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-Structure paper
タイトル | Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV. |
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ジャーナル・号・ページ | Cell, Vol. 170, Issue 6, Page 1247-1257.e12, Year 2017 |
掲載日 | 2017年9月7日 |
著者 | Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang / |
PubMed 要旨 | The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole. |
リンク | Cell / PubMed:28844695 |
手法 | EM (単粒子) |
解像度 | 3.4 - 17.4 Å |
構造データ | EMDB-6771, PDB-5xtb: EMDB-6772, PDB-5xtc: EMDB-6774, PDB-5xte: |
化合物 | ChemComp-SF4: ChemComp-FMN: ChemComp-8Q1: ChemComp-NDP: ChemComp-FES: ChemComp-PLX: ChemComp-CDL: ChemComp-PEE: ChemComp-HEC: ChemComp-HEM: ChemComp-CU: ChemComp-MG: ChemComp-HEA: ChemComp-ZN: |
由来 |
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キーワード | OXIDOREDUCTASE/ELECTRON TRANSPORT / Respiratory / OXIDOREDUCTASE-ELECTRON TRANSPORT complex / ELECTRON TRANSPORT / Homo sapiens / Oxidoreductase / Complex / Supercomplex |