6QFA
CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004888 | molecular_function | transmembrane signaling receptor activity |
| A | 0004890 | molecular_function | GABA-A receptor activity |
| A | 0005216 | molecular_function | monoatomic ion channel activity |
| A | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0016020 | cellular_component | membrane |
| A | 0034220 | biological_process | monoatomic ion transmembrane transport |
| B | 0004888 | molecular_function | transmembrane signaling receptor activity |
| B | 0004890 | molecular_function | GABA-A receptor activity |
| B | 0005216 | molecular_function | monoatomic ion channel activity |
| B | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0016020 | cellular_component | membrane |
| B | 0034220 | biological_process | monoatomic ion transmembrane transport |
| C | 0004888 | molecular_function | transmembrane signaling receptor activity |
| C | 0004890 | molecular_function | GABA-A receptor activity |
| C | 0005216 | molecular_function | monoatomic ion channel activity |
| C | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| C | 0006811 | biological_process | monoatomic ion transport |
| C | 0016020 | cellular_component | membrane |
| C | 0034220 | biological_process | monoatomic ion transmembrane transport |
| D | 0004888 | molecular_function | transmembrane signaling receptor activity |
| D | 0004890 | molecular_function | GABA-A receptor activity |
| D | 0005216 | molecular_function | monoatomic ion channel activity |
| D | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| D | 0006811 | biological_process | monoatomic ion transport |
| D | 0016020 | cellular_component | membrane |
| D | 0034220 | biological_process | monoatomic ion transmembrane transport |
| E | 0004888 | molecular_function | transmembrane signaling receptor activity |
| E | 0004890 | molecular_function | GABA-A receptor activity |
| E | 0005216 | molecular_function | monoatomic ion channel activity |
| E | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| E | 0006811 | biological_process | monoatomic ion transport |
| E | 0016020 | cellular_component | membrane |
| E | 0034220 | biological_process | monoatomic ion transmembrane transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | binding site for residue HSM B 502 |
| Chain | Residue |
| A | ASP43 |
| A | TYR62 |
| B | TYR97 |
| B | GLU155 |
| B | SER156 |
| B | TYR157 |
| B | PHE200 |
| B | TYR205 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue HSM C 502 |
| Chain | Residue |
| B | TYR62 |
| C | TYR97 |
| C | GLU155 |
| C | SER156 |
| C | TYR157 |
| C | PHE200 |
| C | TYR205 |
| B | ASP43 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | binding site for residue HSM D 502 |
| Chain | Residue |
| C | ASP43 |
| C | TYR62 |
| D | TYR97 |
| D | GLU155 |
| D | SER156 |
| D | TYR157 |
| D | PHE200 |
| D | TYR205 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue HSM E 502 |
| Chain | Residue |
| D | ASP43 |
| D | TYR62 |
| E | TYR97 |
| E | GLU155 |
| E | SER156 |
| E | TYR157 |
| E | PHE200 |
| E | TYR205 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue HSM A 502 |
| Chain | Residue |
| A | TYR97 |
| A | GLU155 |
| A | SER156 |
| A | TYR157 |
| A | PHE200 |
| A | TYR205 |
| E | ASP43 |
| E | TYR62 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for Mono-Saccharide NAG A 501 bound to ASN A 80 |
| Chain | Residue |
| A | PRO78 |
| A | ASN80 |
| A | HIS119 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | binding site for Poly-Saccharide residues NAG A 502 through MAN A 506 bound to ASN A 149 |
| Chain | Residue |
| A | ASN149 |
| A | ARG192 |
| A | SER195 |
| A | ARG196 |
| A | ASN197 |
| A | SER211 |
| A | ARG213 |
| O | ASN417 |
| O | TYR418 |
| O | ASP500 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for Mono-Saccharide NAG B 501 bound to ASN B 80 |
| Chain | Residue |
| B | PRO78 |
| B | ASN80 |
| B | HIS119 |
| site_id | AC9 |
| Number of Residues | 10 |
| Details | binding site for Poly-Saccharide residues NAG B 502 through MAN B 506 bound to ASN B 149 |
| Chain | Residue |
| B | ASN149 |
| B | ARG192 |
| B | SER195 |
| B | ARG196 |
| B | ASN197 |
| B | SER211 |
| B | ARG213 |
| K | ASN417 |
| K | TYR418 |
| K | ASP500 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for Mono-Saccharide NAG C 501 bound to ASN C 80 |
| Chain | Residue |
| C | PRO78 |
| C | ASN80 |
| C | HIS119 |
| site_id | AD2 |
| Number of Residues | 9 |
| Details | binding site for Poly-Saccharide residues NAG C 502 through MAN C 506 bound to ASN C 149 |
| Chain | Residue |
| C | ASN149 |
| C | ARG192 |
| C | SER195 |
| C | ARG196 |
| C | ASN197 |
| C | SER211 |
| C | ARG213 |
| L | TYR418 |
| L | ASP500 |
| site_id | AD3 |
| Number of Residues | 3 |
| Details | binding site for Mono-Saccharide NAG D 501 bound to ASN D 80 |
| Chain | Residue |
| D | PRO78 |
| D | ASN80 |
| D | HIS119 |
| site_id | AD4 |
| Number of Residues | 9 |
| Details | binding site for Poly-Saccharide residues NAG D 502 through MAN D 506 bound to ASN D 149 |
| Chain | Residue |
| D | ASN149 |
| D | ARG192 |
| D | SER195 |
| D | ARG196 |
| D | ASN197 |
| D | SER211 |
| D | ARG213 |
| M | TYR418 |
| M | ASP500 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for Mono-Saccharide NAG E 501 bound to ASN E 80 |
| Chain | Residue |
| E | PRO78 |
| E | ASN80 |
| E | HIS119 |
| site_id | AD6 |
| Number of Residues | 10 |
| Details | binding site for Poly-Saccharide residues NAG E 502 through MAN E 506 bound to ASN E 149 |
| Chain | Residue |
| E | ARG213 |
| N | ASN417 |
| N | TYR418 |
| N | ASP500 |
| E | ASN149 |
| E | ARG192 |
| E | SER195 |
| E | ARG196 |
| E | ASN197 |
| E | SER211 |
Functional Information from PROSITE/UniProt
| site_id | PS00236 |
| Number of Residues | 15 |
| Details | NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CmMdLrrYPlDeqnC |
| Chain | Residue | Details |
| B | CYS136-CYS150 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1145 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:24909990 |
| Chain | Residue | Details |
| B | GLN1-TYR220 | |
| B | ARG269-THR279 | |
| C | GLN1-TYR220 | |
| C | ARG269-THR279 | |
| D | GLN1-TYR220 | |
| D | ARG269-THR279 | |
| E | GLN1-TYR220 | |
| E | ARG269-THR279 | |
| A | GLN1-TYR220 | |
| A | ARG269-THR279 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 430 |
| Details | TRANSMEM: Helical |
| Chain | Residue | Details |
| B | PHE221-ILE242 | |
| B | ALA246-LEU268 | |
| B | ALA280-VAL302 | |
| B | TRP426-VAL447 | |
| C | PHE221-ILE242 | |
| C | ALA246-LEU268 | |
| C | ALA280-VAL302 | |
| C | TRP426-VAL447 | |
| D | PHE221-ILE242 | |
| D | ALA246-LEU268 | |
| D | ALA280-VAL302 | |
| D | TRP426-VAL447 | |
| E | PHE221-ILE242 | |
| E | ALA246-LEU268 | |
| E | ALA280-VAL302 | |
| E | TRP426-VAL447 | |
| A | PHE221-ILE242 | |
| A | ALA246-LEU268 | |
| A | ALA280-VAL302 | |
| A | TRP426-VAL447 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:24909990 |
| Chain | Residue | Details |
| B | ASN243-ASP245 | |
| C | ASN243-ASP245 | |
| D | ASN243-ASP245 | |
| E | ASN243-ASP245 | |
| A | ASN243-ASP245 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24909990 |
| Chain | Residue | Details |
| D | PHE200 | |
| E | ASP95 | |
| E | PHE200 | |
| A | ASP95 | |
| A | PHE200 | |
| B | ASP95 | |
| B | PHE200 | |
| C | ASP95 | |
| C | PHE200 | |
| D | ASP95 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24909990, ECO:0007744|PDB:4COF |
| Chain | Residue | Details |
| B | GLU155 | |
| C | GLU155 | |
| D | GLU155 | |
| E | GLU155 | |
| A | GLU155 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 15 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990 |
| Chain | Residue | Details |
| B | ASN8 | |
| B | ASN80 | |
| B | ASN149 | |
| C | ASN8 | |
| C | ASN80 | |
| C | ASN149 | |
| D | ASN8 | |
| D | ASN80 | |
| D | ASN149 | |
| E | ASN8 | |
| E | ASN80 | |
| E | ASN149 | |
| A | ASN8 | |
| A | ASN80 | |
| A | ASN149 |
