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Structure paper

TitleFast native-SAD phasing for routine macromolecular structure determination.
Journal, issue, pagesNat. Methods, Vol. 12, Page 131-133, Year 2015
Publish dateMay 2, 2014 (structure data deposition date)
AuthorsWeinert, T. / Olieric, V. / Waltersperger, S. / Panepucci, E. / Chen, L. / Zhang, H. / Zhou, D. / Rose, J. / Ebihara, A. / Kuramitsu, S. ...Weinert, T. / Olieric, V. / Waltersperger, S. / Panepucci, E. / Chen, L. / Zhang, H. / Zhou, D. / Rose, J. / Ebihara, A. / Kuramitsu, S. / Li, D. / Howe, N. / Schnapp, G. / Pautsch, A. / Bargsten, K. / Prota, A.E. / Surana, P. / Kottur, J. / Nair, D.T. / Basilico, F. / Cecatiello, V. / Pasqualato, S. / Boland, A. / Weichenrieder, O. / Wang, B.C. / Steinmetz, M.O. / Caffrey, M. / Wang, M.
External linksNat. Methods / PubMed:25506719
MethodsX-ray diffraction
Resolution2.133 - 2.704 Å
Structure data

PDB-4pgo:
Crystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4pii:
Crystal structure of hypothetical protein PF0907 from pyrococcus furiosus solved by sulfur SAD using Swiss light source data
Method: X-RAY DIFFRACTION / Resolution: 2.17 Å

PDB-4r8t:
Structure of JEV protease
Method: X-RAY DIFFRACTION / Resolution: 2.133 Å

PDB-4r8u:
S-SAD structure of DINB-DNA Complex
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4tn8:
Crystal structure of Thermus Thermophilus thioredoxin solved by sulfur SAD using Swiss Light Source data
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4tno:
Hypothetical protein PF1117 from Pyrococcus Furiosus: Structure solved by sulfur-SAD using Swiss Light Source Data
Method: X-RAY DIFFRACTION / Resolution: 2.14 Å

PDB-4wab:
Crystal structure of mPGES1 solved by native-SAD phasing
Method: X-RAY DIFFRACTION / Resolution: 2.704 Å

PDB-4wau:
Crystal structure of CENP-M solved by native-SAD phasing
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4wbq:
Crystal structure of the exonuclease domain of QIP (QDE-2 interacting protein) solved by native-SAD phasing.
Method: X-RAY DIFFRACTION / Resolution: 2.693 Å

PDB-4wbx:
Conserved hypothetical protein PF1771 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data
Method: X-RAY DIFFRACTION / Resolution: 2.301 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-IMD:
IMIDAZOLE

ChemComp-MG:
Unknown entry

ChemComp-1FZ:
5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]thymidine

ChemComp-GSH:
GLUTATHIONE

ChemComp-LVJ:
2-[[2,6-bis(chloranyl)-3-[(2,2-dimethylpropanoylamino)methyl]phenyl]amino]-1-methyl-6-(2-methyl-2-oxidanyl-propoxy)-N-[2,2,2-tris(fluoranyl)ethyl]benzimidazole-5-carboxamide

ChemComp-CA:
Unknown entry

Source
  • pyrococcus furiosus (archaea)
  • japanese encephalitis virus
  • escherichia coli k-12 (bacteria)
  • synthetic construct (others)
  • thermus thermophilus (bacteria)
  • homo sapiens (human)
  • neurospora crassa (fungus)
KeywordsUNKNOWN FUNCTION / Sulfur SAD / SOUTHEAST COLLABORATORY FOR STRUCTURAL GENOMICS / SECSG / STRUCTURAL GENOMICS / HYDROLASE / Serine protease / Protease / NS2b / TRANSFERASE/DNA / nucleotidyl transferase / DNA Polymerase / TRANSFERASE-DNA complex / ELECTRON TRANSPORT / ISOMERASE / native-SAD / CANCER / DRUG TARGET / IN MESO CRYSTALLIZATION / INFLAMMATION / INHIBITOR / LEUKOTRIENE C4 SYNTHASE / LIPID METABOLISM / MEMBRANE-ASSOCIATED PROTEINS IN EICOSANOID AND GLUTATHIONE METABOLISM / MAPAG / MEMBRANE PROTEIN / MPGES1 / PAIN / MICROCRYSTAL / ANOMALOUS DISPERSION / SULFUR-SAD / S-SAD / CELL CYCLE / Mitosis / Kinetochore / CCAN / G-protein / RNA BINDING PROTEIN / OXIDOREDUCTASE / case studies / Swiss Light Source

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