4IZ5

Structure of the complex between ERK2 phosphomimetic mutant and PEA-15

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Mitogen-activated protein kinase 1	polymer	356	41126.2	4	UniProt (P28482) Pfam (PF00069) In PDB	Homo sapiens (human)	MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2
2	E, F, G, H	Astrocytic phosphoprotein PEA-15	polymer	133	15343.4	4	UniProt (Q15121) Pfam (PF01335) In PDB	Homo sapiens (human)	15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PED
3	A, B, C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4	D, A, B, C	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)

Sequence modifications

A, B, C, D: 8 - 360 (UniProt: P28482)

PDB	External Database	Details
Gly 5	-	expression tag
Pro 6	-	expression tag
Gly 7	-	expression tag
Glu 185	Thr 185	engineered mutation

E, F, G, H: 1 - 130 (UniProt: Q15121)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	225878.4
Non-Polymers*	Number of molecules	20
	Total formula weight	3245.8
All*	Total formula weight	229124.2

*Water molecules are not included.