EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and mechanism of DNA delivery of a gene transfer agent.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 3034, Year 2020
掲載日	2020年6月15日
著者	Pavol Bárdy / Tibor Füzik / Dominik Hrebík / Roman Pantůček / J Thomas Beatty / Pavel Plevka /
PubMed 要旨	Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the ...Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.
リンク	Nat Commun / PubMed:32541663 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	3.32 - 10.47 Å
構造データ	10442 6tb9 EMDB-10442, PDB-6tb9: Capsid of native GTA particle computed with C5 symmetry 手法: EM (単粒子) / 解像度: 3.56 Å 10443 6tba EMDB-10443, PDB-6tba: Virion of native gene transfer agent (GTA) particle 手法: EM (単粒子) / 解像度: 4.54 Å 10476 6te8 EMDB-10476, PDB-6te8: Neck of native GTA particle computed with C12 symmetry 手法: EM (単粒子) / 解像度: 3.32 Å 10477 6te9 EMDB-10477, PDB-6te9: Neck of native GTA particle computed with C6 symmetry 手法: EM (単粒子) / 解像度: 3.58 Å 10478 6tea EMDB-10478, PDB-6tea: Tail of native GTA particle computed with helical refinement, C6 symmetry 手法: EM (らせん対称) / 解像度: 3.89 Å 10479 6teb EMDB-10479, PDB-6teb: Tail-baseplate interface of native GTA particle computed with C6 symmetry 手法: EM (単粒子) / 解像度: 4.14 Å 10490 6teh EMDB-10490, PDB-6teh: Baseplate of native GTA particle computed with C3 symmetry 手法: EM (単粒子) / 解像度: 3.99 Å 10541 6to8 EMDB-10541, PDB-6to8: Neck of empty GTA particle computed with C12 symmetry 手法: EM (単粒子) / 解像度: 3.36 Å 10542 6toa EMDB-10542, PDB-6toa: Neck of empty GTA particle computed with C6 symmetry 手法: EM (単粒子) / 解像度: 3.47 Å 10565 6tsu EMDB-10565, PDB-6tsu: Capsid of empty GTA particle computed with C5 symmetry 手法: EM (単粒子) / 解像度: 3.42 Å 10566 6tsv EMDB-10566, PDB-6tsv: Tail of empty GTA particle computed with helical refinement, C6 symmetry 手法: EM (らせん対称) / 解像度: 3.78 Å 10567 6tsw EMDB-10567, PDB-6tsw: Isometric capsid of empty GTA particle computed with I4(I,n25r) symmetry 手法: EM (単粒子) / 解像度: 4.03 Å EMDB-10568: Capsid-neck interface of native GTA particle, asymmetric reconstruction 手法: EM (単粒子) / 解像度: 4.25 Å EMDB-10569: Capsid-neck interface of empty GTA particle, asymmetric reconstruction 手法: EM (単粒子) / 解像度: 4.15 Å EMDB-10570: Tail tube of native GTA particle computed with C3 symmetry 手法: EM (単粒子) / 解像度: 4.54 Å EMDB-10571: Baseplate of empty GTA particle computed with C3 symmetry 手法: EM (単粒子) / 解像度: 4.49 Å EMDB-10572: Baseplate of native GTA particle, asymmetric reconstruction 手法: EM (単粒子) / 解像度: 4.56 Å 10592 6tui EMDB-10592, PDB-6tui: Virion of empty GTA particle 手法: EM (単粒子) / 解像度: 10.47 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER
由来	rhodobacter capsulatus (バクテリア) rhodobacter capsulatus sb 1003 (バクテリア) rhodobacter capsulatus de442 (バクテリア)
キーワード	VIRUS / "capsid" / "gene transfer agent" / "bacteriophage" / "HK97" / "virion" / "horizontal gene transfer" / "gene delivery" / "portal" / "adaptor" / "neck" / "tail tube" / "helical refinement" / "tape measure protein" / "distal tail protein" / "oligosaccharide-binding fold" / "tail tube protein" / "baseplate" / "tail" / "hub" / "connector" / "jelly roll" / "spike" / "mutant" / "variant" / "genome release"