Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7EWR

Cryo-EM structure of human GPR158 in complex with RGS7-Gbeta5 in a 2:2:2 ratio

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004930	molecular_function	G protein-coupled receptor activity
A	0006091	biological_process	generation of precursor metabolites and energy
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0008218	biological_process	bioluminescence
A	0016020	cellular_component	membrane
B	0004930	molecular_function	G protein-coupled receptor activity
B	0006091	biological_process	generation of precursor metabolites and energy
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0008218	biological_process	bioluminescence
B	0016020	cellular_component	membrane
C	0001965	molecular_function	G-protein alpha-subunit binding
C	0001975	biological_process	response to amphetamine
C	0003924	molecular_function	GTPase activity
C	0005096	molecular_function	GTPase activator activity
C	0005635	cellular_component	nuclear envelope
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway
C	0009968	biological_process	negative regulation of signal transduction
C	0031681	molecular_function	G-protein beta-subunit binding
C	0032991	cellular_component	protein-containing complex
C	0035556	biological_process	intracellular signal transduction
C	0043005	cellular_component	neuron projection
C	0043547	biological_process	positive regulation of GTPase activity
C	0045471	biological_process	response to ethanol
C	0045744	biological_process	negative regulation of G protein-coupled receptor signaling pathway
C	1901381	biological_process	positive regulation of potassium ion transmembrane transport
D	0003924	molecular_function	GTPase activity
D	0005096	molecular_function	GTPase activator activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005834	cellular_component	heterotrimeric G-protein complex
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007212	biological_process	dopamine receptor signaling pathway
D	0016020	cellular_component	membrane
D	0030159	molecular_function	signaling receptor complex adaptor activity
D	0031682	molecular_function	G-protein gamma-subunit binding
D	0036367	biological_process	light adaption
D	0043547	biological_process	positive regulation of GTPase activity
D	0051087	molecular_function	protein-folding chaperone binding
D	0098793	cellular_component	presynapse
D	1901386	biological_process	negative regulation of voltage-gated calcium channel activity
D	1902773	cellular_component	GTPase activator complex
D	1990603	biological_process	dark adaptation
E	0001965	molecular_function	G-protein alpha-subunit binding
E	0001975	biological_process	response to amphetamine
E	0003924	molecular_function	GTPase activity
E	0005096	molecular_function	GTPase activator activity
E	0005635	cellular_component	nuclear envelope
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway
E	0009968	biological_process	negative regulation of signal transduction
E	0031681	molecular_function	G-protein beta-subunit binding
E	0032991	cellular_component	protein-containing complex
E	0035556	biological_process	intracellular signal transduction
E	0043005	cellular_component	neuron projection
E	0043547	biological_process	positive regulation of GTPase activity
E	0045471	biological_process	response to ethanol
E	0045744	biological_process	negative regulation of G protein-coupled receptor signaling pathway
E	1901381	biological_process	positive regulation of potassium ion transmembrane transport
F	0003924	molecular_function	GTPase activity
F	0005096	molecular_function	GTPase activator activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005834	cellular_component	heterotrimeric G-protein complex
F	0005886	cellular_component	plasma membrane
F	0007165	biological_process	signal transduction
F	0007186	biological_process	G protein-coupled receptor signaling pathway
F	0007212	biological_process	dopamine receptor signaling pathway
F	0016020	cellular_component	membrane
F	0030159	molecular_function	signaling receptor complex adaptor activity
F	0031682	molecular_function	G-protein gamma-subunit binding
F	0036367	biological_process	light adaption
F	0043547	biological_process	positive regulation of GTPase activity
F	0051087	molecular_function	protein-folding chaperone binding
F	0098793	cellular_component	presynapse
F	1901386	biological_process	negative regulation of voltage-gated calcium channel activity
F	1902773	cellular_component	GTPase activator complex
F	1990603	biological_process	dark adaptation

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. IVSSsqDgKVIVWDS

Chain	Residue	Details
F	ILE78-SER92
F	ILE168-VAL182
F	LEU298-VAL312

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	858
Details	TOPO_DOM: Extracellular => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	ALA24-ARG417
A	VAL548-ASP576
A	LEU634-TRP642
B	ALA24-ARG417
B	VAL548-ASP576
B	LEU634-TRP642

site_id	SWS_FT_FI2
Number of Residues	42
Details	TRANSMEM: Helical; Name=1 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	LEU418-TYR439
B	LEU418-TYR439

site_id	SWS_FT_FI3
Number of Residues	94
Details	TOPO_DOM: Cytoplasmic => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	HIS440-GLY451
A	LYS502-ARG525
A	TYR598-ARG611
B	HIS440-GLY451
B	LYS502-ARG525
B	TYR598-ARG611

site_id	SWS_FT_FI4
Number of Residues	44
Details	TRANSMEM: Helical; Name=2 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	LEU452-PHE474
B	LEU452-PHE474

site_id	SWS_FT_FI5
Number of Residues	6
Details	TOPO_DOM: Extracellular => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0000305, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	GLU475-THR478
B	GLU475-THR478

site_id	SWS_FT_FI6
Number of Residues	44
Details	TRANSMEM: Helical; Name=3 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	PHE479-LEU501
B	PHE479-LEU501

site_id	SWS_FT_FI7
Number of Residues	42
Details	TRANSMEM: Helical; Name=4 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	VAL526-SER547
B	VAL526-SER547

site_id	SWS_FT_FI8
Number of Residues	40
Details	TRANSMEM: Helical; Name=5 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	ARG577-CYS597
B	ARG577-CYS597

site_id	SWS_FT_FI9
Number of Residues	42
Details	TRANSMEM: Helical; Name=6 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	TYR612-VAL633
B	TYR612-VAL633

site_id	SWS_FT_FI10
Number of Residues	42
Details	TRANSMEM: Helical; Name=7 => ECO:0000269\|PubMed:34793198, ECO:0000269\|PubMed:34815401, ECO:0007744\|PDB:7EWL, ECO:0007744\|PDB:7EWP, ECO:0007744\|PDB:7EWR, ECO:0007744\|PDB:7SHE, ECO:0007744\|PDB:7SHF

Chain	Residue	Details
A	MET643-ILE664
B	MET643-ILE664

site_id	SWS_FT_FI11
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305\|PubMed:36996198

Chain	Residue	Details
B	ARG173
B	GLU271
B	ASP307
A	SER172
A	ARG173
A	GLU271
A	ASP307
B	SER172

site_id	SWS_FT_FI12
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q8C419

Chain	Residue	Details
A	SER705
B	SER694
B	SER705
B	SER708
A	SER708
A	SER694

site_id	SWS_FT_FI13
Number of Residues	10
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN98
A	ASN143
A	ASN215
A	ASN274
A	ASN333
B	ASN98
B	ASN143
B	ASN215
B	ASN274
B	ASN333

site_id	SWS_FT_FI14
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:18655026

Chain	Residue	Details
A	LYS774
B	LYS774

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)