EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年8月4日
著者	George Ueda / Aleksandar Antanasijevic / Jorge A Fallas / William Sheffler / Jeffrey Copps / Daniel Ellis / Geoffrey B Hutchinson / Adam Moyer / Anila Yasmeen / Yaroslav Tsybovsky / Young-Jun Park / Matthew J Bick / Banumathi Sankaran / Rebecca A Gillespie / Philip Jm Brouwer / Peter H Zwart / David Veesler / Masaru Kanekiyo / Barney S Graham / Rogier W Sanders / John P Moore / Per Johan Klasse / Andrew B Ward / Neil P King / David Baker /
PubMed 要旨	Multivalent presentation of viral glycoproteins can substantially increase the elicitation of antigen-specific antibodies. To enable a new generation of anti-viral vaccines, we designed self- ...Multivalent presentation of viral glycoproteins can substantially increase the elicitation of antigen-specific antibodies. To enable a new generation of anti-viral vaccines, we designed self-assembling protein nanoparticles with geometries tailored to present the ectodomains of influenza, HIV, and RSV viral glycoprotein trimers. We first designed trimers tailored for antigen fusion, featuring N-terminal helices positioned to match the C termini of the viral glycoproteins. Trimers that experimentally adopted their designed configurations were incorporated as components of tetrahedral, octahedral, and icosahedral nanoparticles, which were characterized by cryo-electron microscopy and assessed for their ability to present viral glycoproteins. Electron microscopy and antibody binding experiments demonstrated that the designed nanoparticles presented antigenically intact prefusion HIV-1 Env, influenza hemagglutinin, and RSV F trimers in the predicted geometries. This work demonstrates that antigen-displaying protein nanoparticles can be designed from scratch, and provides a systematic way to investigate the influence of antigen presentation geometry on the immune response to vaccination.
リンク	Elife / PubMed:32748788 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.303 - 34.17 Å
構造データ	EMDB-21162: De novo designed tetrahedral nanoparticle T33_dn2 手法: EM (単粒子) / 解像度: 17.11 Å EMDB-21163: De novo designed tetrahedral nanoparticle T33_dn5 手法: EM (単粒子) / 解像度: 19.68 Å EMDB-21164: De novo designed tetrahedral nanoparticle T33_dn10 手法: EM (単粒子) / 解像度: 19.13 Å EMDB-21165: De novo designed octahedral nanoparticle O43_dn18 手法: EM (単粒子) / 解像度: 16.4 Å EMDB-21166: De novo designed icosahedral nanoparticle I53_dn5 手法: EM (単粒子) / 解像度: 18.74 Å EMDB-21167: BG505-SOSIP-T33_dn2A nanoparticle fusion component 手法: EM (単粒子) / 解像度: 15.62 Å EMDB-21168: BG505-SOSIP-T33_dn2A nanoparticle fusion component in complex with VRC01-Fab 手法: EM (単粒子) / 解像度: 15.62 Å EMDB-21169: De novo designed tetrahedral nanoparticle T33_dn2 presenting BG505-SOSIP 手法: EM (単粒子) / 解像度: 34.17 Å EMDB-21170: Tetrahedral nanoparticle T33_dn10 presenting BG505-SOSIP 手法: EM (単粒子) / 解像度: 25.67 Å EMDB-21171: Icosahedral Nanoparticle I53_dn5 presenting BG505-SOSIP 手法: EM (単粒子) / 解像度: 23.48 Å 21172 6vfh EMDB-21172, PDB-6vfh: De novo designed tetrahedral nanoparticle T33_dn10 手法: EM (単粒子) / 解像度: 3.86 Å 21173 6vfi EMDB-21173, PDB-6vfi: De novo designed octahedral nanoparticle O43_dn18 手法: EM (単粒子) / 解像度: 4.54 Å 21174 6vfj EMDB-21174, PDB-6vfj: De novo designed icosahedral nanoparticle I53_dn5 手法: EM (単粒子) / 解像度: 5.35 Å PDB-6v8e: Computationally designed C3-symmetric homotrimer from TPR repeat protein 手法: X-RAY DIFFRACTION / 解像度: 2.53 Å PDB-6veh: Computationally designed C3-symmetric homotrimer from HEAT repeat protein 手法: X-RAY DIFFRACTION / 解像度: 2.303 Å
化合物	ChemComp-HOH: WATER / 水
由来	synthetic construct (人工物)
キーワード	DE NOVO PROTEIN (De novo) / designed trimers / designed nanoparticles / ribosome-binding site / designed protein (デザイン) / vaccine (ワクチン) / De novo (De novo) / Nanoparticle (ナノ粒子)