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TitleStructure-derived insights from blood factors binding to the surfaces of different adenoviruses.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 9768, Year 2024
Publish dateNov 11, 2024
AuthorsHaley E Mudrick / Shao-Chia Lu / Janarjan Bhandari / Mary E Barry / Jack R Hemsath / Felix G M Andres / Olivia X Ma / Michael A Barry / Vijay S Reddy /
PubMed AbstractThe tropism of adenoviruses (Ads) is significantly influenced by the binding of various blood factors. To investigate differences in their binding, we conducted cryo-EM analysis on complexes of ...The tropism of adenoviruses (Ads) is significantly influenced by the binding of various blood factors. To investigate differences in their binding, we conducted cryo-EM analysis on complexes of several human adenoviruses with human platelet factor-4 (PF4), coagulation factors FII (Prothrombin), and FX. While we observed EM densities for FII and FX bound to all the species-C adenoviruses examined, no densities were seen for PF4, even though PF4 can co-pellet with various Ads. Similar to FX, the γ-carboxyglutamic acid (Gla) domain of FII binds within the surface cavity of hexon trimers. While FII binds equally to species-C Ads: Ad5, Ad6, and Ad657, FX exhibits significantly better binding to Ad5 and Ad657 compared to Ad6. Although only the FX-Gla domain is observed at high-resolution (3.7 Å), the entire FX is visible at low-resolution bound to Ad5 in three equivalent binding modes consistent with the 3-fold symmetric hexon. Only the Gla and kringle-1 domains of FII are visible on all the species-C adenoviruses, where the rigid FII binds in an upright fashion, in contrast to the flexible and bent FX. These data suggest that differential binding of FII and FX may shield certain species-C adenoviruses differently against immune molecules, thereby modulating their tropism.
External linksNat Commun / PubMed:39528527 / PubMed Central
MethodsEM (single particle)
Resolution3.61 - 5.01 Å
Structure data

45675

9cli

EMDB-45675, PDB-9cli:
Cryo-EM model derived from localized reconstruction of human adenovirus (Ad5)-hexon-FX complex at 3.6A resolution
Method: EM (single particle) / Resolution: 3.61 Å

45726

9cln

EMDB-45726, PDB-9cln:
Cryo-EM model derived from localized reconstruction of human adenovirus 5 (Ad5)-hexon-FII complex at 3.9A resolution
Method: EM (single particle) / Resolution: 4.13 Å

45729

9cls

EMDB-45729, PDB-9cls:
Cryo-EM model derived from localized reconstruction of human adenovirus 6 (Ad6)-hexon-FII complex
Method: EM (single particle) / Resolution: 3.7 Å

45737

9cm2

EMDB-45737, PDB-9cm2:
Cryo-EM model derived from localized reconstruction of human adenovirus 6-hexon-FX complex at 4.3A resolution
Method: EM (single particle) / Resolution: 5.01 Å

45744

9cm9

EMDB-45744, PDB-9cm9:
Cryo-EM model derived from localized reconstruction of Ad657-hexon-FX complex at 3.86A resolution
Method: EM (single particle) / Resolution: 4.0 Å

45751

9cmo

EMDB-45751, PDB-9cmo:
Cryo-EM model derived from localized reconstruction of Ad657-hexon-FII complex at 4.14A resolution
Method: EM (single particle) / Resolution: 4.17 Å

EMDB-46615: Human adenovirus 5 (Ad5) in complex with coagulation Factor II (Prothrombin) at 4.13 resolution.
Method: EM (single particle) / Resolution: 4.13 Å

EMDB-46620: Human adenovirus 6 (Ad6) in complex with coagulation Factor FX at 5.0A resolution.
Method: EM (single particle) / Resolution: 5.0 Å

EMDB-46654: Human Adenovirus 6 (Ad6) and Coagulation Factor II (Prothrombin) complex at 3.7A resolution
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-46660: Human Adenovirus 657 (Ad657) and Coagulation Factor X (FX)complex at 4.0A resolution
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-46770: Human Adenovirus 657 (Ad657) and Coagulation factor II (Prothrombin) at 4.17A resolution.
Method: EM (single particle) / Resolution: 4.17 Å

EMDB-51372: Human Adenovirus 5 (Ad5) and Coagulation Factor X (FX) complex at 3.6A resolution.
Method: EM (single particle) / Resolution: 3.61 Å

Chemicals

ChemComp-CA:
Unknown entry

Source
  • human adenovirus 5
  • human blood metagenome (others)
  • homo sapiens (human)
  • human adenovirus 6
  • Human adenovirus 57
KeywordsVIRUS / Adenovirus / Hexon / Coagulation factor X / Coagulation factor II / Prothrombin / Complex / Interactions / VIRAL PROTEIN

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