Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge /
PubMed Abstract
The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.
EMDB-23938, PDB-7mqa: Cryo-EM structure of the human SSU processome, state post-A1 Method: EM (single particle) / Resolution: 2.7 Å
EMDB-23939: Cryo-EM structure of the human SSU processome, state pre-A1 - raw maps Method: EM (single particle) / Resolution: 3.51 Å
EMDB-23940: Cryo-EM structure of the human SSU processome, state post-A1 - raw maps Method: EM (single particle) / Resolution: 2.65 Å
EMDB-24149: Cryo-EM structure of the human SSU processome, state pre-A1 - 5'ETS focused map Method: EM (single particle) / Resolution: 3.73 Å
EMDB-24150: Cryo-EM structure of the human SSU processome, state pre-A1 - Core focused map Method: EM (single particle) / Resolution: 3.29 Å
EMDB-24151: Cryo-EM structure of the human SSU processome, state pre-A1 - HEATR1 focused map Method: EM (single particle) / Resolution: 6.37 Å
EMDB-24152: Cryo-EM structure of the human SSU processome, state pre-A1 - NAT10-NOL10 focused map Method: EM (single particle) / Resolution: 3.67 Å
EMDB-24153: Cryo-EM structure of the human SSU processome, state pre-A1 - NEP1 focused map Method: EM (single particle) / Resolution: 3.54 Å
EMDB-24154: Cryo-EM structure of the human SSU processome, state pre-A1 - NGDN focused map Method: EM (single particle) / Resolution: 3.83 Å
EMDB-24155: Cryo-EM structure of the human SSU processome, state pre-A1 - PNO1 focused map Method: EM (single particle) / Resolution: 4.06 Å
EMDB-24156: Cryo-EM structure of the human SSU processome, state pre-A1 - TBL3 focused map Method: EM (single particle) / Resolution: 4.09 Å
EMDB-24157: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-CTD focused map Method: EM (single particle) / Resolution: 6.3 Å
EMDB-24158: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-NTD focused map Method: EM (single particle) / Resolution: 3.42 Å
EMDB-24159: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPA focused map Method: EM (single particle) / Resolution: 3.58 Å
EMDB-24160: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC focused map Method: EM (single particle) / Resolution: 4.45 Å
EMDB-24161: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC-Central focused map Method: EM (single particle) / Resolution: 3.73 Å
EMDB-24162: Cryo-EM structure of the human SSU processome, state post-A1 - Core focused map Method: EM (single particle) / Resolution: 2.63 Å
EMDB-24163: Cryo-EM structure of the human SSU processome, state post-A1 - DHX37 focused map Method: EM (single particle) / Resolution: 4.23 Å
EMDB-24164: Cryo-EM structure of the human SSU processome, state post-A1 - H44 focused map Method: EM (single particle) / Resolution: 3.87 Å
EMDB-24165: Cryo-EM structure of the human SSU processome, state post-A1 - HEATR1 focused map Method: EM (single particle) / Resolution: 3.2 Å
EMDB-24166: Cryo-EM structure of the human SSU processome, state post-A1 - probable MYBBP1A focused map Method: EM (single particle) / Resolution: 3.71 Å
EMDB-24167: Cryo-EM structure of the human SSU processome, state post-A1 - NOL11 focused map Method: EM (single particle) / Resolution: 3.57 Å
EMDB-24168: Cryo-EM structure of the human SSU processome, state post-A1 - RPS19-AROS focused map Method: EM (single particle) / Resolution: 2.99 Å
EMDB-24169: Cryo-EM structure of the human SSU processome, state post-A1 - UTP20 focused map Method: EM (single particle) / Resolution: 2.72 Å
EMDB-24170: Cryo-EM structure of the human SSU processome, state post-A1 - UTP6 focused map Method: EM (single particle) / Resolution: 2.96 Å
EMDB-24171: Cryo-EM structure of the human SSU processome, state post-A1 - UTPA focused map Method: EM (single particle) / Resolution: 2.78 Å
EMDB-24172: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC focused map Method: EM (single particle) / Resolution: 2.89 Å
EMDB-24173: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC-central focused map Method: EM (single particle) / Resolution: 2.6 Å
EMDB-24174: Cryo-EM structure of the human SSU processome - BYST-RPS12 focused map Method: EM (single particle) / Resolution: 3.46 Å
EMDB-24175: Cryo-EM structure of the human SSU processome - NOC4L-NOP14 focused map Method: EM (single particle) / Resolution: 3.41 Å
EMDB-24176: Cryo-EM structure of the human SSU processome - RRP12-BYST focused map Method: EM (single particle) / Resolution: 5.25 Å
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels
Nobel Prize for mechanically activated and temperature-gated ion channels
The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
EM Navigator can help to find cryo-EM structure data by both pioneers.
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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Keywords
homo sapiens (human)
saccharomyces cerevisiae (brewer's yeast)
