4MEX
Crystal structure of Escherichia coli RNA polymerase in complex with salinamide A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 185.800, 208.188, 308.179 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.868 - 3.902 |
R-factor | 0.2867 |
Rwork | 0.286 |
R-free | 0.32500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((AutoMR)) |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.970 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.118 | 0.891 |
Number of reflections | 108818 | |
<I/σ(I)> | 15.455 | 1.547 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1M HEPES, 0.2M calcium chloride, 18% PEG400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |