4ZM3
Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type
Summary for 4ZM3
| Entry DOI | 10.2210/pdb4zm3/pdb |
| Related | 4ZM4 |
| Descriptor | Aminotransferase, PYRIDOXAL-5'-PHOSPHATE, TRIETHYLENE GLYCOL, ... (5 entities in total) |
| Functional Keywords | pyridoxal 5-phosphate, aminotransferase, transferase |
| Biological source | Streptomyces pactum |
| Total number of polymer chains | 4 |
| Total formula weight | 200239.17 |
| Authors | Hirayama, A.,Miyanaga, A.,Kudo, F.,Eguchi, T. (deposition date: 2015-05-02, release date: 2015-10-14, Last modification date: 2023-11-08) |
| Primary citation | Hirayama, A.,Miyanaga, A.,Kudo, F.,Eguchi, T. Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin. Chembiochem, 16:2484-2490, 2015 Cited by PubMed Abstract: Mutational analysis of the pyridoxal 5'-phosphate (PLP)-dependent enzyme PctV was carried out to elucidate the multi-step reaction mechanism for the formation of 3-aminobenzoate (3-ABA) from 3-dehydroshikimate (3-DSA). Introduction of mutation K276R led to the accumulation of a quinonoid intermediate with an absorption maximum at 580 nm after the reaction of pyridoxamine 5'-phosphate (PMP) with 3-DSA. The chemical structure of this intermediate was supported by X-ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3-ABA. They also indicate that Lys276 (in the active site of PctV) plays multiple roles, including acid/base catalysis during the dehydration reaction of the quinonoid intermediate. PubMed: 26426567DOI: 10.1002/cbic.201500426 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.27 Å) |
Structure validation
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