|Title||Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.|
|Journal, issue, pages||Immunity, Vol. 49, Issue 2, Page 288-300.e8, Year 2018|
|Publish date||Aug 21, 2018|
|Authors||Matteo Bianchi / Hannah L Turner / Bartek Nogal / Christopher A Cottrell / David Oyen / Matthias Pauthner / Raiza Bastidas / Rebecca Nedellec / Laura E McCoy / Ian A Wilson / Dennis R Burton / Andrew B Ward / Lars Hangartner /|
|PubMed Abstract||Characterizing polyclonal antibody responses via currently available methods is inherently complex and difficult. Mapping epitopes in an immune response is typically incomplete, which creates a ...Characterizing polyclonal antibody responses via currently available methods is inherently complex and difficult. Mapping epitopes in an immune response is typically incomplete, which creates a barrier to fully understanding the humoral response to antigens and hinders rational vaccine design efforts. Here, we describe a method of characterizing polyclonal responses by using electron microscopy, and we applied this method to the immunization of rabbits with an HIV-1 envelope glycoprotein vaccine candidate, BG505 SOSIP.664. We detected known epitopes within the polyclonal sera and revealed how antibody responses evolved during the prime-boosting strategy to ultimately result in a neutralizing antibody response. We uncovered previously unidentified epitopes, including an epitope proximal to one recognized by human broadly neutralizing antibodies as well as potentially distracting non-neutralizing epitopes. Our method provides an efficient and semiquantitative map of epitopes that are targeted in a polyclonal antibody response and should be of widespread utility in vaccine and infection studies.|
|External links||PubMed:30097292 / Publisher's page|
|Keywords||AIDS Vaccines / Animals / Antibodies, Neutralizing / Antibody Formation / Cell Line / Epitope Mapping / Epitopes / Female / HEK293 Cells / HIV Antibodies / HIV Infections / HIV-1 / Humans / Immunization / Immunoglobulin Fab Fragments / Microscopy, Electron / Rabbits / Recombinant Proteins / env Gene Products, Human Immunodeficiency Virus / IMMUNE SYSTEM / Fab / HIV / VIRAL PROTEIN / antibodies / antibody|
|Methods||EM (single particle) / X-ray diffraction|
|Resolution||1.795 - 20 A|
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