4WQ5
YgjD(V85E)-YeaZ heterodimer in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2012-12-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 |
Unit cell lengths | 63.690, 68.080, 87.340 |
Unit cell angles | 109.31, 92.56, 117.94 |
Refinement procedure
Resolution | 42.620 - 2.330 |
R-factor | 0.1825 |
Rwork | 0.180 |
R-free | 0.23323 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.313 |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.330 |
Number of reflections | 48866 |
<I/σ(I)> | 13.06 |
Completeness [%] | 96.0 |
Redundancy | 3.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH 7.5, 26% PEG4000, 0.3 M Lithium Sulfate and 0.1 Sodium Acetate |