Author results

1V04
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SERUM PARAOXONASE BY DIRECTED EVOLUTION
Descriptor:SERUM PARAOXONASE/ARYLESTERASE 1, CALCIUM ION, PHOSPHATE ION
Authors:Harel, M., Aharoni, A., Gaidukov, L., Brumshtein, B., Khersonsky, O., Yagur, S., Meged, R., Dvir, H., Ravelli, R.B.G., McCarthy, A., Toker, L., Silman, I., Sussman, J.L., Tawfik, D.S.
Deposit date:2004-03-22
Release date:2004-04-23
Last modified:2017-03-22
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Structure and Evolution of the Serum Paraoxonase Family of Detoxifying and Anti-Atherosclerotic Enzymes
Nat.Struct.Mol.Biol., 11, 2004
2BJM
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SPE7:ANTHRONE COMPLEX
Descriptor:IGE SPE7 HEAVY CHAIN, IGE SPE7 LIGHT CHAIN, ANTHRONE
Authors:James, L.C., Tawfik, D.S.
Deposit date:2005-02-04
Release date:2005-08-18
Last modified:2013-10-30
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Structure and Kinetics of a Transient Antibody Binding Intermediate Reveal a Kinetic Discrimination Mechanism in Antigen Recognition
Proc.Natl.Acad.Sci.USA, 102, 2005
3A3W
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STRUCTURE OF OPDA MUTANT (G60A/A80V/S92A/R118Q/K185R/Q206P/D208G/I260T/G273S) WITH DIETHYL 4-METHOXYPHENYL PHOSPHATE BOUND IN THE ACTIVE SITE
Descriptor:Phosphotriesterase, COBALT (II) ION, DIETHYL 4-METHOXYPHENYL PHOSPHATE
Authors:Ollis, D.L., Tawfik, D.S., Schenk, G., Jackson, C.J., Foo, J.L., Tokuriki, N., Afriat, L., Carr, P.D., Kim, H.K.
Deposit date:2009-06-23
Release date:2010-01-12
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase
Proc.Natl.Acad.Sci.USA, 2009
3A3X
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STRUCTURE OF OPDA MUTANT (G60A/A80V/R118Q/K185R/Q206P/D208G/I260T/G273S)
Descriptor:Phosphotriesterase, COBALT (II) ION
Authors:Ollis, D.L., Tawfik, D.S., Schenk, G., Jackson, C.J., Foo, J.L., Tokuriki, N., Afriat, L., Carr, P.D., Kim, H.K.
Deposit date:2009-06-23
Release date:2010-01-12
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase
Proc.Natl.Acad.Sci.USA, 2009
3GJN
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FOLLOWING EVOLUTIONARY PATHS TO HIGH AFFINITY AND SELECTIVITY PROTEIN-PROTEIN INTERACTIONS USING COLICIN7 AND IMMUNITY PROTEINS
Descriptor:Colicin-E9 immunity protein, Colicin-E7, ZINC ION
Authors:Dym, O., Tawfik, D.S.
Deposit date:2009-03-09
Release date:2009-09-15
Last modified:2013-03-27
Method:X-RAY DIFFRACTION (2.48 Å)
Cite:Following evolutionary paths to protein-protein interactions with high affinity and selectivity
Nat.Struct.Mol.Biol., 16, 2009
3GKL
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FOLLOWING EVOLUTIONARY PATHS TO HIGH AFFINITY AND SELECTIVITY PROTEIN-PROTEIN INTERACTIONS USING COLICIN7 AND IMMUNITY PROTEINS
Descriptor:Colicin-E9 immunity protein, Colicin-E7, ZINC ION
Authors:Dym, O., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2009-03-11
Release date:2009-09-08
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Following evolutionary paths to high affinity and selectivity protein-protein interactions
To be Published
3IIO
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EVOLUTIONARY OPTIMIZATION OF COMPUTATIONALLY DESIGNED ENZYMES: KEMP ELIMINASES OF THE KE07 SERIES
Descriptor:KE07
Authors:Khersonsky, O., Dym, O., Tawfik, D.S.
Deposit date:2009-08-03
Release date:2010-03-02
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series.
J.Mol.Biol., 396, 2010
3IIP
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EVOLUTIONARY OPTIMIZATION OF COMPUTATIONALLY DESIGNED ENZYMES: KEMP ELIMINASES OF THE KE07 SERIES
Descriptor:KE7 R6 3/7F, POLYETHYLENE GLYCOL (N=34)
Authors:Khersonsky, O., Dym, O., Tawfik, D.S.
Deposit date:2009-08-03
Release date:2010-03-02
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series.
J.Mol.Biol., 396, 2010
3IIV
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EVOLUTIONARY OPTIMIZATION OF COMPUTATIONALLY DESIGNED ENZYMES: KEMP ELIMINASES OF THE KE07 SERIES
Descriptor:KE7 KE7_R7_1/3H, MAGNESIUM ION
Authors:Khersonsky, O., Dym, O., Tawfik, D.S.
Deposit date:2009-08-03
Release date:2010-03-02
Last modified:2014-12-02
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series.
J.Mol.Biol., 396, 2010
3KIF
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THE CRYSTAL STRUCTURES OF TWO FRAGMENTS TRUNCATED FROM 5-BLADED BETA-PROPELLER LECTIN, TACHYLECTIN-2 (LIB1-B7-18 AND LIB2-D2-15)
Descriptor:5-bladed beta-propeller lectin, 2-ACETAMIDO-2-DEOXY-D-GLUCONO-1,5-LACTONE, SULFATE ION
Authors:Dym, O., Tawfik, D.S., Yadid, I., Israel Structural Proteomics Center (ISPC)
Deposit date:2009-11-02
Release date:2010-04-28
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Metamorphic proteins mediate evolutionary transitions of structure
Proc.Natl.Acad.Sci.USA, 107, 2010
3KIH
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THE CRYSTAL STRUCTURES OF TWO FRAGMENTS TRUNCATED FROM 5-BLADED BETA-PROPELLER LECTIN, TACHYLECTIN-2 (LIB2-D2-15)
Descriptor:5-bladed beta-propeller lectin, 2-ACETAMIDO-2-DEOXY-D-GLUCONO-1,5-LACTONE
Authors:Dym, O., Tawfik, D.S., Yadid, I., Israel Structural Proteomics Center (ISPC)
Deposit date:2009-11-02
Release date:2010-04-28
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.49 Å)
Cite:Metamorphic proteins mediate evolutionary transitions of structure
Proc.Natl.Acad.Sci.USA, 107, 2010
3NPU
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION
Descriptor:deoxyribose phosphate aldolase
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NPV
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION
Descriptor:deoxyribose phosphate aldolase
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (1.48 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NPW
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IN SILICO DESIGNED OF AN IMPROVED KEMP ELIMINASE KE70 MUTANT BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION
Descriptor:deoxyribose phosphate aldolase
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.14 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NPX
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION
Descriptor:deoxyribose phosphate aldolase
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (1.79 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NQ2
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION R2 3/5G
Descriptor:deoxyribose phosphate aldolase, IMIDAZOLE
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.02 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NQ8
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION R4 8/5A
Descriptor:deoxyribose phosphate aldolase, NITRATE ION, BENZAMIDINE
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (1.4 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NQV
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION R5 7/4A
Descriptor:deoxyribose phosphate aldolase, BENZAMIDINE
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-29
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3NR0
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION R6 6/10A
Descriptor:deoxyribose phosphate aldolase
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-06-30
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.19 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3Q2D
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OPTIMIZATION OF THE IN SILICO DESIGNED KEMP ELIMINASE KE70 BY COMPUTATIONAL DESIGN AND DIRECTED EVOLUTION
Descriptor:Deoxyribose phosphate aldolase, 5-nitro-1H-benzotriazole
Authors:Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Murphy, P., Dym, O., Albeck, S., Kiss, G., Houk, K.N., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2010-12-20
Release date:2011-02-09
Last modified:2014-03-19
Method:X-RAY DIFFRACTION (2.19 Å)
Cite:Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution
J.Mol.Biol., 407, 2011
3QVU
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CRYSTAL STRUCTURE OF ANCESTRAL VARIANT B9 OF SULT 1A1 IN COMPLEX WITH PAP AND P-NITROPHENOL
Descriptor:Sulfotransferase 1A1, P-NITROPHENOL, 1,2-ETHANEDIOL, ...
Authors:Alcolombri, U., Elias, M., Tawfik, D.S.
Deposit date:2011-02-26
Release date:2011-08-31
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Directed evolution of sulfotransferases and paraoxonases by ancestral libraries.
J.Mol.Biol., 411, 2011
3QVV
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CRYSTAL STRUCTURE OF ANCESTRAL VARIANT B9 OF SULT 1A1 IN COMPLEX WITH PAP AND 3-CYC
Descriptor:Sulfotransferase 1A1, ADENOSINE-3'-5'-DIPHOSPHATE, 7-hydroxy-2-oxo-2H-chromene-3-carbonitrile
Authors:Alkolombri, U., Elias, M., Tawfik, D.S.
Deposit date:2011-02-26
Release date:2011-08-31
Method:X-RAY DIFFRACTION (2.35 Å)
Cite:Directed evolution of sulfotransferases and paraoxonases by ancestral libraries.
J.Mol.Biol., 411, 2011
3SRE
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SERUM PARAOXONASE-1 BY DIRECTED EVOLUTION AT PH 6.5
Descriptor:serum paraoxonase, CALCIUM ION, PHOSPHATE ION, ...
Authors:Ben David, M., Elias, M., Silman, I., Sussman, J.L., Tawfik, D.S.
Deposit date:2011-07-07
Release date:2012-03-21
Last modified:2012-04-18
Method:X-RAY DIFFRACTION (1.99 Å)
Cite:Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1.
J.Mol.Biol., 418, 2012
3SRG
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SERUM PARAOXONASE-1 BY DIRECTED EVOLUTION AT PH 6.5 IN COMPLEX WITH 2-HYDROXYQUINOLINE
Descriptor:serum paraoxonase, CALCIUM ION, QUINOLIN-2(1H)-ONE, ...
Authors:Ben David, M., Elias, M., Silman, I., Sussman, J.L., Tawfik, D.S.
Deposit date:2011-07-07
Release date:2012-03-21
Last modified:2012-04-18
Method:X-RAY DIFFRACTION (2.19 Å)
Cite:Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1.
J.Mol.Biol., 418, 2012
3UXA
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DESIGNED PROTEIN KE59 R1 7/10H
Descriptor:Kemp eliminase KE59 R1 7/10H, PHOSPHATE ION
Authors:Khersonsky, O., Kiss, G., Roethlisberger, D., Dym, O., Albeck, S., Houk, K.N., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)
Deposit date:2011-12-05
Release date:2012-06-06
Last modified:2012-07-11
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.
Proc.Natl.Acad.Sci.USA, 109, 2012