8DYE
Crystal structure of human SDHA-SDHAF4 assembly intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-06-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 139.049, 69.299, 72.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.570 - 1.440 |
R-factor | 0.1559 |
Rwork | 0.155 |
R-free | 0.17280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vax 3sfd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.977 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.440 | 1.450 |
Rmerge | 0.090 | 1.390 |
Number of reflections | 125714 | 6190 |
<I/σ(I)> | 35.61 | 1.53 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7 | |
CC(1/2) | 0.993 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | 100 mM Sodium malonate, 12 % w/v PEG 3350, 1 mM DTT |