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Structure paper

TitleA cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 7257, Year 2021
Publish dateDec 14, 2021
AuthorsHongcheng Fan / Bo Wang / Yan Zhang / Yun Zhu / Bo Song / Haijin Xu / Yujia Zhai / Mingqiang Qiao / Fei Sun /
PubMed AbstractCryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution structures of biomacromolecules in solution. However, air-water interface induced preferred orientations, ...Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution structures of biomacromolecules in solution. However, air-water interface induced preferred orientations, dissociation or denaturation of biomacromolecules during cryo-vitrification remains a limiting factor for many specimens. To solve this bottleneck, we developed a cryo-EM support film using 2D crystals of hydrophobin HFBI. The hydrophilic side of the HFBI film adsorbs protein particles via electrostatic interactions and sequesters them from the air-water interface, allowing the formation of sufficiently thin ice for high-quality data collection. The particle orientation distribution can be regulated by adjusting the buffer pH. Using this support, we determined the cryo-EM structures of catalase (2.29 Å) and influenza haemagglutinin trimer (2.56 Å), which exhibited strong preferred orientations using a conventional cryo-vitrification protocol. We further show that the HFBI film is suitable to obtain high-resolution structures of small proteins, including aldolase (150 kDa, 3.28 Å) and haemoglobin (64 kDa, 3.6 Å). Our work suggests that HFBI films may have broad future applications in increasing the success rate and efficiency of cryo-EM.
External linksNat Commun / PubMed:34907237 / PubMed Central
MethodsEM (single particle)
Resolution1.96 - 3.6 Å
Structure data

31910

7vd8

EMDB-31910, PDB-7vd8:
1.96 A structure of human apoferritin obtained from Talos Arctica microscope
Method: EM (single particle) / Resolution: 1.96 Å

31911

7vd9

EMDB-31911, PDB-7vd9:
2.29 A structure of the human catalase
Method: EM (single particle) / Resolution: 2.29 Å

31912

7vda

EMDB-31912, PDB-7vda:
2.26 A structure of the glutamate dehydrogenase
Method: EM (single particle) / Resolution: 2.26 Å

31913

7vdc

EMDB-31913, PDB-7vdc:
3.28 A structure of the rabbit muscle aldolase
Method: EM (single particle) / Resolution: 3.28 Å

31915

7vde

EMDB-31915, PDB-7vde:
3.6 A structure of the human hemoglobin
Method: EM (single particle) / Resolution: 3.6 Å

31916

7vdf

EMDB-31916, PDB-7vdf:
2.56 A structure of influenza hemagglutinin (HA) trimer
Method: EM (single particle) / Resolution: 2.56 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-BMA:
beta-D-mannopyranose / Mannose

Source
  • homo sapiens (human)
  • Human (human)
  • bos taurus (cattle)
  • Bovine (cattle)
  • oryctolagus cuniculus (rabbit)
  • Rabbit (rabbit)
  • H3N2 subtype (virus)
  • influenza a virus (strain a/hong kong/1/1968 h3n2)
KeywordsSTRUCTURAL PROTEIN / Apoferritin / Catalase / Glutamate dehydrogenase / Rabbit muscle aldolase / Human hemoglobin / VIRAL PROTEIN / Influenza hemagglutinin (HA) trimer

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