4E45

Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033180
Spacegroup name	P 1 21 1
Unit cell lengths	110.980, 69.970, 116.050
Unit cell angles	90.00, 91.60, 90.00

Refinement procedure

Resolution	49.510 - 2.000
R-factor	0.204
Rwork	0.202
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1taf
RMSD bond length	0.015
RMSD bond angle	1.520
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.510		2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.052	0.033	0.512
Number of reflections	119106	872	8864
<I/σ(I)>	14.9	29.3	3
Completeness [%]	98.9	60.3	99.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	298	Tracking Code C4, 0.1M Tris pH 7.8, 0.2M LiCl, 0.1M Na2SO4, 17.5% w/v PEG3350, 10% glycerol cryo., vapor diffusion, sitting drop, temperature 298K