7T9I

Native human TSH bound to human Thyrotropin receptor in complex with miniGs399 (composite structure)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycoprotein hormones alpha chain	polymer	92	10217.8	1	UniProt (P01215) Pfam (PF00236) In PDB	Homo sapiens (human)	Anterior pituitary glycoprotein hormones common subunit alpha,Choriogonadotropin alpha chain,Chorionic gonadotrophin subunit alpha,CG-alpha,Follicle-stimulating hormone alpha chain,FSH-alpha,Follitropin alpha chain,Luteinizing hormone alpha chain,LSH-alpha,Lutropin alpha chain,Thyroid-stimulating hormone alpha chain,TSH-alpha,Thyrotropin alpha chain
2	B	Thyrotropin subunit beta	polymer	112	12916.0	1	UniProt (P01222) Pfam (PF00007) In PDB	Homo sapiens (human)	Thyroid-stimulating hormone subunit beta,TSH-B,TSH-beta,Thyrotropin beta chain
3	N	Nanobody 35	polymer	142	15398.1	1		Lama glama
4	R	Thyrotropin receptor	polymer	702	79742.4	1	UniProt (A0A0A0MTJ0) Pfam (PF13306) Pfam (PF00001) In PDB	Homo sapiens (human)
5	X	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30137.0	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
6	Y	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	370	40786.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
7	Z	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
8	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
9	A, B, R	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

R: 22 - 764 (UniProt: A0A0A0MTJ0)

PDB	External Database	Details
Asp 13	-	expression tag
Tyr 14	-	expression tag
Lys 15	-	expression tag
Asp 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Gly 20	-	expression tag
Thr 21	-	expression tag
-	Ala 317	deletion
-	Leu 318	deletion
-	Asn 319	deletion
-	Ser 320	deletion
-	Pro 321	deletion
-	Leu 322	deletion
-	His 323	deletion
-	Gln 324	deletion
-	Glu 325	deletion
-	Tyr 326	deletion
-	Glu 327	deletion
-	Glu 328	deletion
-	Asn 329	deletion
-	Leu 330	deletion
-	Gly 331	deletion
-	Asp 332	deletion
-	Ser 333	deletion
-	Ile 334	deletion
-	Val 335	deletion
-	Gly 336	deletion
-	Tyr 337	deletion
-	Lys 338	deletion
-	Glu 339	deletion
-	Lys 340	deletion
-	Ser 341	deletion
-	Lys 342	deletion
-	Phe 343	deletion
-	Gln 344	deletion
-	Asp 345	deletion
-	Thr 346	deletion
-	His 347	deletion
-	Asn 348	deletion
-	Asn 349	deletion
-	Ala 350	deletion
-	His 351	deletion
-	Tyr 352	deletion
-	Tyr 353	deletion
-	Val 354	deletion
-	Phe 355	deletion
-	Phe 356	deletion
-	Glu 357	deletion
-	Glu 358	deletion
-	Gln 359	deletion
-	Glu 360	deletion
-	Asp 361	deletion
-	Glu 362	deletion
-	Ile 363	deletion
-	Ile 364	deletion
-	Gly 365	deletion
-	Phe 366	deletion

X: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Leu -4	-	expression tag
Glu -3	-	expression tag
Val -2	-	expression tag
Leu -1	-	expression tag
Phe 0	-	expression tag
Gln 1	-	expression tag
Gly 2	-	expression tag
Pro 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Asn 6	-	expression tag
Ser 7	-	expression tag
Lys 8	-	expression tag
Thr 9	-	expression tag
Glu 10	-	expression tag
Asp 11	-	expression tag
Gln 12	-	expression tag
Arg 13	-	expression tag
Asn 14	-	expression tag
Glu 15	-	expression tag
Glu 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Gln 19	-	expression tag
Arg 20	-	expression tag
Glu 21	-	expression tag
Ala 22	-	expression tag
Asn 23	-	expression tag
Lys 24	-	expression tag
Lys 25	-	expression tag
Ile 26	-	expression tag
Glu 27	-	expression tag
Lys 28	-	expression tag
Gln 29	-	expression tag
Leu 30	-	expression tag
Gln 31	-	expression tag
Lys 32	-	expression tag
Asp 33	-	expression tag
Lys 34	-	expression tag
Gln 35	-	expression tag
Val 36	-	expression tag
Tyr 37	-	expression tag
Arg 38	-	expression tag
Ala 39	-	expression tag
Thr 40	-	expression tag
His 41	-	expression tag
Arg 42	-	expression tag
Leu 43	-	expression tag
Leu 44	-	expression tag
Leu 45	-	expression tag
Leu 46	-	expression tag
Gly 47	-	expression tag
Ala 48	-	expression tag
Asp 49	-	expression tag
Asn 50	-	expression tag
Ser 51	-	expression tag
Gly 52	-	expression tag
Lys 53	-	expression tag
Ser 54	-	expression tag
Thr 55	-	expression tag
Ile 56	-	expression tag
Val 57	-	expression tag
Lys 58	-	expression tag
Gln 59	-	expression tag
Met 60	-	expression tag
Arg 61	-	expression tag
Ile 193	-	expression tag
Leu 194	-	expression tag
His 195	-	expression tag
Gly 196	-	expression tag
Gly 197	-	expression tag
Ser 198	-	expression tag
Gly 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Gly 202	-	expression tag
Gly 203	-	expression tag
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Leu 308	Ile 308	conflict
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict

Y: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -29	-	initiating methionine
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Leu -22	-	expression tag
Glu -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Gln -17	-	expression tag
Gly -16	-	expression tag
Pro -15	-	expression tag
Glu -14	-	expression tag
Asp -13	-	expression tag
Gln -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Leu -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	197058.9
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	198810.6

*Water molecules are not included.