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-Structure paper
Title | Structural insights into preinitiation complex assembly on core promoters. |
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Journal, issue, pages | Science, Vol. 372, Issue 6541, Year 2021 |
Publish date | Apr 30, 2021 |
![]() | Xizi Chen / Yilun Qi / Zihan Wu / Xinxin Wang / Jiabei Li / Dan Zhao / Haifeng Hou / Yan Li / Zishuo Yu / Weida Liu / Mo Wang / Yulei Ren / Ze Li / Huirong Yang / Yanhui Xu / ![]() |
PubMed Abstract | Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the ...Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters. |
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Methods | EM (single particle) |
Resolution | 2.77 - 19.3 Å |
Structure data | EMDB-31075, PDB-7edx: EMDB-31107, PDB-7eg7: EMDB-31108, PDB-7eg8: EMDB-31109, PDB-7eg9: EMDB-31110, PDB-7ega: EMDB-31111, PDB-7egb: EMDB-31112, PDB-7egc: EMDB-31113, PDB-7egd: EMDB-31114, PDB-7ege: EMDB-31115, PDB-7egf: EMDB-31116, PDB-7egg: EMDB-31117, PDB-7egh: EMDB-31118, PDB-7egi: EMDB-31119, PDB-7egj: ![]() EMDB-31120: ![]() EMDB-31121: ![]() EMDB-31122: ![]() EMDB-31123: ![]() EMDB-31124: ![]() EMDB-31125: ![]() EMDB-31126: ![]() EMDB-31127: ![]() EMDB-31128: ![]() EMDB-31129: ![]() EMDB-31130: ![]() EMDB-31131: ![]() EMDB-31132: ![]() EMDB-31133: ![]() EMDB-31134: |
Chemicals | ![]() ChemComp-ZN: ![]() ChemComp-MG: ![]() ChemComp-SF4: |
Source |
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![]() | TRANSCRIPTION / TFIID / preinitiation complex / core promoter / transcription initiation / TRANSLATION |