8H6S
 
 | | Structure of acyltransferase VinK in complex with the loading acyl carrier protein of vicenistatin PKS | | Descriptor: | MAGNESIUM ION, Malonyl-CoA-[acyl-carrier-protein] transacylase, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, ... | | Authors: | Kawada, K, Miyanaga, A, Chisuga, T, Kudo, F, Eguchi, T. | | Deposit date: | 2022-10-18 | | Release date: | 2022-12-21 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | Structural Basis of Transient Interactions of Acyltransferase VinK with the Loading Acyl Carrier Protein of the Vicenistatin Modular Polyketide Synthase.
Biochemistry, 62, 2023
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8IN9
 | | The structure of the GfsA KSQ-AT didomain in complex with the GfsA ACP domain | | Descriptor: | N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, Polyketide synthase | | Authors: | Chisuga, T, Murakami, S, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2023-03-09 | | Release date: | 2023-05-31 | | Last modified: | 2023-06-28 | | Method: | X-RAY DIFFRACTION (3.4 Å) | | Cite: | Structure-Based Analysis of Transient Interactions between Ketosynthase-like Decarboxylase and Acyl Carrier Protein in a Loading Module of Modular Polyketide Synthase.
Acs Chem.Biol., 18, 2023
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8K4R
 | | Structure of VinM-VinL complex | | Descriptor: | Acyl-carrier-protein, Non-ribosomal peptide synthetase, SODIUM ION, ... | | Authors: | Miyanaga, A, Nagata, K, Nakajima, J, Chisuga, T, Kudo, F, Eguchi, T. | | Deposit date: | 2023-07-20 | | Release date: | 2023-11-01 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structural Basis of Amide-Forming Adenylation Enzyme VinM in Vicenistatin Biosynthesis.
Acs Chem.Biol., 18, 2023
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2ZTW
 | | Structure of 3-isopropylmalate dehydrogenase in complex with the inhibitor and NAD+ | | Descriptor: | (2Z)-2-hydroxy-3-(methylsulfanyl)prop-2-enoic acid, 3-isopropylmalate dehydrogenase, MAGNESIUM ION, ... | | Authors: | Nango, E, Kumasaka, T, Eguchi, T. | | Deposit date: | 2008-10-10 | | Release date: | 2009-10-13 | | Last modified: | 2023-11-01 | | Method: | X-RAY DIFFRACTION (2.79 Å) | | Cite: | Crystal structure of 3-isopropylmalate dehydrogenase in complex with NAD(+) and a designed inhibitor
Bioorg.Med.Chem., 17, 2009
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7VEE
 | | The ligand-free structure of GfsA KSQ-AT didomain | | Descriptor: | GLYCEROL, Polyketide synthase | | Authors: | Chisuga, T, Miyanaga, A, Nagai, A, Kudo, F, Eguchi, T. | | Deposit date: | 2021-09-08 | | Release date: | 2022-01-12 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (2.55 Å) | | Cite: | Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases.
Acs Chem.Biol., 17, 2022
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7VEF
 | | The structure of GfsA KSQ-AT didomain in complex with a malonate substrate analog | | Descriptor: | GLYCEROL, N-(2-acetamidoethyl)-2-nitro-ethanamide, Polyketide synthase | | Authors: | Chisuga, T, Miyanaga, A, Nagai, A, Kudo, F, Eguchi, T. | | Deposit date: | 2021-09-08 | | Release date: | 2022-01-12 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (2.65 Å) | | Cite: | Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases.
Acs Chem.Biol., 17, 2022
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5WSY
 | | The complex structure of SAV606 with N-carboxymethyl-3-aminobutyrate | | Descriptor: | (3~{R})-3-(2-hydroxy-2-oxoethylamino)butanoic acid, Uncharacterized protein | | Authors: | Chisuga, T, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2016-12-08 | | Release date: | 2017-05-31 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Structural analysis of the dual-function thioesterase SAV606 unravels the mechanism of Michael addition of glycine to an alpha , beta-unsaturated thioester.
J. Biol. Chem., 292, 2017
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5WSX
 | | The crystal structure of SAV606 | | Descriptor: | Uncharacterized protein | | Authors: | Chisuga, T, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2016-12-08 | | Release date: | 2017-05-31 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.4 Å) | | Cite: | Structural analysis of the dual-function thioesterase SAV606 unravels the mechanism of Michael addition of glycine to an alpha , beta-unsaturated thioester.
J. Biol. Chem., 292, 2017
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4ZM3
 | | Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type | | Descriptor: | Aminotransferase, DI(HYDROXYETHYL)ETHER, PYRIDOXAL-5'-PHOSPHATE, ... | | Authors: | Hirayama, A, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2015-05-02 | | Release date: | 2015-10-14 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.27 Å) | | Cite: | Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
Chembiochem, 16, 2015
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4ZM4
 | | Complex structure of PctV K276R mutant with PMP and 3-dehydroshkimate | | Descriptor: | (3E,4R,5R)-4,5-dihydroxy-3-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}cyclohex-1-ene-1-carboxylic acid, Aminotransferase, PYRIDOXAL-5'-PHOSPHATE | | Authors: | Hirayama, A, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2015-05-02 | | Release date: | 2015-10-14 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.4 Å) | | Cite: | Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
Chembiochem, 16, 2015
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5JJQ
 | | Crystal structure of IdnL1 | | Descriptor: | 5'-O-[(R)-{[(3S)-3-aminobutanoyl]oxy}(hydroxy)phosphoryl]adenosine, AMP-dependent synthetase and ligase, CHLORIDE ION | | Authors: | Cieslak, J, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2016-04-25 | | Release date: | 2017-03-22 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.6 Å) | | Cite: | Biochemical characterization and structural insight into aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6
Proteins, 85, 2017
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5JJP
 | | Crystal structure of CmiS6 | | Descriptor: | Nonribosomal peptide synthase | | Authors: | Cieslak, J, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2016-04-25 | | Release date: | 2017-03-22 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Biochemical characterization and structural insight into aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6
Proteins, 85, 2017
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7YKE
 | | Crystal structure of chondroitin ABC lyase I in complex with chondroitin disaccharide 4,6-sulfate | | Descriptor: | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-4,6-di-O-sulfo-beta-D-galactopyranose, Chondroitin sulfate ABC endolyase, MAGNESIUM ION | | Authors: | Takashima, M, Watanabe, I, Miyanaga, A, Eguchi, T. | | Deposit date: | 2022-07-22 | | Release date: | 2022-11-30 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (1.88 Å) | | Cite: | Biochemical and crystallographic assessments of the effect of 4,6-O-disulfated disaccharide moieties in chondroitin sulfate E on chondroitinase ABC I activity.
Febs J., 290, 2023
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2D2X
 | | Crystal structure of 2-deoxy-scyllo-inosose synthase | | Descriptor: | 2-deoxy-scyllo-inosose synthase, COBALT (II) ION, GLYCEROL, ... | | Authors: | Nango, E, Kumasaka, T, Tanaka, N, Kakinuma, K, Eguchi, T. | | Deposit date: | 2005-09-20 | | Release date: | 2006-10-03 | | Last modified: | 2011-07-13 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and NAD+
Proteins, 70, 2008
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7F2R
 | | Crystal structure of VinK-VinL covalent complex formed with a pantetheineamide cross-linking probe | | Descriptor: | Acyl-carrier-protein, Malonyl-CoA-[acyl-carrier-protein] transacylase, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide | | Authors: | Miyanaga, A, Ouchi, R, Kudo, F, Eguchi, T. | | Deposit date: | 2021-06-14 | | Release date: | 2021-09-01 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (1.95 Å) | | Cite: | Complex structure of the acyltransferase VinK and the carrier protein VinL with a pantetheine cross-linking probe.
Acta Crystallogr.,Sect.F, 77, 2021
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3ASJ
 | | Crystal structure of homoisocitrate dehydrogenase in complex with a designed inhibitor | | Descriptor: | (2Z)-3-[(carboxymethyl)sulfanyl]-2-hydroxyprop-2-enoic acid, (4S)-2-METHYL-2,4-PENTANEDIOL, 3-[(carboxymethyl)sulfanyl]-2-oxopropanoic acid, ... | | Authors: | Nango, E, Kumasaka, T, Eguchi, T. | | Deposit date: | 2010-12-13 | | Release date: | 2011-09-21 | | Last modified: | 2023-11-01 | | Method: | X-RAY DIFFRACTION (2.6 Å) | | Cite: | Structure of Thermus thermophilus homoisocitrate dehydrogenase in complex with a designed inhibitor
J.Biochem., 150, 2011
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5Y1I
 | | The crystal structure of GfsF | | Descriptor: | Cytochrome P450, DI(HYDROXYETHYL)ETHER, PROTOPORPHYRIN IX CONTAINING FE, ... | | Authors: | Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2017-07-20 | | Release date: | 2017-09-13 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Substrate Recognition by a Dual-Function P450 Monooxygenase GfsF Involved in FD-891 Biosynthesis
Chembiochem, 18, 2017
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5ZDM
 | | The ligand-free structure of FomD | | Descriptor: | CALCIUM ION, FomD, GLYCEROL | | Authors: | Sato, S, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2018-02-23 | | Release date: | 2018-07-25 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (1.38 Å) | | Cite: | Biochemical and Structural Analysis of FomD That Catalyzes the Hydrolysis of Cytidylyl ( S)-2-Hydroxypropylphosphonate in Fosfomycin Biosynthesis.
Biochemistry, 57, 2018
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5ZDN
 | | The complex structure of FomD with CDP | | Descriptor: | CYTIDINE-5'-DIPHOSPHATE, FomD, GLYCEROL, ... | | Authors: | Sato, S, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2018-02-23 | | Release date: | 2018-07-25 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (2.02 Å) | | Cite: | Biochemical and Structural Analysis of FomD That Catalyzes the Hydrolysis of Cytidylyl ( S)-2-Hydroxypropylphosphonate in Fosfomycin Biosynthesis.
Biochemistry, 57, 2018
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3WAD
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3WAG
 | | Crystal structure of glycosyltransferase VinC in complex with DTDP | | Descriptor: | Glycosyltransferase, MAGNESIUM ION, PHOSPHATE ION, ... | | Authors: | Nango, E, Minami, A, Kumasaka, T, Eguchi, T. | | Deposit date: | 2013-05-02 | | Release date: | 2014-06-04 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Crystal Structure of Glycosyltransferase VinC Involved in the Biosynthesis of Vicenistatin
To be Published
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5CZD
 | | The complex structure of VinK with VinL | | Descriptor: | 1,1'-ethane-1,2-diyldipyrrolidine-2,5-dione, 4'-PHOSPHOPANTETHEINE, Acyl-carrier-protein, ... | | Authors: | Miyanaga, A, Iwasawa, S, Shinohara, Y, Kudo, F, Eguchi, T. | | Deposit date: | 2015-07-31 | | Release date: | 2016-02-03 | | Last modified: | 2023-11-08 | | Method: | X-RAY DIFFRACTION (2.34 Å) | | Cite: | Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis.
Proc.Natl.Acad.Sci.USA, 113, 2016
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5CZC
 | | The structure of VinK | | Descriptor: | CALCIUM ION, GLYCEROL, Malonyl-CoA-[acyl-carrier-protein] transacylase | | Authors: | Miyanaga, A, Iwasawa, S, Shinohara, Y, Kudo, F, Eguchi, T. | | Deposit date: | 2015-07-31 | | Release date: | 2016-02-03 | | Last modified: | 2024-03-20 | | Method: | X-RAY DIFFRACTION (1.8 Å) | | Cite: | Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis.
Proc.Natl.Acad.Sci.USA, 113, 2016
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6J38
 | | Crystal structure of CmiS2 | | Descriptor: | FAD-dependent glycine oxydase, FLAVIN-ADENINE DINUCLEOTIDE | | Authors: | Kawasaki, D, Chisuga, T, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2019-01-04 | | Release date: | 2019-06-12 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structural Analysis of the Glycine Oxidase Homologue CmiS2 Reveals a Unique Substrate Recognition Mechanism for Formation of a beta-Amino Acid Starter Unit in Cremimycin Biosynthesis.
Biochemistry, 58, 2019
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6J39
 | | Crystal structure of CmiS2 with inhibitor | | Descriptor: | (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid, FAD-dependent glycine oxydase, FLAVIN-ADENINE DINUCLEOTIDE | | Authors: | Kawasaki, D, Chisuga, T, Miyanaga, A, Kudo, F, Eguchi, T. | | Deposit date: | 2019-01-04 | | Release date: | 2019-06-12 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (2.45 Å) | | Cite: | Structural Analysis of the Glycine Oxidase Homologue CmiS2 Reveals a Unique Substrate Recognition Mechanism for Formation of a beta-Amino Acid Starter Unit in Cremimycin Biosynthesis.
Biochemistry, 58, 2019
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