EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and receptor binding activities of merbecovirus spike proteins reveal key signatures for human DPP4 adaptation.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 28, Page eadv7296, Year 2025
掲載日	2025年7月11日
著者	Hang Yuan / Jingjing Wang / Yong Ma / Zimu Li / Xijie Gao / Gul Habib / Banghui Liu / Jing Chen / Jun He / Peng Zhou / Zheng-Li Shi / Xinwen Chen / Xiaoli Xiong /
PubMed 要旨	Merbecoviruses from bats, pangolins, and hedgehogs pose significant zoonotic threats, with a limited understanding of receptor binding by their spike (S) proteins. Here, we report cryo-EM structures ...Merbecoviruses from bats, pangolins, and hedgehogs pose significant zoonotic threats, with a limited understanding of receptor binding by their spike (S) proteins. Here, we report cryo-EM structures of GD-BatCoV (BtCoV-422) and SE-PangolinCoV (MjHKU4r-CoV-1) RBDs in complex with human DPP4 (hDPP4). These structures exhibit a substantial offset in their hDPP4 interaction interfaces, revealing a conserved hydrophobic cluster as a convergent signature of DPP4 binding within the MERS-HKU4 clade of merbecoviruses. Structure-guided mutagenesis demonstrates that favorable interactions are distributed across multiple receptor binding motif (RBM) regions, working synergistically to confer high-affinity hDPP4 binding. Swapping of the merbecovirus RBM regions indicate limited plasticity and interchangeability among these regions. In addition, we report cryo-EM structures of six merbecovirus S-trimers. Structure-based phylogenetics suggests that hDPP4-binding merbecoviruses undergo convergent evolution, while ACE2-binding merbecoviruses exhibit diversification in their binding mechanisms. These findings offer critical insights into merbecovirus receptor utilization, providing a structural understanding for future surveillance.
リンク	Sci Adv / PubMed:40644548 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.8 Å
構造データ	61600 9jmf EMDB-61600, PDB-9jmf: Cryo-EM structure of SA-BatCoV (Neoromicia/PML-PHE1/RSA/2011) S-trimer 手法: EM (単粒子) / 解像度: 3.1 Å 61601 9jmg EMDB-61601, PDB-9jmg: Cryo-EM structure of EU-HedgehogCoV (Erinaceus/VMC/DEU/2012) S-trimer in a locked-2 conformation 手法: EM (単粒子) / 解像度: 3.0 Å 61602 9jmh EMDB-61602, PDB-9jmh: Cryo-EM structure of HKU25-BatCoV S-trimer stabilized with 2P and x1 disulfide bond 手法: EM (単粒子) / 解像度: 3.0 Å 61603 9jmi EMDB-61603, PDB-9jmi: Cryo-EM structure of CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) S-trimer in a locked-2 conformation 手法: EM (単粒子) / 解像度: 3.3 Å 61604 9jmj EMDB-61604, PDB-9jmj: Cryo-EM structure of the GD-BatCoV (BtCoV/Ii/GD/2014-422) RBD in complex with human DPP4 手法: EM (単粒子) / 解像度: 3.4 Å 61606 9jmm EMDB-61606, PDB-9jmm: Cryo-EM structure of the SE-PangolinCoV (MjHKU4r-CoV-1) RBD in complex with human DPP4 手法: EM (単粒子) / 解像度: 2.8 Å 61607 9jmn EMDB-61607, PDB-9jmn: Cryo-EM structure of CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) S-trimer in a locked-1 conformation 手法: EM (単粒子) / 解像度: 3.8 Å 61608 9jmo EMDB-61608, PDB-9jmo: Cryo-EM structure of Japan-BatCoV (Vs-CoV-1) S-trimer 手法: EM (単粒子) / 解像度: 2.8 Å 61609 9jmp EMDB-61609, PDB-9jmp: Cryo-EM structure of GD-BatCoV (BtCoV/Ii/GD/2014-422) S-trimer 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-EIC: LINOLEIC ACID ChemComp-FOL: FOLIC ACID
由来	coronavirus neoromicia/pml-phe1/rsa/2011 (ウイルス) enterobacteria phage t4 (ファージ) betacoronavirus erinaceus/vmc/deu/2012 (ウイルス) enterobacteria phage t4 (bacteriophage t4) (ウイルス) hypsugo bat coronavirus hku25 (ウイルス) tequatrovirus t4 (ウイルス) erinaceus hedgehog coronavirus hku31 (ウイルス) middle east respiratory syndrome-related coronavirus (ウイルス) homo sapiens (ヒト) pangolin coronavirus hku4/p251t/pangolin/2018 (ウイルス) bat coronavirus (ウイルス)
キーワード	VIRAL PROTEIN / spike protein / complex