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Title | Cryo-EM shows how dynactin recruits two dyneins for faster movement. |
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Journal, issue, pages | Nature, Vol. 554, Issue 7691, Page 202-206, Year 2018 |
Publish date | Feb 7, 2018 |
Authors | Linas Urnavicius / Clinton K Lau / Mohamed M Elshenawy / Edgar Morales-Rios / Carina Motz / Ahmet Yildiz / Andrew P Carter / |
PubMed Abstract | Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. ...Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. Here we use electron microscopy and single-molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased towards recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two dyneins. We find that the shift towards a double dynein complex increases both the force and speed of the microtubule motor. Our 3.5 Å resolution cryo-electron microscopy reconstruction of a dynein tail-dynactin-BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side. Our work provides a structural basis for understanding how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dynein-dynactin transport machine. |
External links | Nature / PubMed:29420470 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.79 - 8.2 Å |
Structure data | EMDB-4168, PDB-6f1t: EMDB-4169, PDB-6f1u: EMDB-4170, PDB-6f1v: EMDB-4171, PDB-6f1y: EMDB-4172, PDB-6f1z: EMDB-4177, PDB-6f38: PDB-5owo: PDB-6f3a: |
Chemicals | ChemComp-GOL: ChemComp-MG: ChemComp-CA: ChemComp-NA: ChemComp-K: ChemComp-HOH: ChemComp-ADP: ChemComp-ATP: |
Source |
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Keywords | MOTOR PROTEIN / Dynein / Heavy chain / Dimerization domain / Cryo-EM / Complex / dynein/dynactin/BICDR / TDR / Cargo adaptor / TDH / DDH / dynein/dynactin/HOOK3 / TDB / dynein/dynactin/BICD2 |