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-Structure paper
タイトル | Arrangement and symmetry of the fungal E3BP-containing core of the pyruvate dehydrogenase complex. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 4667, Year 2020 |
掲載日 | 2020年9月16日 |
![]() | B O Forsberg / S Aibara / R J Howard / N Mortezaei / E Lindahl / ![]() ![]() |
PubMed 要旨 | The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) ...The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) of mammalian PDC, PX selectively recruits E3 to the fungal PDC, but its divergent sequence suggests a distinct structural mechanism. Here, we report reconstructions of PDC from the filamentous fungus Neurospora crassa by cryo-electron microscopy, where we find protein X (PX) interior to the PDC core as opposed to substituting E2 core subunits as in mammals. Steric occlusion limits PX binding, resulting in predominantly tetrahedral symmetry, explaining previous observations in Saccharomyces cerevisiae. The PX-binding site is conserved in (and specific to) fungi, and complements possible C-terminal binding motifs in PX that are absent in mammalian E3BP. Consideration of multiple symmetries thus reveals a differential structural basis for E3BP-like function in fungal PDC. |
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手法 | EM (単粒子) |
解像度 | 2.9 - 4.4 Å |
構造データ | ![]() EMDB-11266: ![]() EMDB-11267: EMDB-11268: E2 core of the fungal Pyruvate dehydrogenase complex with flexible/oversym. periphery, structured core and S4Y-structured interior. ![]() EMDB-11269: EMDB-11270, PDB-6zlo: ![]() EMDB-11271: |
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![]() | TRANSFERASE / acetyl transferase / pyruvate dehydrogenase / protein complex / mitochondria / metabolism / tetrahedral icosahedral |