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TitleStructural insights into preinitiation complex assembly on core promoters.
Journal, issue, pagesScience, Vol. 372, Issue 6541, Year 2021
Publish dateApr 30, 2021
AuthorsXizi Chen / Yilun Qi / Zihan Wu / Xinxin Wang / Jiabei Li / Dan Zhao / Haifeng Hou / Yan Li / Zishuo Yu / Weida Liu / Mo Wang / Yulei Ren / Ze Li / Huirong Yang / Yanhui Xu /
PubMed AbstractTranscription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the ...Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.
External linksScience / PubMed:33795473
MethodsEM (single particle)
Resolution2.77 - 19.3 Å
Structure data

EMDB-31075, PDB-7edx:
p53-bound TFIID-based core PIC on HDM2 promoter
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-31107, PDB-7eg7:
TFIID-based core PIC on SCP promoter
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-31108, PDB-7eg8:
TFIID-based core PIC on PUMA promoter
Method: EM (single particle) / Resolution: 7.4 Å

EMDB-31109, PDB-7eg9:
TFIID-based intermediate PIC on SCP promoter
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-31110, PDB-7ega:
TFIID-based intermediate PIC on PUMA promoter
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-31111, PDB-7egb:
TFIID-based holo PIC on SCP promoter
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-31112, PDB-7egc:
p53-bound TFIID-based holo PIC on HDM2 promoter
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-31113, PDB-7egd:
SCP promoter-bound TFIID-TFIIA in initial TBP-loading state
Method: EM (single particle) / Resolution: 6.75 Å

EMDB-31114, PDB-7ege:
TFIID in canonical conformation
Method: EM (single particle) / Resolution: 9 Å

EMDB-31115, PDB-7egf:
TFIID lobe A subcomplex
Method: EM (single particle) / Resolution: 3.16 Å

EMDB-31116, PDB-7egg:
TFIID lobe B subcomplex
Method: EM (single particle) / Resolution: 2.77 Å

EMDB-31117, PDB-7egh:
TFIID lobe C subcomplex
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-31118, PDB-7egi:
TFIID in rearranged conformation
Method: EM (single particle) / Resolution: 9.82 Å

EMDB-31119, PDB-7egj:
SCP promoter-bound TFIID-TFIIA in post TBP-loading state
Method: EM (single particle) / Resolution: 8.64 Å

EMDB-31120:
TFIID-based core PIC on RPLP1 promoter
Method: EM (single particle) / Resolution: 15.6 Å

EMDB-31121:
TFIID-based core PIC on SCP+3 promoter
Method: EM (single particle) / Resolution: 6.5 Å

EMDB-31122:
TFIID-based core PIC on SCP+6 promoter
Method: EM (single particle) / Resolution: 12.7 Å

EMDB-31123:
TFIID-based core PIC on PUMA-10 promoter
Method: EM (single particle) / Resolution: 7.7 Å

EMDB-31124:
TFIID-based core PIC on SCP+10 promoter
Method: EM (single particle) / Resolution: 5.72 Å

EMDB-31125:
TFIID-based core PIC on CLAM2 promoter
Method: EM (single particle) / Resolution: 12 Å

EMDB-31126:
TFIID-based core PIC on POLB promoter
Method: EM (single particle) / Resolution: 19 Å

EMDB-31127:
TFIID-based core PIC on JUNB promoter
Method: EM (single particle) / Resolution: 8 Å

EMDB-31128:
TFIID-based core PIC on TAF7 promoter
Method: EM (single particle) / Resolution: 19.3 Å

EMDB-31129:
TFIID-based core PIC on PUMA-GC promoter
Method: EM (single particle) / Resolution: 19.1 Å

EMDB-31130:
TFIID-based holo PIC on PUMA promoter
Method: EM (single particle) / Resolution: 6.1 Å

EMDB-31131:
TFIID-based holo PIC on SCP promoter in pre-assembled state
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-31132:
3.5 Angstrom CAK module
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-31133:
PUMA promoter-bound TFIID-TFIIA
Method: EM (single particle) / Resolution: 7.85 Å

EMDB-31134:
HDM2 promoter-bound TFIID-TFIIA
Method: EM (single particle) / Resolution: 9.77 Å

Chemicals

ChemComp-ZN:
ZINC ION / Zinc

ChemComp-MG:
MAGNESIUM ION / Magnesium

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Source
  • homo sapiens (human)
  • sus scrofa (pig)
  • Pig (pig)
  • Human (human)
KeywordsAnimals / Apoptosis Regulatory Proteins / BBC3 protein, human / Corticotropin-Releasing Hormone / Cryoelectron Microscopy / Cyclin-Dependent Kinases / HEK293 Cells / Humans / MDM2 protein, human / Multiprotein Complexes / Phosphorylation / Promoter Regions, Genetic / Protein Binding / Protein Domains / Proto-Oncogene Proteins / Proto-Oncogene Proteins c-mdm2 / RNA Polymerase II / Swine / Transcription Factor TFIID / Transcription Initiation, Genetic / UCN3 protein, human / Urocortins / TRANSCRIPTION / TFIID / preinitiation complex / core promoter / transcription initiation / TRANSLATION

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