EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into preinitiation complex assembly on core promoters.
ジャーナル・号・ページ	Science, Vol. 372, Issue 6541, Year 2021
掲載日	2021年4月30日
著者	Xizi Chen / Yilun Qi / Zihan Wu / Xinxin Wang / Jiabei Li / Dan Zhao / Haifeng Hou / Yan Li / Zishuo Yu / Weida Liu / Mo Wang / Yulei Ren / Ze Li / Huirong Yang / Yanhui Xu /
PubMed 要旨	Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the ...Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.
リンク	Science / PubMed:33795473
手法	EM (単粒子)
解像度	2.77 - 19.3 Å
構造データ	31075 7edx EMDB-31075, PDB-7edx: p53-bound TFIID-based core PIC on HDM2 promoter 手法: EM (単粒子) / 解像度: 4.5 Å 31107 7eg7 EMDB-31107, PDB-7eg7: TFIID-based core PIC on SCP promoter 手法: EM (単粒子) / 解像度: 6.2 Å 31108 7eg8 EMDB-31108, PDB-7eg8: TFIID-based core PIC on PUMA promoter 手法: EM (単粒子) / 解像度: 7.4 Å 31109 7eg9 EMDB-31109, PDB-7eg9: TFIID-based intermediate PIC on SCP promoter 手法: EM (単粒子) / 解像度: 3.7 Å 31110 7ega EMDB-31110, PDB-7ega: TFIID-based intermediate PIC on PUMA promoter 手法: EM (単粒子) / 解像度: 4.1 Å 31111 7egb EMDB-31111, PDB-7egb: TFIID-based holo PIC on SCP promoter 手法: EM (単粒子) / 解像度: 3.3 Å 31112 7egc EMDB-31112, PDB-7egc: p53-bound TFIID-based holo PIC on HDM2 promoter 手法: EM (単粒子) / 解像度: 3.9 Å 31113 7egd EMDB-31113, PDB-7egd: SCP promoter-bound TFIID-TFIIA in initial TBP-loading state 手法: EM (単粒子) / 解像度: 6.75 Å 31114 7ege EMDB-31114, PDB-7ege: TFIID in canonical conformation 手法: EM (単粒子) / 解像度: 9.0 Å 31115 7egf EMDB-31115, PDB-7egf: TFIID lobe A subcomplex 手法: EM (単粒子) / 解像度: 3.16 Å 31116 7egg EMDB-31116, PDB-7egg: TFIID lobe B subcomplex 手法: EM (単粒子) / 解像度: 2.77 Å 31117 7egh EMDB-31117, PDB-7egh: TFIID lobe C subcomplex 手法: EM (単粒子) / 解像度: 3.04 Å 31118 7egi EMDB-31118, PDB-7egi: TFIID in rearranged conformation 手法: EM (単粒子) / 解像度: 9.82 Å 31119 7egj EMDB-31119, PDB-7egj: SCP promoter-bound TFIID-TFIIA in post TBP-loading state 手法: EM (単粒子) / 解像度: 8.64 Å EMDB-31120: TFIID-based core PIC on RPLP1 promoter 手法: EM (単粒子) / 解像度: 15.6 Å EMDB-31121: TFIID-based core PIC on SCP+3 promoter 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-31122: TFIID-based core PIC on SCP+6 promoter 手法: EM (単粒子) / 解像度: 12.7 Å EMDB-31123: TFIID-based core PIC on PUMA-10 promoter 手法: EM (単粒子) / 解像度: 7.7 Å EMDB-31124: TFIID-based core PIC on SCP+10 promoter 手法: EM (単粒子) / 解像度: 5.72 Å EMDB-31125: TFIID-based core PIC on CLAM2 promoter 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-31126: TFIID-based core PIC on POLB promoter 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-31127: TFIID-based core PIC on JUNB promoter 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-31128: TFIID-based core PIC on TAF7 promoter 手法: EM (単粒子) / 解像度: 19.3 Å EMDB-31129: TFIID-based core PIC on PUMA-GC promoter 手法: EM (単粒子) / 解像度: 19.1 Å EMDB-31130: TFIID-based holo PIC on PUMA promoter 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-31131: TFIID-based holo PIC on SCP promoter in pre-assembled state 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-31132: 3.5 Angstrom CAK module 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-31133: PUMA promoter-bound TFIID-TFIIA 手法: EM (単粒子) / 解像度: 7.85 Å EMDB-31134: HDM2 promoter-bound TFIID-TFIIA 手法: EM (単粒子) / 解像度: 9.77 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SF4: IRON/SULFUR CLUSTER
由来	homo sapiens (ヒト) sus scrofa (ブタ) pig (ブタ) human (ヒト)
キーワード	TRANSCRIPTION / TFIID / preinitiation complex / core promoter / transcription initiation / TRANSLATION