4MEX

Crystal structure of Escherichia coli RNA polymerase in complex with salinamide A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	DNA-directed RNA polymerase subunit alpha	polymer	335	37387.5	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	2	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	F, L	RNA polymerase sigma factor RpoD	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli	Sigma-70
6	M, N	Salinamide A	polymer	9	1056.2	2	BIRD (PRD_001162)	Streptomyces sp.
7	D, J	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	D, J	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, B, G, H: -5 - 329 (UniProt: P0A7Z4)

PDB	External Database	Details
His -4	-	EXPRESSION TAG
His -3	-	EXPRESSION TAG
His -2	-	EXPRESSION TAG
His -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
His 1	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	925241.4
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	925551.7

*Water molecules are not included.