6QFA
CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Phase Plate | VOLTA PHASE PLATE |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.49 |
Number of Particle | 315183 |
Software | RELION,EPU,Gctf,UCSF Chimera,PHENIX |
Symmetry | C5 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |