EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	In-cell structural insight into the stability of sperm microtubule doublet.
ジャーナル・号・ページ	Cell Discov, Vol. 9, Issue 1, Page 116, Year 2023
掲載日	2023年11月21日
著者	Linhua Tai / Guoliang Yin / Xiaojun Huang / Fei Sun / Yun Zhu /
PubMed 要旨	The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. ...The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies.
リンク	Cell Discov / PubMed:37989994 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	4.5 - 14.08 Å
構造データ	EMDB-35210: In situ structure of A-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 4.5 Å EMDB-35211: In situ structure of B-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 6.5 Å EMDB-35222: A1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 6.5 Å EMDB-35224: A2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 6.6 Å EMDB-35225: A3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 6.6 Å EMDB-35226: B1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å EMDB-35227: B2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å EMDB-35228: B3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.5 Å 35229 8i7o EMDB-35229, PDB-8i7o: In situ structure of axonemal doublet microtubules in mouse sperm with 16-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 4.5 Å 35230 8i7r EMDB-35230, PDB-8i7r: In situ structure of axonemal doublet microtubules in mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 6.5 Å EMDB-35231: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.7 Å EMDB-35232: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.7 Å EMDB-35236: Axonemal doublet microtubules in mouse sperm with 96-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.7 Å EMDB-35237: Axonemal doublet microtubules in deformed mouse sperm with 16-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 7.9 Å EMDB-35238: Axonemal doublet microtubules in deformed mouse sperm with 48-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 8.6 Å EMDB-37643: Consensus map of axonemal doublet microtubules in mouse sperm with 96-nm repeat 手法: EM (サブトモグラム平均) / 解像度: 14.08 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	mus musculus (ハツカネズミ)
キーワード	STRUCTURAL PROTEIN / microtubules / axoneme / sperm / filament