8CTZ

12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Ribonuclease H	polymer	142	16329.5	3	UniProt (Q9KEI9) In PDB	Alkalihalobacillus halodurans	RNase H
2	D, E	DNA (5'-D(*CP*GP*CP*(6OG)P*AP*AP*TP*TP*(OWR)P*GP*CP*G)-3')	polymer	12	3734.5	2		synthetic construct
3	A, B, C	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	B, C	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	C	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	C, D	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
8		water	water		18.0	119	Chemie (HOH)

Sequence modifications

A, B, C: 59 - 196 (UniProt: Q9KEI9)

PDB	External Database	Details
Gly 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Met 58	-	expression tag
Asn 132	Asp 132	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	56457.5
Non-Polymers*	Number of molecules	12
	Total formula weight	773.9
All*	Total formula weight	57231.3

*Water molecules are not included.