EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and functional mechanisms of anti-NMDAR autoimmune encephalitis.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年9月3日
著者	Kevin Michalski / Taha Abdulla / Sam Kleeman / Lars Schmidl / Ricardo Gómez / Noriko Simorowski / Francesca Vallese / Harald Prüss / Manfred Heckmann / Christian Geis / Hiro Furukawa /
PubMed 要旨	Autoantibodies against neuronal membrane proteins can manifest in autoimmune encephalitis, inducing seizures, cognitive dysfunction and psychosis. Anti-N-methyl-D-aspartate receptor (NMDAR) ...Autoantibodies against neuronal membrane proteins can manifest in autoimmune encephalitis, inducing seizures, cognitive dysfunction and psychosis. Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is the most dominant autoimmune encephalitis; however, insights into how autoantibodies recognize and alter receptor functions remain limited. Here we determined structures of human and rat NMDARs bound to three distinct patient-derived antibodies using single-particle electron cryo-microscopy. These antibodies bind different regions within the amino-terminal domain of the GluN1 subunit. Through electrophysiology, we show that all three autoantibodies acutely and directly reduced NMDAR channel functions in primary neurons. Antibodies show different stoichiometry of binding and antibody-receptor complex formation, which in one antibody, 003-102, also results in reduced synaptic localization of NMDARs. These studies demonstrate mechanisms of diverse epitope recognition and direct channel regulation of anti-NMDAR autoantibodies underlying autoimmune encephalitis.
リンク	Nat Struct Mol Biol / PubMed:39227719
手法	EM (単粒子)
解像度	3.69 - 4.42 Å
構造データ	43530 8vuh EMDB-43530, PDB-8vuh: Human GluN1-2A IgG 003-102 splayed conformation 手法: EM (単粒子) / 解像度: 4.42 Å 43531 8vuj EMDB-43531, PDB-8vuj: Human GluN1-2A with Fab 003-102 手法: EM (単粒子) / 解像度: 3.92 Å 43532 8vul EMDB-43532, PDB-8vul: Human GluN1-2A with Fab 003-102 Local refinement of ATD 手法: EM (単粒子) / 解像度: 3.83 Å 43534 8vun EMDB-43534, PDB-8vun: Human GluN1-2A With Fab 008-218 手法: EM (単粒子) / 解像度: 4.01 Å 43536 8vuq EMDB-43536, PDB-8vuq: Human GluN1-2A with Fab 008-218 Local refinement of ATD 手法: EM (単粒子) / 解像度: 3.85 Å 43537 8vur EMDB-43537, PDB-8vur: Human GluN1-2A with IgG 003-102 WT conformation 手法: EM (単粒子) / 解像度: 3.84 Å 43538 8vus EMDB-43538, PDB-8vus: Human GluN1-2A with IgG 007-168 手法: EM (単粒子) / 解像度: 3.99 Å 43539 8vut EMDB-43539, PDB-8vut: Human GluN1-2A with IgG 008-218 手法: EM (単粒子) / 解像度: 3.7 Å 43540 8vuu EMDB-43540, PDB-8vuu: Human GluN1-2B with Fab 007-168 手法: EM (単粒子) / 解像度: 4.05 Å 43541 8vuv EMDB-43541, PDB-8vuv: Human GluN1-2B with Fab 007-168 Local refinement of ATD 手法: EM (単粒子) / 解像度: 3.69 Å 43544 8vuy EMDB-43544, PDB-8vuy: Rat GluN1-2B with Fab 003-102 手法: EM (単粒子) / 解像度: 3.81 Å 43559 8vvh EMDB-43559, PDB-8vvh: rat GluN1a-2B Fab 003-102 local refinement 手法: EM (単粒子) / 解像度: 3.95 Å
由来	homo sapiens (ヒト) rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE/IMMUNE SYSTEM / Receptor / antibody / ion channel / MEMBRANE-IMMUNE SYSTEM complex / MEMBRANE PROTEIN/IMMUNE SYSTEM / Channel / heterotetramer / MEMBRANE PROTEIN-IMMUNE SYSTEM complex / MEMBRANE PROTEIN