EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 5187, Year 2020
掲載日	2020年10月14日
著者	Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts /
PubMed 要旨	Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
リンク	Nat Commun / PubMed:33056988 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.5 Å
構造データ	10958 6yw5 EMDB-10958, PDB-6yw5: The structure of the small subunit of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-10961: Cryo-EM map of the head of the small subunit of the mitoribosome from Neurospora crassa. 手法: EM (単粒子) / 解像度: 2.79 Å EMDB-10962: Cryo-EM map of the body of the small subunit of the mitoribosome from Neurospora crassa. 手法: EM (単粒子) / 解像度: 2.79 Å EMDB-10963: Cryo-EM map of the tail of the small subunit of the mitoribosome from Neurospora crassa. 手法: EM (単粒子) / 解像度: 2.86 Å 10965 6ywe EMDB-10965, PDB-6ywe: The structure of the mitoribosome from Neurospora crassa in the P/E tRNA bound state 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-10966: Cryo-EM map of the core of the large subunit of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-10967: Cryo-EM map of the L10 region of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-10968: Cryo-EM map of the CP of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-10969: Cryo-EM map of the head of the small subunit of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 2.94 Å EMDB-10970: Cryo-EM map of the body of the small subunit of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 2.94 Å EMDB-10971: Cryo-EM map of the L1 stalk region of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-10972: Cryo-EM map of the tail region of the mitoribosome from Neurospora crassa with tRNA in the P/E state 手法: EM (単粒子) / 解像度: 2.99 Å 10973 6yws EMDB-10973, PDB-6yws: The structure of the large subunit of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 2.74 Å EMDB-10974: Cryo-EM map of the core of the large subunit of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-10975: Cryo-EM map of the L10 region of the large subunit of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-10976: Cryo-EM map of the CP of the large subunit of the mitoribosome from Neurospora crassa. 手法: EM (単粒子) / 解像度: 2.88 Å 10977 6ywv EMDB-10977, PDB-6ywv: The structure of the Atp25 bound assembly intermediate of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 3.03 Å 10978 6ywx EMDB-10978, PDB-6ywx: The structure of the mitoribosome from Neurospora crassa with tRNA bound to the E-site 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-10979: Cryo-EM map of the core of the large subunit of the mitoribosome from Neurospora crassa with tRNA bound to the E-site 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-10980: Cryo-EM map of the L10 region of the mitoribosome from Neurospora crassa with tRNA bound to the E-site 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-10981: Cryo-EM map of the CP of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-10982: Cryo-EM map of the head of the small subunit of the mitoribosome from Neurospora crassa 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-10983: Cryo-EM map of the tail of the small subunit of the mitoribosome from Neurospora crassa with tRNA bound to the E-site 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-10984: Cryo-EM map of the body of the small subunit of the mitoribosome from Neurospora crassa with tRNA bound to the E-site 手法: EM (単粒子) / 解像度: 3.07 Å 10985 6ywy EMDB-10985, PDB-6ywy: The structure of the mitoribosome from Neurospora crassa with bound tRNA at the P-site 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-10986: Cryo-EM map of the core of the large subunit of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 2.97 Å EMDB-10988: Cryo-EM map of the L10 region of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-10989: Cryo-EM map of the CP of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 3.01 Å EMDB-10990: Cryo-EM map of the head of the small subunit of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-10991: Cryo-EM map of the body of the small subunit of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-10992: Cryo-EM map for the tail of the small subunit of the mitoribosome from Neurospora crassa with tRNA bound to the P-site 手法: EM (単粒子) / 解像度: 3.01 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-K: Unknown entry / カリウムカチオン ChemComp-SPM: SPERMINE / スペルミン ChemComp-ZN: Unknown entry ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM
由来	neurospora crassa or74a (菌類) neurospora crassa (菌類)
キーワード	TRANSLATION / Neurospora crassa / translating Mitoribosomes / tRNA / mRNA / mL108