKFA IE EANKQNIPVV LGGLEVDDVT LSALKVEPRL DPFSQLYYGY RALHNSFWRR EHFDNYATLD VVGGEAYAES MDRFRTNW F VKYFEKLAPY QKKIIVDQKD LDLFYALYRD TPGKKIVAVV NQWHVPGIEN HWKSATNTHE PLKAINPIGD MDINKYMES QLVNDTLRAF VSKVGKTEPA TWKNYSTIYH KDNYEAERVR HVAFVDHKDP HMYHGLPQDY DDNIKPKHH

Macromolecule #17: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 41.307387 KDa

Sequence

String:

MRYLKIEKEK LVSCKKQEQE VQRIRRRKGN QKLNSIAKQQ RVKRRDYQQN IKQNKEVKNP KKLIKQQIIN KVKKRKKMFR GLTKFNKVF ALNSFKNSLV AVPKANLNHV QNMLEENLKY DAQKYNDEVA VIQKTSRIYK PTYTIEFNRE GEVLVYSADP I KNSVVYFK YPYVLYEAAI PLFIWAWIYN PLELSKNAVN SLLIYPNIAW IPRMWYWRSL QYKIQKMYLL RGGKVAKIET QS LAGDRFT SWVETYQFHP LTQDQKNFDN QDNAEFLEDE GQLKYELGVQ LDNLQEMGTT SQDIVINFMK EGTVHHPELF EAI VKGYNI DTSDYVINTA NNLRAREGNH NH

Macromolecule #18: Oxoglutarate/malate translocator protein, putative

• Macromolecule: Name: Oxoglutarate/malate translocator protein, putative / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 40.43427 KDa

Sequence

String:

(AME)FFELEDIKR RHSLYWDIYN VQGWVRRPDS TLYNNVKRGV TAGVVASLVQ ENITALVENC KLLATKYEKP QNLRQA ATF MKEVFKLENY RKAVWNRSQY ALCIGTFDIG ARLATFRWLN NGWQRVFAGF EFNFVRKIPT TMLAALFTAP FSVPFEL AR MAYYGDKTFP KELQRGYSSY LSALARIPFE EGPYFLFKNS FPLIIRNFFQ TFTLFYTYDF LKDKASFAWR VGEQNEYA C KMIIAGISTY LAAVFSYPWM VTREMVDFWP KVPGAPCTFN GNYRKAAVWI WYHEFSGNYF AGFFTKYFWK ASPGMFLTL MLADKVGLFD QTTVDNFGGA GNNSWEDTFV

Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 37.55298 KDa

Sequence

String:

MFLNRLVKET SKAKRLFSMA QNNFARAGPY NPNRYKDYYI PRTLPKNEEI VEFVQSQHSV PASPIRNQRH INPVRESGPL PSYDGTYTM EDIRAVFYNT TVGRDYCYCQ MDPEEIMRRV PGITRKEAEF ITKLGLSPQE QVDFAYIAYN IGLDIFYFTN Q MFVARQVV TNSKGEKVEV LWNAQCYEDI AQLNVGFAPV LESVDYHWEI FLWADPPIKP NNDFDLNVPC TWFEYEQEWW ME SCIQEDQ FNLPEDERPY NTPRNPHCRK ELWRSQDALQ EEELMVNENW YPKNTQYNIY NQPDFIKPKS GSGAAADDIR I

Macromolecule #20: Carrier protein

• Macromolecule: Name: Carrier protein / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 37.095141 KDa

Sequence

String:

MANFVIPYLK PVADFWNSLC IDQHQDSLFQ FKGQTGSLGT DWTSKYLRSE QDVYNHKYLQ YHKRVHEAPE LTDVISDNVY RLTLFAGVE RVLSVRQAQA ILKTQFAGAT ENISGAFQTV LNGGIFRRGY FRGALLNLLQ FCGAPYQSLI WSRNSGITNQ V IVSSIFEA FFYPLDTVKT LIYNDVQGKY KGAFHCASQV VQNAGWSRLY AGIFQKLIFN SALIFHLNQV WDGSSQQWAS LA LVAAAYP LLVLKTRFQV AGTPLALATS NEVLKVNRKT LYAGLVPYLI FNTLFAYEFA AWHSSTAQER VIGGLQNAMK QFS SPAAEQ VWSS

Macromolecule #21: 2-oxoglutarate/malate carrier protein

• Macromolecule: Name: 2-oxoglutarate/malate carrier protein / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 36.132316 KDa

Sequence

String:

(AME)NYSYKRYWE PSTAEVIGLS LSVNTISAAL TYPIEFVKVR SQIRTEGVGI RSKNLYMGIN PNKVFREIHA TGNGLR GFY QGFESHLIGR LSYLFIRNLT YKIIYDRTKP VKAHNDLSHR EKGVIAGFAG GLAAFLTSPA DLVNTRTIAE GGKPKEW RW GYKGLMDGIN KIAATEGGNA ALFRGSYANV LRAVILNISL TGPFDYLNEK IWITFGDMTW NKYAALLWAS FWGSVATL P FDNIRTRLYA QNADPTKNRL TYSGWADAAK KLIQHEGISG FYVGFYAFYI RTFLYAWTTV FITDKITSDW KRKAGLKEW QI

Macromolecule #22: SURF1-like protein

• Macromolecule: Name: SURF1-like protein / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 35.794367 KDa

Sequence

String:

MLQQVFGKSK GGEVTNSHLT PKVLNKAQEA EKLASQIQAQ RFSNRLVAFS SQYPRAKLFF AGIGIGTLLY GANQSSKARE NKVATETRK ERMAKPTIQL TGADSQNPPF TEKNINDWLY KTVSITGRPI HGKGMMIPAK SYGLHGFEYL VPFVTKENED G SVQEGLIL NLGFIPREYA PIWARARVEN VEEQTFTCVV TDGKHLSEQG GLFASNKPCE NQWEYADLDQ LAKHTGFVNQ EQ VRSCILE HVNTETPNDE RDCRHIDICS DYKEDYPYKF TRSGVLQQPG QMYWDLNKSA SYYSLLGLGC SVFSALLFLA K

Macromolecule #23: COXTT9

• Macromolecule: Name: COXTT9 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 29.865686 KDa

Sequence

String:

MVYHLFERIC NPDNFKLSGE AARVRTLIAA GFSKEEAEQV AWLQNHQVNG KILGLFTGGF ALYCCNNYFH YFERYFPRLR YQPFTKFLA QAATVYFFFK IGDYYFTSRR YGSNDARMNG LMYSNTYYST NKEALIQNFE PLNRKFTEEE VEQFLRNEGR S QEEKRNWI YNPHIHGSTE GEWKADIHEK FDSGKAPWER EHVKAKILET NKAKIDAGEE IQLKPFKTLN HLDKTGLLHR LH PFIWTNN WTLLG

Macromolecule #24: COXTT10

• Macromolecule: Name: COXTT10 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 28.101486 KDa

Sequence

String:

MSSFIQYEFL KIYQGNQKIK NYYKRKRLIF QQKKVLKKKQ KEIQMSTNNL RLKPWFHWTD EERSHAIFSA YEKRILKSED LPSFLRANR INNVSTWVFP LIALPLFNQS IFKLGFAQRI LLTRPAIEWH CFKIATVAAS WLAWLNFSPF YRKLENEKEY L LDTLESRI GINVLDLNDA LPRWTTSQEY NRRTQQLYNQ RNGFFAGLLY PQEESSRPLV DIASFPKNLH KEKLTK

Macromolecule #25: 39S ribosomal protein L9, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L9, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 27.034711 KDa

Sequence

String:

MFGRLVLKQT RRTLFNPVLK NTFCIYQAYQ NPLRHINTGH NPNNVYEDIV MLGDYPVQNR THDKVISQTY VPAIANIAFT HLSKKYPQA GLKVDQLNTL KEKTWNDLGV NIEHEKQEIL VELSEQIFVK ESKLRWVHEQ RQRLAHTTYV FSGLEFQNVK V GFFIDSYN FLLQELAHRS NLYQSKDIVG EKSFHEKHLE QQTAPYSGVK SLEEPVSQNK SFINSLMRAI HNH

Macromolecule #26: COXTT12,Transmembrane protein,Transmembrane protein

• Macromolecule: Name: COXTT12,Transmembrane protein,Transmembrane protein / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 25.953754 KDa

Sequence

String:

MYVLFVCLID SMNVEEGKQI KEMILPHNNR QLARQYFDSL PENDINRKYY EGLKYETPKT FFGRFLNQFN IDAKLDTLSK FYTYQKTIR ATQAELQEDR KSYLTNSLLF TAVSWFSIYQ FARKGAVLPV LREYGRYFGT HRLFRQYLHT LVLPLLYTEY A LNQKYYTH MEHLWTVHVN RLNQKILEDP LYTFYPQELN VPKHNIIVPT IFRDTPQ

Macromolecule #27: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 24.701131 KDa

Sequence

String:

MKKGTASEEE LKKLYDPNTF YEHGDNPAFK QFMNIAVENL REGKLTDHRT YVVDTYKKWM YARNWDDFLQ RDCKAITFPR AFALWIVGT LGMATASKWC RQILPVGSHG ITKISQTQFF HQFGPLGTLG AVGFYGLTAY LYYKTTIFTV KKFYSHCILQ E REWIFEQE RQNPGYGEYF FKDVPLSAEE HFNDLARGEM AKKKFEKPNH EF

Macromolecule #28: COXTT27

• Macromolecule: Name: COXTT27 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 23.368416 KDa

Sequence

String:

MSALLKEILA LTVKSEAALW KGAEQKVLSG LNNLAKTELV QITHHFGVNK QGSEALWSQL DKAAVGAFPE LSVDETLQLI DGFGECPDS YTLSHDLNQR LLVSWEQLGK LNFQKLKETN PYFASDIVNQ LDAAAAEFIK VRPAAESEAG GFLNSLGVSS S FNTTKNDI YVVQSASGKK LNNKEQREAY VLEKAQKYLK EDPQSKILDI IAQK

Macromolecule #29: Ymf75

• Macromolecule: Name: Ymf75 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 23.325801 KDa

Sequence

String:

MFLGIFKDVI KLLNKKVVPV YFWFFLYCFL STMDTNIFVS SCSFLKVEVF GKDENTTLVL LFYVFYSLFN FYLSRIKNKN NYLVRKHLY TTELLIELIL FKYKLIILKF SSIKYILNFN VRKFILFNLF LINNYKAYKI NTFFLYIYIY LNNLNIIWYP I FKAYSIFG YYKSTRLNFI DTKNENIKRI KY

Macromolecule #30: Mobilization protein

• Macromolecule: Name: Mobilization protein / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 23.25766 KDa

Sequence

String:

MKEKIFNELT RKMKRKEISA KIQREENKQI LIRQRNNKKY IQSIQGIQQE RKKGKLYLVE MATQNVEEMD TIQKMNYEAT VNMGRQDLI TREYTFYSDY EFIPIQEDRK QQMEDALNNL HKIIHPTVTQ LKKKANVQEI QDRVFRKLQG WEGELNTCVF S AKNVRDSN FCADRFTNRI NTEGVEFVKQ ILREY

Macromolecule #31: Iron-binding zinc finger CDGSH type protein

• Macromolecule: Name: Iron-binding zinc finger CDGSH type protein / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 21.476887 KDa

Sequence

String:

MSAILKRAAK YKRVSSILCE GEAHLRDPFT PPPVILKPPA PRKDKKPDDI TDFPAQKLIP LPESIPYQEG KYRPASIPMV AGFFPYNCY LQQGKVYSWC SCGISQSGPW CDGLCNSVVT RCRPVVFNVS QSGYYKICNC KFSANAPFCN NTHRKMVRYH H QTHRGFYE IWGAALFVLG WVYMGFNYYT

Macromolecule #32: COXTT28

• Macromolecule: Name: COXTT28 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 11.121555 KDa

Sequence

String:

MAARDFEYNN QDVNQLNGAF ISLVEDEKIG FWVGVGGFAY SQFIMRKFVK STNIFASVTS LFAGAALANL YTHQSRASYA RVAARANRN ASLALNKLME Y

Macromolecule #33: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 20.388264 KDa

Sequence

String:

(AME)DNNYHFWGN GDRQDVSLSY EDYYSILDCL LDEKLSPQGL MKFKNLHEVS MYGVSYVPLY CFPVAYGISH MLTGKV RRG HSGYRNLFSL MSVVLPFTCW YAYTTPIPRR LYTEIICSNN ADGAYVRNRI KQQKPGIWRK LSQQLYNKNF RFPELNQ DL TATEFPLDYV APHKF

Macromolecule #34: Transmembrane protein

• Macromolecule: Name: Transmembrane protein / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 20.025109 KDa

Sequence

String:

MVFEFLFYNQ QHKTRNGYFI NHDNLMLASL EERKKLIFYF IANQVPEKLD PVDRVKFNEE LSDNLSTKAR LIGSLTGLIG LVGFPYIST RIYSRPVLNI GLSLLICPFL YYVGNQLTYS VWEPKFIANN NTVCELSKKY NFTVFDFAQA KKEAHLKALR T ELVSDNLL YSPGI

Macromolecule #35: Decapping nuclease

• Macromolecule: Name: Decapping nuclease / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 10.242719 KDa

Sequence

String:

(AME)EVKYRGPSD DKLECEFLEN NLLSCLREKS VQDNVAKMTC RPEFLVWFFL ECPTKAAVYH DPKGLRNIFI QDKIKQ KGS DDGVLSKDD

Macromolecule #36: Complex III subunit VII

• Macromolecule: Name: Complex III subunit VII / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 19.882758 KDa

Sequence

String:

MAIRNFVFKI SNQIQNLAAK RSLAYLNQID SQSVPSRATI NMKDQVTQMQ REIDNMANVI RAQIPDEDRA EFEILKKYYV TGQHDSLVD PQDVLLQLDR IQVLKNLKMI ELNEEAYDPE LVRLEKLKAR VLLEEEGALL EYAHFISKRP YNKPYEKWGV S EEHVKQQI LG

Macromolecule #37: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 18.801604 KDa

Sequence

String:

MGFETVVPAP PTRDDELRMI KATEEQFLQQ PRYKLYMNEA HRIAKMNHGD RHNNIRAHFW SNFALGLLIT GPIFIIPFGK AFRNLRSGV PYYFRPKYVF TQKNQYNQDR NWGAMKKQIP LWLGLSTAYA YWFTDFSIND DEWLEKGKVI YPHQTIKVL

Macromolecule #38: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 18.600445 KDa

Sequence

String:

MSCTTRRFID EKEKLEYSRG YNQQELEASK LRKDFVKKYI VDFDTTLYKT QVERDWAYIA KREYRYEVQL KSIGYGGALA NAVLLWRIY ANKKMVFWPI PIVGALGYLY FQPVFFQKSN KRFFDMCNVG EEYYLGRERN KILRECNKIL NVEDF

Macromolecule #39: COXTT22

• Macromolecule: Name: COXTT22 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 17.701115 KDa

Sequence

String:

MGKDQLDFSH FDKAFENKYD IVAPEFGDLH QKRAEFIAKN QGTYRPVPLV PNNIKGLIPK TCRLPATRNW YRRTSSFERN GFFNIHTPV LNTKMIPWLL FIVLTWGWSS FQIGGYNYER FDDNGERRNT LYWKLSPVEF PQSKLWNRPS

Macromolecule #40: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 15.588598 KDa

Sequence

String:

MVFHYTNFVQ ETNAWWLRRV RPVYCTVLAY YGWWLYDRYY LFGKNATQDI RKDTTEVWEK RAALNKRNWG YNAHYKPELE RSMKKVLYA DPNYKFPIEW PERYMAETKT LEQVMDEEEN WEYYK

Macromolecule #41: Phage protein

• Macromolecule: Name: Phage protein / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 14.740897 KDa

Sequence

String:

MMQNLKKFMS KTIQVQPVSF NQIPKAFYNF PEYRTGGVQA NPGITAKRII KCIGERLRKY DPARWENVPI TFKTHFRDEN GYSDVATSI QIHDALEREF GIDIKDRLAL VTDVETAFYI VMSHHDPL

Macromolecule #42: Transmembrane protein, putative

• Macromolecule: Name: Transmembrane protein, putative / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 14.344524 KDa

Sequence

String:

(AME)EPFGTDERN WTHEEKDIIT RFLKYDKHVN LKTAEMVYSA EVESAYFGKA GALAGGVISA LFFNFPIVRN LPIIRR SVI GVLPFLYCYT WGKNTQEELR WLKTFAAYQR FVVYHGQHCK LWV

Macromolecule #43: Lysozyme

• Macromolecule: Name: Lysozyme / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 12.273913 KDa

Sequence

String:

MAQTAHQNRY QGGLCYAQCN ELFSFWNPSI QQCWKGCDFG VGRVNDPEGR IEAQQMCKRW AAELYWTYKG ELDTIKDLRV HADMYPTTP QNVYRACLAG VRRQKF

Macromolecule #44: Ymf70

• Macromolecule: Name: Ymf70 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 10.89192 KDa

Sequence

String:

MFRWLFLYWY NSTDTPSAIA KVNLWSYINL RLFKARLSSS IAYYILGLNN LELKKLKIFY KNTYFDYIYL KSIPCLFLII FFTNLYLFL

Macromolecule #45: Zf-Tim10_DDP domain-containing protein

• Macromolecule: Name: Zf-Tim10_DDP domain-containing protein / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 10.435701 KDa

Sequence

String:

MSTRKIFDSE EQSFIRLVDK FYLGLSLTKL CAQSCNLLRN DISGSALTQK EKDCLSICYN NIEKTQSAFY AKVKTTMNLP AVEDDGEEG GDDE

Macromolecule #46: ABC transporter

• Macromolecule: Name: ABC transporter / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 10.418697 KDa

Sequence

String:

MSSDPFKKVE RDYHNERSVH KHFASYPLKF WWGLNKFETI QGIHSILGNA ADLVVSTLSF IPGVQGRNNA SYIENSIRVT RFRGFDDKT Q

Macromolecule #47: YflT domain-containing protein

• Macromolecule: Name: YflT domain-containing protein / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 19.97932 KDa

Sequence

String:

MNNTFKFLHQ VISKLTLKAQ VPNYGQYSHS LKRPINPKVV VFGNSSRAYE LISSQFRNFN HVNGLELKGQ EDNIQANKVA QSVLSINDG FQDGYYITDF PQNSKQAERL DLITDGVNLA LYIKDPSDKV TVTRQQEAID YYRKTGALVE FEVDPRGDLE E QVKQLSNQ VLNGYKH

Macromolecule #48: Cullin domain-containing protein

• Macromolecule: Name: Cullin domain-containing protein / type: protein_or_peptide / ID: 48 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 8.965917 KDa

Sequence

String:

MEDNYAADVQ RQFNRTAFDS LYKICYNSLV QKNGSTIDFQ KQIDCHQRLI QVFAKIAPIV VKVEQDAASS GGAAAGGEDE E

Macromolecule #49: Zf-Tim10_DDP domain-containing protein

• Macromolecule: Name: Zf-Tim10_DDP domain-containing protein / type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 8.354574 KDa

Sequence

String:

MSDKRKIQHE GIIALINYST LCAQKCDVLK GHDDKITDTE EQCLRVCAEK IRQTFEFTND IYLKNPNLTK PN

Macromolecule #50: Annexin

• Macromolecule: Name: Annexin / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 8.15536 KDa

Sequence

String:

MDPVLGDVIA TRIYKACFKH VYGKNMKAYS EKDEAKFDQC LTSYVESYKS VTNHFITYLG QLPKKGLSLD GS

Macromolecule #51: Transposase

• Macromolecule: Name: Transposase / type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 8.081111 KDa

Sequence

String:

MEQNTTQVFS DLAYKVCFKV INDKNKPFVL HDEQRLANCL TRYVEAFNVT SEYFFRERAG ETKVTEKQ

Macromolecule #52: Tim10/DDP family zinc finger protein

• Macromolecule: Name: Tim10/DDP family zinc finger protein / type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 8.00899 KDa

Sequence

String:

MDTEVSNHNY TQAVAYLNRA TSSCVKKCDS LNNNGSLSSK QESCLKTCAE NHAIATKIHA EYIRKLAESK YL

Macromolecule #53: COXBP,Chromosome condensation regulator RCC1 repeat protein,Chrom...

• Macromolecule: Name: COXBP,Chromosome condensation regulator RCC1 repeat protein,Chromosome condensation regulator RCC1 repeat protein; type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila SB210 (eukaryote)
• Molecular weight: Theoretical: 58.69782 KDa

Sequence

String:

MYLFMHLCMC FCMISSSQKK EKLNKGKNKQ IKLNKKKEKQ NKKIEIIKKE KERRSNPQMI SKTQAFARLF QLSSKSFSTV KGGNLYTWG QYASGTGFET ASAVPRKVDY FSGNVSKVAM GPYHTAVITN DGSLYTFGWG QNGALGNGAK EFQLSPSGNL Y TWGQYASG TGFETASAVP RKVDYFSGNV SKVAMGPYHT AVITNDGSLY TFGWGQNGAL GNGAKEFQLS PSPVSFFNDK KL KVKDVVV GESYTIAVTE NGEVYSWGYG GEPSSKINLD FFRNAILPQR CGALGSGDNK NRLTPQQIAN LKADGYKNIS GGD NFATLV NQSGEVINWG TGLFGSLGNG SDYPLFTPEV NAYFKHLKEH EGLTVQSIKS AGHFSAALLS NGKLYTFGVN TQGQ LGIRE NLGHNTDQNA RLPTPVVDRH FVGQKVVDFE VGENTLVFLT DKNEVFFSGL ELAYQPIRWE IPTDKKIVKL AASKD TFAA VTETGKIYQF NEFVGVSTNE VGNDYNVADS KAFEGKVVDL GGSYGIRFAI VN

Macromolecule #54: HEME-A

• Macromolecule: Name: HEME-A / type: ligand / ID: 54 / Number of copies: 4 / Formula: HEA
• Molecular weight: Theoretical: 852.837 Da

Chemical component information

ChemComp-HEA:
HEME-A / Heme A

Macromolecule #55: COPPER (II) ION

• Macromolecule: Name: COPPER (II) ION / type: ligand / ID: 55 / Number of copies: 2 / Formula: CU
• Molecular weight: Theoretical: 63.546 Da

Chemical component information

ChemComp-CU:
COPPER (II) ION / Copper

Macromolecule #56: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #57: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 57 / Number of copies: 118 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

Macromolecule #58: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 58 / Number of copies: 54 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

Macromolecule #59: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• Macromolecule: Name: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 59 / Number of copies: 34 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

Macromolecule #60: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE

• Macromolecule: Name: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE; type: ligand / ID: 60 / Number of copies: 8 / Formula: LPP
• Molecular weight: Theoretical: 648.891 Da

Chemical component information

ChemComp-LPP:
2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipid*YM

Macromolecule #61: CALCIUM ION

• Macromolecule: Name: CALCIUM ION / type: ligand / ID: 61 / Number of copies: 2 / Formula: CA
• Molecular weight: Theoretical: 40.078 Da

Macromolecule #62: DINUCLEAR COPPER ION

• Macromolecule: Name: DINUCLEAR COPPER ION / type: ligand / ID: 62 / Number of copies: 2 / Formula: CUA
• Molecular weight: Theoretical: 127.092 Da

Chemical component information

ChemComp-CUA:
DINUCLEAR COPPER ION

Macromolecule #63: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 63 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #64: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 64 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #65: Ubiquinone-8

• Macromolecule: Name: Ubiquinone-8 / type: ligand / ID: 65 / Number of copies: 8 / Formula: UQ8
• Molecular weight: Theoretical: 727.109 Da

Chemical component information

ChemComp-UQ8:
Ubiquinone-8

Macromolecule #66: PROTOPORPHYRIN IX CONTAINING FE

• Macromolecule: Name: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 66 / Number of copies: 2 / Formula: HEM
• Molecular weight: Theoretical: 616.487 Da

Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

Macromolecule #67: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 67 / Number of copies: 4 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Macromolecule #68: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 68 / Number of copies: 2 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.6 µm / Nominal defocus min: 0.6 µm
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 25.66 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 138746