EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function.
ジャーナル・号・ページ	Nature, Vol. 580, Issue 7805, Page 669-672, Year 2020
掲載日	2020年4月22日
著者	Svetlana O Dodonova / Fangjie Zhu / Christian Dienemann / Jussi Taipale / Patrick Cramer /
PubMed 要旨	'Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions ...'Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-renewal of embryonic stem cells. Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally distort DNA at superhelical location 2. The factors also facilitate detachment of terminal nucleosomal DNA from the histone octamer, which increases DNA accessibility. SOX-factor binding to the nucleosome can also lead to a repositioning of the N-terminal tail of histone H4 that includes residue lysine 16. We speculate that this repositioning is incompatible with higher-order nucleosome stacking, which involves contacts of the H4 tail with a neighbouring nucleosome. Our results indicate that pioneer transcription factors can use binding energy to initiate chromatin opening, and thereby facilitate nucleosome remodelling and subsequent transcription.
リンク	Nature / PubMed:32350470
手法	EM (単粒子) / X線回折
解像度	2.504 - 5.1 Å
構造データ	10390 6t79 EMDB-10390, PDB-6t79: Structure of a human nucleosome at 3.2 A resolution 手法: EM (単粒子) / 解像度: 3.2 Å 10391 6t7a EMDB-10391, PDB-6t7a: Structure of human Sox11 transcription factor in complex with a nucleosome 手法: EM (単粒子) / 解像度: 3.7 Å 10392 6t7b EMDB-10392, PDB-6t7b: Structure of human Sox2 transcription factor in complex with a nucleosome 手法: EM (単粒子) / 解像度: 5.1 Å 10393 6t7c EMDB-10393, PDB-6t7c: Structure of two copies of human Sox11 transcription factor in complex with a nucleosome 手法: EM (単粒子) / 解像度: 4.0 Å 10394 6t7d EMDB-10394, PDB-6t7d: Structure of human Sox11 transcription factor in complex with a nucleosome 手法: EM (単粒子) / 解像度: 4.4 Å PDB-6t78: Structure of human Sox11 transcription factor in complex with a short DNA fragment 手法: X-RAY DIFFRACTION / 解像度: 2.504 Å
化合物	ChemComp-HOH: WATER
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	NUCLEAR PROTEIN / Sox11 / transcription factor / pioneer factor / DNA / Nucleosome / histones / Sox2