4WQ5

YgjD(V85E)-YeaZ heterodimer in complex with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	tRNA N6-adenosine threonylcarbamoyltransferase	polymer	343	36909.2	2	UniProt (P05852) Pfam (PF00814) In PDB	Escherichia coli	N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD,tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
2	C, D	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB	polymer	237	26033.6	2	UniProt (P76256) Pfam (PF00814) In PDB	Escherichia coli	t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
5	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
6	A, B, C, D	ACETATE ION	non-polymer		59.0	8	Chemie (ACT)
7	C	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8		water	water		18.0	115	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: P05852)

PDB	External Database	Details
Glu 85	Val 85	engineered mutation
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag

C, D: 1 - 231 (UniProt: P76256)

PDB	External Database	Details
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	125885.7
Non-Polymers*	Number of molecules	15
	Total formula weight	1739.1
All*	Total formula weight	127624.8

*Water molecules are not included.