8DYE

Crystal structure of human SDHA-SDHAF4 assembly intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial	polymer	611	67150.9	1	UniProt (P31040) Pfam (PF00890) Pfam (PF02910) In PDB	Homo sapiens (human)	Flavoprotein subunit of complex II,Fp
2	B	Succinate dehydrogenase assembly factor 4, mitochondrial	polymer	98	11227.4	1	UniProt (Q5VUM1) Pfam (PF07896) In PDB	Homo sapiens (human)	SDH assembly factor 4,SDHAF4
3	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
4	A	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	16	Chemie (EDO)
6	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8		water	water		18.0	422	Chemie (HOH)

Sequence modifications

B: 34 - 108 (UniProt: Q5VUM1)

PDB	External Database	Details
Met 11	-	initiating methionine
Arg 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Glu 20	-	expression tag
Asn 21	-	expression tag
Leu 22	-	expression tag
Tyr 23	-	expression tag
Phe 24	-	expression tag
Gln 25	-	expression tag
Gly 26	-	expression tag
Ile 27	-	expression tag
Asp 28	-	expression tag
Pro 29	-	expression tag
Phe 30	-	expression tag
Thr 31	-	expression tag
Gly 32	-	expression tag
Ser 33	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78378.4
Non-Polymers*	Number of molecules	23
	Total formula weight	2202.9
All*	Total formula weight	80581.3

*Water molecules are not included.