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Structure paper

TitleHuman CTP synthase filament structure reveals the active enzyme conformation.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 24, Issue 6, Page 507-514, Year 2017
Publish dateMay 1, 2017
AuthorsEric M Lynch / Derrick R Hicks / Matthew Shepherd / James A Endrizzi / Allison Maker / Jesse M Hansen / Rachael M Barry / Zemer Gitai / Enoch P Baldwin / Justin M Kollman
External linksPubMed:28459447 / Publisher's page
KeywordsCTP synthetase / Carbon-Nitrogen Ligases / Cryoelectron Microscopy / Crystallography, X-Ray / Humans / Macromolecular Substances / Models, Molecular / Protein Conformation / Protein Multimerization / LYASE / PYRIMIDINE BIOSYNTHESIS / ENZYME REGULATION VIA POLYMERIZATION / FEEDBACK INHIBITION / LIGASE / PROTEIN FIBRIL / nucleotide metabolism / enzyme / filament / active / metabolic filament
MethodsEM (single particle) / EM (helical sym.) / X-ray diffraction
Resolution2.7 - 17 A
Structure data

EMDB-8476:
human CTP synthase 1 - mutant H355A

EMDB-8490:
E. coli CTP synthase CC mutant filament

EMDB-8491:
E. coli CTP synthase CC mutant filament

EMDB-8504:
CryoEM structure of the CTP synthase filament at 4.6 Angstrom resolution

EMDB-8513:
E. coli CTP synthase CC mutant filament (product-bound)

EMDB-8474:
Cryo-EM structure of the human CTP synthase filament

EMDB-8475:
Cryo-EM structure of the E. coli CTP synthase tetramer

PDB-5tkv:
X-RAY CRYSTAL STRUCTURE OF THE "CLOSED" CONFORMATION OF CTP-INHIBITED E. COLI CYTIDINE TRIPHOSPHATE (CTP) SYNTHETASE

PDB-5u03:
Cryo-EM structure of the human CTP synthase filament

PDB-5u05:
Cryo-EM structure of the E. coli CTP synthase tetramer

PDB-5u3c:
CryoEM structure of the CTP synthase filament at 4.6 Angstrom resolution

PDB-5u6r:
E. coli CTP synthase CC mutant filament (product-bound)

Chemicals

ChemComp-GLN:
GLUTAMINEGlutamine

ChemComp-SO4:
SULFATE IONSulfate

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL2-Methyl-2,4-pentanediol

ChemComp-MG:
MAGNESIUM IONMagnesium

ChemComp-CTP:
CYTIDINE-5'-TRIPHOSPHATECytidine triphosphate

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL2-Methyl-2,4-pentanediol

ChemComp-HOH:
WATERWater

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATEAdenosine triphosphate

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATEUridine triphosphate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATEAdenosine diphosphate

SourceHomo sapiens / / human
Escherichia coli / / bacteria /
Escherichia coli (strain k12) / / bacteria /

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