EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM shows how dynactin recruits two dyneins for faster movement.
ジャーナル・号・ページ	Nature, Vol. 554, Issue 7691, Page 202-206, Year 2018
掲載日	2018年2月7日
著者	Linas Urnavicius / Clinton K Lau / Mohamed M Elshenawy / Edgar Morales-Rios / Carina Motz / Ahmet Yildiz / Andrew P Carter /
PubMed 要旨	Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. ...Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. Here we use electron microscopy and single-molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased towards recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two dyneins. We find that the shift towards a double dynein complex increases both the force and speed of the microtubule motor. Our 3.5 Å resolution cryo-electron microscopy reconstruction of a dynein tail-dynactin-BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side. Our work provides a structural basis for understanding how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dynein-dynactin transport machine.
リンク	Nature / PubMed:29420470 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.79 - 8.2 Å
構造データ	4168 6f1t EMDB-4168, PDB-6f1t: Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1 手法: EM (単粒子) / 解像度: 3.5 Å 4169 6f1u EMDB-4169, PDB-6f1u: N terminal region of dynein tail domains in complex with dynactin filament and BICDR-1 手法: EM (単粒子) / 解像度: 3.4 Å 4170 6f1v EMDB-4170, PDB-6f1v: C terminal region of the dynein heavy chains in the dynein tail/dynactin/BICDR1 complex 手法: EM (単粒子) / 解像度: 3.4 Å 4171 6f1y EMDB-4171, PDB-6f1y: Dynein light intermediate chain region of the dynein tail/dynactin/BICDR1 complex 手法: EM (単粒子) / 解像度: 3.4 Å 4172 6f1z EMDB-4172, PDB-6f1z: Roadblock-1 region of the dynein tail/dynactin/BICDR1 complex 手法: EM (単粒子) / 解像度: 3.4 Å 4177 6f38 EMDB-4177, PDB-6f38: Cryo-EM structure of two dynein tail domains bound to dynactin and HOOK3 手法: EM (単粒子) / 解像度: 6.7 Å PDB-5owo: Human cytoplasmic Dynein N-Terminus dimerization domain at 1.8 Angstrom resolution 手法: X-RAY DIFFRACTION / 解像度: 1.79 Å PDB-6f3a: Cryo-EM structure of a single dynein tail domain bound to dynactin and BICD2N 手法: ELECTRON MICROSCOPY / 解像度: 8.2 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HOH: WATER / 水 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸
由来	mus musculus (ハツカネズミ) homo sapiens (ヒト) sus scrofa (ブタ) Pig (ブタ)
キーワード	MOTOR PROTEIN (モータータンパク質) / Dynein (ダイニン) / Heavy chain / Dimerization domain / Cryo-EM (低温電子顕微鏡法) / Complex / dynein/dynactin/BICDR / TDR / Cargo adaptor / TDH / DDH / dynein/dynactin/HOOK3 / TDB / dynein/dynactin/BICD2