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Structure paper

TitleNucleolar maturation of the human small subunit processome.
Journal, issue, pagesScience, Vol. 373, Issue 6560, Page eabj5338, Year 2021
Publish dateSep 10, 2021
AuthorsSameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge /
PubMed AbstractThe human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.
External linksScience / PubMed:34516797 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.23 - 6.37 Å
Structure data

EMDB-23936, PDB-7mq8:
Cryo-EM structure of the human SSU processome, state pre-A1
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-23937, PDB-7mq9:
Cryo-EM structure of the human SSU processome, state pre-A1*
Method: EM (single particle) / Resolution: 3.87 Å

EMDB-23938, PDB-7mqa:
Cryo-EM structure of the human SSU processome, state post-A1
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-23939:
Cryo-EM structure of the human SSU processome, state pre-A1 - raw maps
Method: EM (single particle) / Resolution: 3.51 Å

EMDB-23940:
Cryo-EM structure of the human SSU processome, state post-A1 - raw maps
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-24149:
Cryo-EM structure of the human SSU processome, state pre-A1 - 5'ETS focused map
Method: EM (single particle) / Resolution: 3.73 Å

EMDB-24150:
Cryo-EM structure of the human SSU processome, state pre-A1 - Core focused map
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-24151:
Cryo-EM structure of the human SSU processome, state pre-A1 - HEATR1 focused map
Method: EM (single particle) / Resolution: 6.37 Å

EMDB-24152:
Cryo-EM structure of the human SSU processome, state pre-A1 - NAT10-NOL10 focused map
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-24153:
Cryo-EM structure of the human SSU processome, state pre-A1 - NEP1 focused map
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-24154:
Cryo-EM structure of the human SSU processome, state pre-A1 - NGDN focused map
Method: EM (single particle) / Resolution: 3.83 Å

EMDB-24155:
Cryo-EM structure of the human SSU processome, state pre-A1 - PNO1 focused map
Method: EM (single particle) / Resolution: 4.06 Å

EMDB-24156:
Cryo-EM structure of the human SSU processome, state pre-A1 - TBL3 focused map
Method: EM (single particle) / Resolution: 4.09 Å

EMDB-24157:
Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-CTD focused map
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-24158:
Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-NTD focused map
Method: EM (single particle) / Resolution: 3.42 Å

EMDB-24159:
Cryo-EM structure of the human SSU processome, state pre-A1 - UTPA focused map
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-24160:
Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC focused map
Method: EM (single particle) / Resolution: 4.45 Å

EMDB-24161:
Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC-Central focused map
Method: EM (single particle) / Resolution: 3.73 Å

EMDB-24162:
Cryo-EM structure of the human SSU processome, state post-A1 - Core focused map
Method: EM (single particle) / Resolution: 2.63 Å

EMDB-24163:
Cryo-EM structure of the human SSU processome, state post-A1 - DHX37 focused map
Method: EM (single particle) / Resolution: 4.23 Å

EMDB-24164:
Cryo-EM structure of the human SSU processome, state post-A1 - H44 focused map
Method: EM (single particle) / Resolution: 3.87 Å

EMDB-24165:
Cryo-EM structure of the human SSU processome, state post-A1 - HEATR1 focused map
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-24166:
Cryo-EM structure of the human SSU processome, state post-A1 - probable MYBBP1A focused map
Method: EM (single particle) / Resolution: 3.71 Å

EMDB-24167:
Cryo-EM structure of the human SSU processome, state post-A1 - NOL11 focused map
Method: EM (single particle) / Resolution: 3.57 Å

EMDB-24168:
Cryo-EM structure of the human SSU processome, state post-A1 - RPS19-AROS focused map
Method: EM (single particle) / Resolution: 2.99 Å

EMDB-24169:
Cryo-EM structure of the human SSU processome, state post-A1 - UTP20 focused map
Method: EM (single particle) / Resolution: 2.72 Å

EMDB-24170:
Cryo-EM structure of the human SSU processome, state post-A1 - UTP6 focused map
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-24171:
Cryo-EM structure of the human SSU processome, state post-A1 - UTPA focused map
Method: EM (single particle) / Resolution: 2.78 Å

EMDB-24172:
Cryo-EM structure of the human SSU processome, state post-A1 - UTPC focused map
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-24173:
Cryo-EM structure of the human SSU processome, state post-A1 - UTPC-central focused map
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-24174:
Cryo-EM structure of the human SSU processome - BYST-RPS12 focused map
Method: EM (single particle) / Resolution: 3.46 Å

EMDB-24175:
Cryo-EM structure of the human SSU processome - NOC4L-NOP14 focused map
Method: EM (single particle) / Resolution: 3.41 Å

EMDB-24176:
Cryo-EM structure of the human SSU processome - RRP12-BYST focused map
Method: EM (single particle) / Resolution: 5.25 Å

PDB-7mqj:
Dhr1 Helicase Core
Method: X-RAY DIFFRACTION / Resolution: 2.23 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • Human (human)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / Ribosomal assembly intermediate / HYDROLASE / Helicase / Ribosome Assembly

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