4E45

Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, F, H, K...	Centromere protein S	polymer	112	13042.7	6	UniProt (Q8N2Z9) Pfam (PF15630) In PDB	Homo sapiens (human)	CENP-S, Apoptosis-inducing TAF9-like domain-containing protein 1, FANCM-interacting histone fold protein 1, Fanconi anemia-associated polypeptide of 16 kDa
2	B, D, G, I, L...	Centromere protein X	polymer	83	9116.5	6	UniProt (A8MT69) Pfam (PF09415) In PDB	Homo sapiens (human)	CENP-X, FANCM-interacting histone fold protein 2, Fanconi anemia-associated polypeptide of 10 kDa, Retinoic acid-inducible gene D9 protein homolog, Stimulated by retinoic acid gene 13 protein homolog
3	E, J, O	Fanconi anemia group M protein	polymer	137	16333.2	3	UniProt (Q8IYD8) Pfam (PF16783) In PDB	Homo sapiens (human)	Protein FACM, ATP-dependent RNA helicase FANCM, Fanconi anemia-associated polypeptide of 250 kDa, FAAP250, Protein Hef ortholog
4	B, H	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	D, J, N	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
6		water	water		18.0	546	Chemie (HOH)

Sequence modifications

A, C, F, H, K, M: 1 - 110 (UniProt: Q8N2Z9)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

B, D, G, I, L, N: 1 - 81 (UniProt: A8MT69)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

E, J, O: 667 - 800 (UniProt: Q8IYD8)

PDB	External Database	Details
Gly 664	-	expression tag
Ala 665	-	expression tag
Met 666	-	expression tag
Pro 668	Gly 668	conflict

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	181955.0
Non-Polymers*	Number of molecules	5
	Total formula weight	380.4
All*	Total formula weight	182335.4

*Water molecules are not included.